FMN1 (NM_001103184) Human Untagged Clone

CAT#: SC316979

FMN1 (untagged)-Human formin 1 (FMN1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FMN1
• Synonyms: FMN; LD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC316979 representing NM_001103184.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGAACGTTGATAATTCTCTGGATGGCAGTGATGTGTCAGAACCAGCAAAACCAGAAGCCGGACTT
  GAAGTGGCTCAGTCGATCCTGAGTAAGTTCTCTATGAAATCCCTATTTGGGTTTACGAGTAAATTAGAA
  TCTGTGAATCCAGAAGAGGAAGATGCTGTGCTGAAAGCATTCCATAGTTTAGACGTGAATCCCACATCT
  CAGCAGGATGATTCCAGCAATGGCTTGGATCCACAGGAAGCAGGGTCTCGGGTTTCACCTGACCTCGGA
  AATGATGAGAAAATTGCAAGCGTGGAGACAGAATCAGAGGGAAGTCAGAGAAAAGAAGCAGGGACATCT
  CTCCTTGCTCAAGAGCTGCTTCCACTGTCTACTTTGAAAGGGACAAAAGACGATGTCATTTGTGTTCGT
  GGGACCCTGGTGCACACCACCAGTGATTCTGACTCTGATGATGGTGGCCAGGAGCCAGAGGAGGGAAGC
  AGCACCAATGGCCCAAAGTCCCCTAGTGGTGTTTTATCTGAGCCATCTCAGGAGTCCAAAGAAAACCCA
  GGAGGTTTCAGGGAAAATACTGTCACTGGGGAAATGAATGGTGCAGAGCTCTGTGCAGAAGATCCTCAA
  AGGATTCCACCTGAGATGAGCAGCAAACTGGAAGCTGGCAACGGTGGTCTTCAGACAGAGCGTCGCCCC
  AGCCAGGACCAGGTGGGAGAAGAAGGGTCCCAAGACCTACCTGCAGTAACAAACCAGAATTCGAGTGTT
  GGTATCACTGAGAGTGCCTCTTCTAAAAAAGAAGTCTCTGGAGAGAAGTCATTCCAACTTCCAGCTTTT
  TTCAGTGGGCTTCGTGTGCTGAAGAAGGGGGCTACCGCTGAGGGAGGAGAGACCATCACGGAAATTAAA
  CCAAAGGATGGGGACTTAGCTTTGCTCAAATTGACGCAGCCTGTGCAGAAGTCCTTAGTGCAGGCAGGG
  CTGCAGACAGTGAAGAGTGAGAAGAAAGCAACTGATCCGAAGGCCACTCCCACTCTCCTGGAGCAGCTC
  TCTCTGCTGCTCAACATTGACATGCCCAAAACTGAACCGAAGGGAGCAGACCCTGAGTCGCCCAGGAGA
  GAAGAGATGGGCTGCAATGCTGACCAAGAGAGCCAGAGCGGCCCTGGAGTACCACAAACCCAGGGTGGT
  GAGGTCAAGCCAAAGTCGCCAGAGACAGCCCTGGAGGCTTTTAAAGCCCTATTCATCCGGCCCCCCAGA
  AAGGGGACCACAGCTGACACCTCTGAGCTGGAAGCTCTCAAGCGCAAGATGAGACATGAGAAGGAGTCG
  CTAAGAGCTGTGTTTGAACGGTCCAATTCTAAGCCAGCGGATGGCCCCTCTGATTCCAAAAGTCCTGAC
  CACAGCCTGACTGAGCAGGATGACAGGACTCCTGGCAGACTTCAAGCTGTCTGGCCACCCCCAAAGACA
  AAAGACACAGAAGAAAAAGTGGGACTGAAGTACACTGAAGCAGAATACCAAGCTGCTATTTTACACTTG
  AAGAGGGAGCACAAAGAAGAAATTGAAAACCTGCAGGCACAGTTTGAACTTCGGGCATTTCATATCCGG
  GGCGAGCATGCAATGATAACAGCGAGACTAGAAGAAACCATTGAAAATCTGAAACACGAGCTAGAACAC
  AGATGGCGAGGGGGTTGTGAAGAGAGGAAAGATGTGTGCATTTCCACCGATGATGACTGCCCTCCAAAG
  ACCTTCAGAAATGTGTGCGTCCAGACAGACAGAGAGACCTTCCTCAAGCCCTGTGAAAGTGAAAGCAAG
  ACAACCAGAAGTAATCAATTAGTACCCAAAAAGCTGAATATCTCCTCTTTAAGCCAGCTCTCACCCCCA
  AATGACCACAAAGACATCCATGCAGCACTCCAGCCAATGGAGGGCATGGCATCAAATCAGCAGAAGGCA
  TTGCCTCCGCCTCCCGCATCCATCCCTCCCCCTCCGCCCCTCCCCTCAGGACTTGGATCTTTGTCTCCC
