Meprin beta (MEP1B) (NM_005925) Human Untagged Clone

SKU SC318181

MEP1B (untagged)-Human meprin A, beta (MEP1B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Meprin beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318181 representing NM_005925.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGATTTATGGAATCTGTCTTGGTTTCTGTTCTTGGATGCTCTTCTCGTGATTTCTGGCTTGGCAACT; CCAGAAAACTTTGATGTAGATGGCGGAATGGACCAGGACATATTTGATATCAATGAAGGTTTGGGACTG; GATCTTTTTGAGGGTGACATCAGACTTGATAGGGCACAAATTAGAAATTCCATCATTGGAGAAAAGTAT; AGATGGCCTCATACCATTCCATATGTTCTAGAAGATAGCTTGGAAATGAATGCTAAGGGAGTTATCCTC; AATGCATTTGAACGTTATCGCCTTAAAACATGTATTGACTTTAAGCCTTGGGCTGGAGAAACAAACTAT; ATATCAGTGTTCAAGGGCAGTGGCTGCTGGTCTTCAGTAGGAAATAGGCGGGTTGGGAAGCAAGAACTT; TCCATCGGGGCAAACTGTGACCGAATAGCAACAGTTCAACACGAGTTCCTCCACGCTCTGGGATTCTGG; CATGAGCAGTCGCGTTCTGACCGGGATGACTATGTCAGGATAATGTGGGACAGAATTCTGTCAGGCAGA; GAGCACAATTTTAACACCTATAGTGACGATATATCAGATTCCCTGAATGTTCCCTATGATTACACTTCA; GTAATGCACTACAGTAAAACTGCATTCCAAAATGGAACAGAGCCGACAATTGTCACAAGAATCTCAGAC; TTTGAGGATGTGATCGGCCAACGAATGGATTTCAGTGACTCTGATCTCCTAAAGTTGAATCAACTGTAT; AACTGCTCCTCTTCCTTGAGTTTTATGGACTCGTGCAGTTTTGAACTGGAAAATGTGTGTGGCATGATC; CAAAGTTCAGGAGATAATGCTGACTGGCAACGGGTTTCACAGGTTCCCAGGGGGCCAGAGAGTGATCAC; TCCAACATGGGCCAGTGCCAAGGTTCTGGTTTCTTCATGCATTTCGATAGCAGCTCTGTAAATGTGGGG; GCCACAGCAGTGCTGGAAAGTAGAACGCTGTACCCTAAAAGAGGATTTCAGTGCCTGCAATTTTACTTA; TATAACAGTGGCAGTGAAAGTGATCAACTGAACATCTATATCAGGGAGTATTCTGCAGACAATGTGGAT; GGCAATTTAACCCTTGTGGAAGAAATAAAAGAAATACCCACTGGGAGCTGGCAACTTTATCATGTAACA; TTGAAAGTGACCAAGAAGTTTAGAGTGGTGTTTGAAGGACGCAAAGGCTCTGGTGCATCACTGGGTGGT; CTGTCTATTGATGACATCAATCTTTCGGAAACACGGTGCCCTCATCATATCTGGCATATAAGGAATTTC; ACACAGTTCATTGGCAGCCCAAATGGAACTCTGTATAGCCCTCCATTTTACTCTTCTAAAGGTTATGCC; TTTCAGATTTACTTAAATCTAGCCCATGTGACTAATGCAGGGATATATTTCCACTTGATCTCTGGAGCC; AATGATGATCAATTACAGTGGCCATGTCCTTGGCAACAAGCCACAATGACACTTTTGGATCAAAATCCT; GACATTCGACAGCGTATGTCCAATCAGCGGAGTATAACTACAGACCCATTTATGACCACCGATAATGGA; AACTATTTCTGGGACAGGCCTTCTAAAGTGGGAACAGTGGCTTTGTTCTCTAATGGAACTCAGTTTAGA; AGAGGTGGGGGCTATGGAACCAGTGCCTTTATAACCCACGAAAGGCTGAAAAGCAGAGATTTTATAAAA; GGAGATGATGTTTATATCCTACTGACAGTGGAAGACATATCTCACCTCAACTCTACACAAATCCAGCTA; ACACCAGCCCCTAGTGTTCAAGACCTCTGCTCAAAAACCACCTGTAAAAATGACGGTGTCTGCACTGTT; CGAGATGGCAAAGCTGAGTGCAGGTGCCAGTCAGGGGAAGACTGGTGGTACATGGGAGAAAGGTGTGAA; AAGAGAGGCTCCACCCGAGACACCATAGTCATTGCTGTTTCATCTACTGTTGCTGTGTTTGCCTTGATG; CTGATCATCACCCTTGTCAGTGTCTATTGCACCAGGAAGAAATATCGTGAAAGGATGAGCTCAAATCGA; CCAAATTTGACTCCGCAAAATCAGCATGCTTTTTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_005925
• Insert Size: 2106 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005925.2
• RefSeq Size: 2327 bp
• RefSeq ORF: 2106 bp
• Locus ID: 4225
• UniProt ID: Q16820
• Cytogenetics: 18q12.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 79.6 kDa
• Summary: Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine. [provided by RefSeq, Oct 2011]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).