TRIOBP (NM_138632) Human Untagged Clone

SKU SC317694

TRIOBP (untagged)-Human TRIO and F-actin binding protein (TRIOBP), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRIOBP
• Synonyms: DFNB28; dJ37E16.4; HRIHFB2122; TAP68; TARA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC317694 representing NM_138632.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGGCGGATGGAAGGGGCCGGGGCAGCGTCGGGGAAAGGAAGGGCCGGAGGCGCGGCGGCGGGCGGCC; GAGAGGGGCGGCGGCGGCGGCGGCGGCGGGGTTCCCGCGCCGCGGAGCCCGGCCCGAGAGCCGCGTCCA; CGTTCCTGCCTCCTGCTCCCGCCGCCCTGGGGCGCCGCCATGACGCCCGATCTGCTCAACTTCAAGAAG; GGATGGATGTCGATCTTGGACGAGCCTGGAGAGCCTCCCTCCCCCTCGCTCACCACCACCTCTACTTCG; CAGTGGAAGAAACATTGGTTTGTGCTGACAGATTCAAGTCTCAAATATTACAGAGACTCCACTGCTGAG; GAGGCAGATGAGCTGGATGGTGAGATCGACCTGCGTTCCTGCACGGATGTCACTGAGTACGCGGTGCAG; CGCAACTATGGCTTCCAGATCCACACCAAGGATGCTGTCTATACCTTGTCGGCCATGACCTCAGGCATC; CGGCGGAACTGGATCGAGGCTCTGAGAAAGACCGTACGTCCAACTTCAGCCCCAGATGTCACCAAGCTC; TCGGACTCTAACAAGGAGAACGCGCTGCACAGCTACAGCACCCAGAAGGGCCCCCTGAAGGCAGGGGAG; CAGCGGGCGGGCTCTGAGGTCATCAGCCGGGGTGGCCCTCGGAAGGCGGACGGGCAGCGTCAGGCCTTG; GACTACGTGGAGCTCTCGCCGCTGACCCAGGCTTCCCCGCAGCGGGCCCGCACCCCAGCCCGCACTCCT; GACCGCCTGGCCAAGCAGGAGGAGCTGGAGCGGGACCTGGCCCAGCGCTCCGAGGAGCGGCGCAAGTGG; TTTGAGGCCACAGACAGCAGGACCCCAGAGGTGCCTGCTGGTGAGGGGCCGCGCCGGGGCCTGGGTGCC; CCCCTGACTGAGGACCAGCAAAACCGGCTTAGTGAGGAGATCGAGAAGAAGTGGCAGGAGCTGGAGAAG; CTGCCCCTGCGGGAGAATAAGCGGGTGCCCCTCACTGCCCTGCTCAACCAAAGCCGCGGAGAGCGCCGA; GGGCCCCCAAGTGACGGCCACGAGGCACTGGAGAAGGAGGTTCAGGCTCTTCGGGCCCAGCTGGAGGCG; TGGCGTCTCCAAGGGGAGGCTCCTCAGAGTGCACTGAGATCCCAGGAGGATGGCCACATCCCCCCGGGC; TACATCTCACAGCTGGTGGGCGTGATCACTGTGCCCGTTTTACAGACAAGGCCACTGAGCTCTGAGAGG; TTATGTGACTTGCCCAAGGTCACCCCGCCTGCAGGTCTCAAAGGTGGGATTTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_138632
• Insert Size: 1296 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_138632.2
• RefSeq Size: 1743 bp
• RefSeq ORF: 1296 bp
• Locus ID: 11078
• UniProt ID: Q9H2D6
• Cytogenetics: 22q13.1
• Domains: PH
• MW: 47.6 kDa
• Summary: This gene encodes a protein with an N-terminal pleckstrin homology domain and a C-terminal coiled-coil region. The protein interacts with trio, which is involved with neural tissue development and controlling actin cytoskeleton organization, cell motility and cell growth. The protein also associates with F-actin and stabilizes F-actin structures. Mutations in this gene have been associated with a form of autosomal recessive nonsyndromic deafness. Multiple alternatively spliced transcript variants that would encode different isoforms have been found for this gene, however some transcripts may be subject to nonsense-mediated decay (NMD). [provided by RefSeq, Nov 2008]; Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR, and coding region (compared to variant 6), resulting in a protein that has shorter and distinct N- and C-termini when it is compared to isoform 6.