  GCACCTCCAATGCCACCTGTGAGTGCTGGGCCACCGCTACCACCTCCGCCTCCTCCACCGCCACCTCTA
  CCTCCACCTTCAAGTGCTGGACCTCCACCACCCCCACCCCCACCCCCACTTCCTAACTCCCCTGCCCCA
  CCCAACCCTGGAGGGCCTCCTCCTGCTCCACCACCCCCAGGACTTGCACCCCCACCTCCCCCTGGACTC
  TTCTTTGGACTTGGCTCTTCTTCCAGTCAATGTCCTCGAAAACCAGCCATCGAGCCCAGTTGTCCCATG
  AAGCCTTTATATTGGACTAGGATACAAATAAGTGATAGGAGCCAAAATGCTACACCAACCTTATGGGAC
  TCCTTAGAAGAACCTGACATTCGGGACCCCAGTGAATTTGAGTATTTATTCTCCAAAGACACAACTCAA
  CAGAAGAAAAAACCTCTGTCAGAGACTTATGAGAAGAAAAACAAGGTCAAAAAGATCATCAAATTGTTG
  GATGGAAAACGATCTCAAACTGTGGGAATCTTGATATCTAGTTTACATTTAGAAATGAAGGATATCCAA
  CAGGCCATTTTCAATGTGGATGACTCCGTGGTTGATCTGGAGACCCTGGCAGCCTTATATGAAAACAGA
  GCCCAAGAGGATGAGCTGGTTAAAATAAGAAAGTATTACGAGACATCCAAAGAAGAAGAACTGAAGCTG
  CTGGATAAACCTGAGCAATTTTTACATGAGTTAGCCCAGATTCCTAATTTTGCTGAACGTGCCCAGTGC
  ATAATCTTCAGATCTGTCTTTTCTGAGGGTATCACCTCCTTGCACAGAAAGGTAGAGATCATCACGCGA
  GCTTCTAAGGACTTGCTGCACGTGAAGAGCGTGAAGGATATTTTAGCTCTCATCTTGGCTTTTGGAAAT
  TATATGAATGGAGGAAATAGGACTCGGGGACAAGCCGATGGATATAGCTTAGAAATTCTGCCCAAACTC
  AAGGATGTCAAAAGTCGGGATAATGGGATTAATCTGGTGGACTACGTTGTTAAGTATTACCTGCGTTAC
  TATGATCAGGAAGCTGGAACAGAAAAGAGTGTTTTCCCCTTGCCGGAACCACAGGATTTCTTTCTGGCC
  TCCCAAGTCAAGTTTGAAGACCTCATAAAAGATTTGAGAAAACTGAAGAGGCAACTAGAAGCAAGTGAG
  AAACAGATGGTGGTGGTGTGCAAGGAGTCCCCAAAGGAGTATCTCCAGCCTTTCAAGGACAAACTAGAG
  GAGTTCTTCCAAAAAGCCAAAAAAGAGCATAAGATGGAAGAAAGTCACTTGGAGAATGCACAGAAAAGT
  TTTGAAACAACAGTACGATATTTTGGGATGAAGCCAAAGTCTGGTGAGAAGGAGATCACACCCAGCTAC
  GTGTTTATGGTGTGGTATGAGTTCTGCAGTGACTTCAAGACAATTTGGAAACGGGAGAGTAAAAACATA
  TCTAAAGAAAGATTGAAAATGGCTCAGGAATCAGTCAGCAAGTTGACTTCAGAGAAGAAAGTGGAGACA
  AAGAAAATCAATCCCACTGCTAGCCTGAAAGAAAGACTGCGTCAGAAGGAAGCCAGTGTGACCACTAAC
  TAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001103184
• Insert Size: 3591 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001103184.3
• RefSeq Size: 12521 bp
• RefSeq ORF: 3591 bp
• Locus ID: 342184
• UniProt ID: Q68DA7
• Cytogenetics: 15q13.3
• MW: 132.2 kDa
• Gene Summary: This gene belongs to the formin homology family and encodes a protein that has a role in the formation of adherens junction and the polymerization of linear actin cables. The homologous gene in mouse is associated with limb deformity. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (2) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (b) has a distinct and shorter N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.