YTHDF2 (NM_016258) Human Untagged Clone

CAT#: SC317991

YTHDF2 (untagged)-Human YTH domain family, member 2 (YTHDF2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: YTHDF2
• Synonyms: CAHL; DF2; HGRG8; NY-REN-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317991 representing NM_016258.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCGGCCAGCAGCCTCTTGGAGCAGAGACCAAAAGGTCAAGGAAACAAAGTACAAAATGGATCTGTA
  CATCAAAAGGATGGATTAAACGATGATGATTTTGAACCTTACTTGAGTCCACAGGCAAGGCCCAATAAT
  GCATATACTGCCATGTCAGATTCCTACTTACCCAGTTACTACAGTCCCTCCATTGGCTTCTCCTATTCT
  TTGGGTGAAGCTGCTTGGTCTACGGGGGGTGACACAGCCATGCCCTACTTAACTTCTTATGGACAGCTG
  AGCAACGGAGAGCCCCACTTCCTACCAGATGCAATGTTTGGGCAACCAGGAGCCCTAGGTAGCACTCCA
  TTTCTTGGTCAGCATGGTTTTAATTTCTTTCCCAGTGGGATTGACTTCTCAGCATGGGGAAATAACAGT
  TCTCAGGGACAGTCTACTCAGAGCTCTGGATATAGTAGCAATTATGCTTATGCACCTAGCTCCTTAGGT
  GGAGCCATGATTGATGGACAGTCAGCTTTTGCCAATGAGACCCTCAATAAGGCTCCTGGCATGAATACT
  ATAGACCAAGGGATGGCAGCACTGAAGTTGGGTAGCACAGAAGTTGCAAGCAATGTTCCAAAAGTTGTA
  GGTTCTGCTGTTGGTAGCGGGTCCATTACTAGTAACATCGTGGCTTCCAATAGTTTGCCTCCAGCCACC
  ATTGCTCCTCCAAAACCAGCATCTTGGGCTGATATTGCTAGCAAGCCTGCAAAACAGCAACCTAAACTG
  AAGACCAAGAATGGCATTGCAGGGTCAAGTCTTCCGCCACCCCCGATAAAGCATAACATGGATATTGGA
  ACTTGGGATAACAAGGGTCCCGTTGCAAAAGCCCCCTCACAGGCTTTGGTTCAGAATATAGGTCAGCCA
  ACCCAGGGGTCTCCTCAGCCTGTAGGTCAGCAGGCTAACAATAGCCCACCAGTGGCTCAGGCATCAGTA
  GGGCAACAGACACAGCCATTGCCTCCACCTCCACCACAGCCTGCCCAGCTTTCAGTCCAGCAACAGGCA
  GCTCAGCCAACCCGCTGGGTAGCACCTCGGAACCGTGGCAGTGGGTTCGGTCATAATGGGGTGGATGGT
  AATGGAGTAGGACAGTCTCAGGCTGGTTCTGGATCTACTCCTTCAGAACCCCACCCAGTGTTGGAGAAG
  CTTCGGTCCATTAATAACTATAACCCCAAAGATTTTGACTGGAATCTGAAACATGGCCGGGTTTTCATC
  ATTAAGAGCTACTCTGAGGACGATATTCACCGTTCCATTAAGTATAATATTTGGTGCAGCACAGAGCAT
  GGTAACAAGAGACTGGATGCTGCTTATCGTTCCATGAACGGGAAAGGCCCCGTTTACTTACTTTTCAGT
  GTCAACGGCAGTGGACACTTCTGTGGCGTGGCAGAAATGAAATCTGCTGTGGACTACAACACATGTGCA
  GGTGTGTGGTCCCAGGACAAATGGAAGGGTCGTTTTGATGTCAGGTGGATTTTTGTGAAGGACGTTCCC
  AATAGCCAACTGCGACACATTCGCCTAGAGAACAACGAGAATAAACCAGTGACCAACTCTAGGGACACT
  CAGGAAGTGCCTCTGGAAAAGGCTAAGCAGGTGTTGAAAATTATAGCCAGCTACAAGCACACCACTTCC
  ATTTTTGATGACTTCTCACACTATGAGAAACGCCAAGAGGAAGAAGAAAGTGTTAAAAAGGAACGTCAA
  GGTCGTGGGAAATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_016258
• Insert Size: 1740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016258.2
• RefSeq Size: 3073 bp
• RefSeq ORF: 1740 bp
• Locus ID: 51441
• UniProt ID: Q9Y5A9
• Cytogenetics: 1p35.3
• Domains: YTH
• MW: 62.3 kDa
• Gene Summary: This gene encodes a member of the YTH (YT521-B homology) superfamily containing YTH domain. The YTH domain is typical for the eukaryotes and is particularly abundant in plants. The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a YTH domain, this protein has a proline rich region which may be involved in signal transduction. An Alu-rich domain has been identified in one of the introns of this gene, which is thought to be associated with human longevity. In addition, reciprocal translocations between this gene and the Runx1 (AML1) gene on chromosome 21 has been observed in patients with acute myeloid leukemia. This gene was initially mapped to chromosome 14, which was later turned out to be a pseudogene. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Oct 2012]
  Transcript Variant: This variant (1) encodes the longer isoform (1).