MUC20 (NM_152673) Human Untagged Clone

SKU SC317912

MUC20 (untagged)-Human mucin 20, cell surface associated (MUC20), transcript variant L

Product Data

• Type: Human Untagged Clone
• Target Symbol: MUC20
• Synonyms: MUC-20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317912 representing NM_152673.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGGCTGTCTCTGGGGTCTGGCTCTGCCCCTTTTCTTCTTCTGCTGGGAGGTTGGGGTCTCTGGGAGC; TCTGCAGGCCCCAGCACCCGCAGAGCAGACACTGCGATGACAACGGACGACACAGAAGTGCCCGCTATG; ACTCTAGCACCGGGCCACGCCGCTCTGGAAACTCAAACGCTGAGCGCTGAGACCTCTTCTAGGGCCTCA; ACCCCAGCCGGCCCCATTCCAGAAGCAGAGACCAGGGGAGCCAAGAGAATTTCCCCTGCAAGAGAGACC; AGGAGTTTCACAAAAACATCTCCCAACTTCATGGTGCTGATCGCCACCTCCGTGGAGACATCAGCCGCC; AGTGGCAGCCCCGAGGGAGCTGGAATGACCACAGTTCAGACCATCACAGGCAGTGATCCCGAGGAAGCC; ATCTTTGACACCCTTTGCACCGATGACAGCTCTGAAGAGGCAAAGACACTCACAATGGACATATTGACA; TTGGCTCACACCTCCACAGAAGCTAAGGGCCTGTCCTCAGAGAGCAGTGCCTCTTCCGACGGCCCCCAT; CCAGTCATCACCCCGTCACGGGCCTCAGAGAGCAGCGCCTCTTCCGACGGCCCCCATCCAGTCATCACC; CCGTCACGGGCCTCAGAGAGCAGCGCCTCTTCCGACGGCCCCCATCCAGTCATCACCCCGTCATGGTCC; CCGGGATCTGATGTCACTCTCCTCGCTGAAGCCCTGGTGACTGTCACAAACATCGAGGTTATTAATTGC; AGCATCACAGAAATAGAAACAACAACTTCCAGCATCCCTGGGGCCTCAGACATAGATCTCATCCCCACG; GAAGGGGTGAAGGCCTCGTCCACCTCCGATCCACCAGCTCTGCCTGACTCCACTGAAGCAAAACCACAC; ATCACTGAGGTCACAGCCTCTGCCGAGACCCTGTCCACAGCCGGCACCACAGAGTCAGCTGCACCTCAT; GCCACGGTTGGGACCCCACTCCCCACTAACAGCGCCACAGAAAGAGAAGTGACAGCACCCGGGGCCACG; ACCCTCAGTGGAGCTCTGGTCACAGTTAGCAGGAATCCCCTGGAAGAAACCTCAGCCCTCTCTGTTGAG; ACACCAAGTTACGTCAAAGTCTCAGGAGCAGCTCCGGTCTCCATAGAGGCTGGGTCAGCAGTGGGCAAA; ACAACTTCCTTTGCTGGGAGCTCTGCTTCCTCCTACAGCCCCTCGGAAGCCGCCCTCAAGAACTTCACC; CCTTCAGAGACACCGACCATGGACATCGCAACCAAGGGGCCCTTCCCCACCAGCAGGGACCCTCTTCCT; TCTGTCCCTCCGACTACAACCAACAGCAGCCGAGGGACGAACAGCACCTTAGCCAAGATCACAACCTCA; GCGAAGACCACGATGAAGCCCCCAACAGCCACGCCCACGACTGCCCGGACGAGGCCGACCACAGACGTG; AGTGCAGGTGAAAATGGAGGTTTCCTCCTCCTGCGGCTGAGTGTGGCTTCCCCGGAAGACCTCACTGAC; CCCAGAGTGGCAGAAAGGCTGATGCAGCAGCTCCACCGGGAACTCCACGCCCACGCGCCTCACTTCCAG; GTCTCCTTACTGCGTGTCAGGAGAGGCTAA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_152673
• Insert Size: 1617 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152673.3
• RefSeq Size: 2098 bp
• RefSeq ORF: 1617 bp
• Locus ID: 200958
• UniProt ID: Q8N307
• Cytogenetics: 3q29
• MW: 55.2 kDa
• Summary: This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified. [provided by RefSeq, Apr 2014]; Transcript Variant: This variant (2) lacks nine mucin tandem repeat sequences, and also has additional polymorphisms in the coding region and 3' UTR, compared to variant 1. The encoded isoform (b) is shorter than isoform a. This variant includes three mucin tandem repeats and is represented on the ALT_REF_LOCI_2 and ALT_REF_LOCI_4 alternate haplotypes of the reference genome.