ADPGK (NM_031284) Human Untagged Clone

CAT#: SC317832

ADPGK (untagged)-Human ADP-dependent glucokinase (ADPGK), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADPGK
• Synonyms: 2610017G09Rik; ADP-GK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317832 representing NM_031284.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGCTGTGGCGCGGCTCCGCGTACGCGGGCTTCCTGGCGCTGGCCGTGGGCTGCGTCTTCCTGCTG
  GAGCCAGAGCTGCCAGGCTCGGCGCTGCGCTCTCTCTGGAGCTCGCTGTGTCTGGGGCCCGCGCCTGCG
  CCCCCGGGACCCGTCTCCCCCGAGGGCCGGTTGGCGGCAGCCTGGGACGCGCTTATCGTGCGGCCAGTC
  CGGCGCTGGCGCCGCGTGGCAGTGGGAGTCAATGCATGTGTTGATGTGGTGCTCTCAGGGGTGAAGCTC
  TTGCAGGCACTTGGCCTTAGTCCTGGGAATGGGAAAGATCACAGCATTCTGCATTCAAGGAATGATCTG
  GAAGAAGCCTTCATTCACTTCATGGGGAAGGGAGCAGCTGCTGAGCGCTTCTTCAGTGATAAGGAAACT
  TTTCACGACATTGCCCAGGTTGCGTCAGAGTTCCCAGGAGCCCAGCACTATGTAGGAGGAAATGCAGCT
  TTAATTGGACAGAAATTTGCAGCCAACTCAGATTTAAAGGTTCTTCTTTGCGGTCCAGTTGGTCCAAAG
  CTACATGAGCTTCTTGATGACAATGTCTTTGTTCCACCAGAGTCATTGCAGGAAGTGGATGAGTTCCAC
  CTCATTTTAGAGTATCAAGCAGGGGAGGAGTGGGGCCAGTTAAAAGCTCCCCATGCCAACCGATTCATC
  TTCTCTCACGACCTCTCCAACGGGGCCATGAATATGCTGGAGGTGTTTGTGTCTAGCCTGGAGGAGTTT
  CAGCCAGACCTGGTGGTCCTCTCTGGATTGCACATGATGGAGGGACAAAGCAAGGAGCTCCAGAGGAAG
  AGACTCTTGGAGGTTGTAACCTCCATTTCTGACATCCCCACTGGTATTCCAGTTCACCTAGAGCTGGCC
  AGTATGACTAACAGGGAGCTCATGAGCAGCATTGTCCATCAGGTCTTTCCCGCGGTGACTTCCCTTGGG
  CTGAATGAACAGGAGCTGTTATTTCTCACCCAGTCAGCCTCTGGACCTCACTCTTCTCTCTCTTCCTGG
  AACGGTGTTCCTGATGTGGGCATGGTCAGTGACATCCTCTTCTGGATCTTGAAAGAACATGGGAGGAGT
  AAAAGCAGAGCCTCGGATCTCACCAGGATCCATTTCCACACGCTGGTCTACCACATCCTGGCAACTGTG
  GATGGACACTGGGCCAACCAGCTGGCAGCCGTGGCTGCAGGAGCTCGTGTGGCTGGGACACAGGCCTGC
  GCCACAGAAACCATAGACACCAGCCGAGTGTCTCTGAGGGCACCCCAAGAGTTCATGACTTCCCATTCG
  GAGGCAGGCTCCAGGATTGTATTAAACCCAAACAAGCCAGTAGTAGAATGGCACAGAGAGGGAATATCC
  TTCCACTTCACACCAGTATTGGTGTGTAAAGACCCCATTCGAACTGTAGGCCTTGGAGATGCCATTTCA
  GCCGAAGGACTCTTCTATTCGGAAGTACACCCTCACTATTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_031284
• Insert Size: 1491 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031284.4
• RefSeq Size: 2565 bp
• RefSeq ORF: 1491 bp
• Locus ID: 83440
• UniProt ID: Q9BRR6
• Cytogenetics: 15q24.1
• Domains: ADP_PFK_GK
• MW: 54 kDa
• Gene Summary: ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (1) encodes the full length predicted protein.