ICA1 (NM_004968) Human Untagged Clone

SKU SC317806

ICA1 (untagged)-Human islet cell autoantigen 1, 69kDa (ICA1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: ICA1
• Synonyms: ICA69; ICAp69
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317806 representing NM_004968.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGTCAGGACACAAATGCAGTTATCCCTGGGACTTACAGGATCGATATGCTCAAGATAAGTCAGTTGTA; AATAAGATGCAACAGAAATATTGGGAGACGAAGCAGGCCTTTATTAAAGCCACAGGGAAGAAGGAAGAT; GAACATGTTGTTGCCTCTGACGCGGACCTGGATGCCAAGCTAGAGCTGTTTCATTCAATTCAGAGAACC; TGTCTGGACTTATCGAAAGCAATTGTACTCTATCAAAAGAGGATATGTTTCTTGTCTCAAGAAGAAAAC; GAACTGGGAAAATTTCTTCGATCCCAAGGTTTCCAAGATAAAACCAGAGCAGGAAAGATGATGCAAGCG; ACAGGAAAGGCCCTCTGCTTTTCTTCCCAGCAAAGGTTGGCCTTACGAAATCCTTTGTGTCGATTTCAC; CAAGAAGTGGAGACTTTTCGGCATCGGGCCATCTCAGATACTTGGCTGACGGTGAACCGCATGGAACAG; TGCAGGACGGAATATAGAGGAGCACTATTATGGATGAAGGACGTGTCTCAGGAGCTTGATCCAGACCTC; TACAAGCAAATGGAGAAGTTCAGGAAGGTACAAACACAAGTGCGCCTTGCAAAAAAAAACTTTGACAAA; TTGAAGATGGATGTTTGTCAAAAAGTGGATCTTCTTGGAGCGAGCAGATGCAATCTCTTGTCTCACATG; CTAGCAACATACCAGACCACTCTGCTTCATTTTTGGGAGAAAACTTCTCACACTATGGCAGCCATCCAT; GAGAGTTTCAAAGGTTATCAACCATATGAATTTACTACTTTAAAGAGCTTACAAGACCCTATGAAAAAA; TTAGTTGAGAAAGAAGAGAAGAAGAAAATCAACCAGCAGGAAAGTACAGATGCAGCCGTGCAGGAGCCG; AGCCAATTAATTTCATTAGAGGAAGAAAACCAGCGCAAGGAATCCTCTAGTTTTAAGACTGAAGATGGA; AAAAGTATTTTATCTGCCTTAGACAAAGGCTCTACACATACTGCATGCTCAGGACCCATAGATGAACTA; TTAGACATGAAATCTGAGGAAGGTGCTTGCCTGGGACCAGTGGCAGGGACCCCGGAACCTGAAGGTGCT; GACAAAGATGACCTGCTGCTGTTGAGTGAGATCTTCAATGCTTCCTCCTTGGAAGAGGGCGAGTTCAGC; AAAGAGTGGGCCGCTGTGTTTGGAGACGGCCAAGTGAAGGAGCCAGTGCCCACTATGGCCCTGGGAGAG; CCAGACCCCAAGGCCCAGACAGGCTCAGGTTTCCTTCCTTCGCAGCTTTTAGACCAAAATATGAAAGAC; TTACAGGCCTCGCTACAAGAACCTGCTAAGGCTGCCTCAGACCTGACTGCCTGGTTCAGCCTCTTCGCT; GACCTCGACCCACTCTCAAATCCTGATGCTGTTGGGAAAACCGATAAAGAACACGAATTGCTCAATGCA; TGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_004968
• Insert Size: 1452 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004968.3
• RefSeq Size: 2458 bp
• RefSeq ORF: 1452 bp
• Locus ID: 3382
• UniProt ID: Q05084
• Cytogenetics: 7p21.3
• Domains: ICA69
• Protein Pathways: Type I diabetes mellitus
• MW: 54.6 kDa
• Summary: This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]; Transcript Variant: This variant (2) has an alternate first exon (5' UTR) compared to variant 3. Variants 1, 2, 3, 5, and 6 all encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.