RABGAP1L (NM_014857) Human Untagged Clone

SKU SC318295

RABGAP1L (untagged)-Human RAB GTPase activating protein 1-like (RABGAP1L), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: RABGAP1L
• Synonyms: HHL; TBC1D18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318295 representing NM_014857.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGAGGTCAGAGCTTCATTACAGAAGGTTAGTGGATCATCTGATTCTGTGGCTACAATGAACAGTGAA; GAATTTGTTTTGGTTCCTCAGTATGCAGATGATAATTCTACAAAACATGAAGAAAAACCTCAACTGAAG; ATAGTTTCTAATGGTGATGAACAATTGGAAAAAGCCATGGAAGAGATTTTGAGAGATTCCGAGAAAAGG; CCAAGCAGTCTTCTTGTTGATTGTCAAAGTTCCAGTGAGATTTCAGACCATTCGTTTGGAGATATTCCA; GCCAGCCAAACAAATAAGCCATCTCTTCAGTTAATTTTGGATCCGTCTAACACAGAAATTTCTACACCC; AGACCATCTTCTCCAGGTGGACTACCTGAAGAAGATAGTGTTTTATTTAATAAACTGACCTACTTAGGA; TGTATGAAGGTTTCTTCCCCACGTAATGAAGTAGAGGCTTTACGGGCAATGGCAACCATGAAATCTTCC; AGTCAATACCCCTTTCCTGTTACCCTGTATGTACCAAATGTTCCAGAAGGTTCTGTGAGAATTATAGAC; CAATCCAGCAATGTGGAGATAGCATCTTTTCCAATCTATAAGGTGTTATTCTGTGCACGTGGACATGAC; GGAACAACAGAGAGCAATTGCTTTGCATTTACAGAGAGTTCCCATGGTTCGGAAGAATTTCAGATACAT; GTTTTCTCCTGTGAAATTAAAGAGGCAGTAAGCAGAATTTTGTACAGTTTCTGTACAGCATTCAAACGT; TCTTCCAGACAAGTGTCTGATGTTAAAGACTCAGTTATTCCTACCCCCGACAGTGATGTGTTTACCTTC; AGTGTCTCCTTGGAGGTAAAAGAAGACGATGGAAAAGGAAACTTTAGCCCTGTGCCTAAGGATAGAGAT; AAATTTTATTTCAAATTAAAGCAAGGAATAGAGAAGAAGGTTGTGATTACAGTGCAGCAACTTTCTAAC; AAAGAATTAGCTATTGAAAGATGTTTTGGAATGTTATTAAGCCCAGGTCGAAACGTGAAGAACAGTGAC; ATGCATTTACTGGATATGGAATCCATGGGAAAGAGCTATGATGGGAGAGCTTATGTCATCACTGGCATG; TGGAACCCCAATGCACCAGTATTTCTGGCACTTAACGAGGAAACCCCAAAAGATAAGCAAGTATACATG; ACTGTGGCAGTGGATATGGTAGTCACAGAGGTGGTGGAGCCTGTTCGCTTTCTCCTGGAGACAGTAGTC; CGTGTGTACCCTGCAAATGAGCGATTTTGGTATTTCAGCAGAAAGACTTTCACAGAGACTTTCTTCATG; AGATTGAAACAGTCTGAGGGAAAAGGCCATACCAATGCTGGAGATGCAATATATGAGGTGGTGAGTCTA; CAGCGAGAGTCTGACAAGGAGGAACCAGTCACTCCTACTAGTGGAGGGGGTCCAATGTCACCCCAGGAT; GATGAAGCAGAAGAGGAGAGTGATAATGAACTCTCAAGTGGAACAGGTGATGTGTCTAAGGATTGTCCT; GAGAAGATCCTGTATTCTTGGGGAGAGTTGCTAGGAAAATGGCACAGTAACCTTGGTGCACGACCGAAA; GGGCTGTCTACTCTGGTGAAGAGTGGTGTCCCTGAAGCATTGAGGGCAGAGGTATGGCAGTTATTGGCA; GGCTGCCATGACAACCAGGCAATGCTGGATAGATACCGAATTCTTATCACAAAGGACTCAGCCCAGGAG; AGTGTTATTACTCGAGATATTCATCGTACATTTCCCGCACATGATTACTTTAAAGATACTGGAGGAGAT; GGTCAAGAATCGCTCTATAAGATCTGCAAGGCCTACTCTGTGTATGATGAAGACATTGGGTACTGTCAA; GGGCAGTCTTTTCTTGCTGCTGTATTACTGCTGCATATGCCAGAGGAACAAGCATTCTGTGTTTTGGTG; AAAATCATGTACGACTATGGTTTGAGAGACCTCTACAGAAACAACTTCGAAGATCTTCATTGCAAATTC; TACCAGTTGGAGAGACTAATGCAGGAACAGCTACCGGACCTGCATAGCCATTTTTCTGATCTGAACCTG; GAAGCTCATATGTATGCATCCCAGTGGTTTCTCACTCTTTTTACTGCCAAGTTCCCACTCTGCATGGTG; TTCCACATCATTGACTTACTGCTTTGTGAGGGTTTGAACATAATCTTTCATGTAGCTTTGGCTCTCCTA; AAGACCTCAAAGGAAGACCTTCTGCAGGCTGATTTTGAAGGTGCTTTAAAGTTCTTTAGAGTTCAGCTT; CCAAAGAGATACAGGGCAGAGGAAAATGCAAGAAGACTGATGGAGCAGGCTTGCAATATTAAAGTACCA; ACCAAGAAGCTGAAGAAATATGAGAAAGAATATCAGACAATGCGAGAGAGTCAGCTGCAACAGGAAGAC; CCAATGGATAGATACAAGTTTGTATATTTGTAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_014857
• Insert Size: 2448 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014857.4
• RefSeq Size: 3022 bp
• RefSeq ORF: 2448 bp
• Locus ID: 9910
• UniProt ID: Q5R372
• Cytogenetics: 1q25.1
• MW: 92.5 kDa
• Summary: GTP-hydrolysis activating protein (GAP) for small GTPase RAB22A, converting active RAB22A-GTP to the inactive form RAB22A-GDP (PubMed:16923123). Plays a role in endocytosis and intracellular protein transport. Recruited by ANK2 to phosphatidylinositol 3-phosphate (PI3P)-positive early endosomes, where it inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) contains novel exon structure at both its 5' and 3' ends, and thus differs in both UTRs and in the 5' and 3' coding regions, compared to variant 4. The encoded isoform (A) has distinct N- and C-termini, and is significantly longer than isoform C.