FMN1 (NM_001103184) Human Tagged ORF Clone

CAT#: RC216074

FMN1 (Myc-DDK-tagged)-Human formin 1 (FMN1)

Product Images

Circular map for RC216074

• 

  Western blot validation of overexpression lysate (Cat# LY420203) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216074 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FMN1
• Synonyms: FMN; LD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216074 representing NM_001103184
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACGTTGATAATTCTCTGGATGGCAGTGATGTGTCAGAACCAGCAAAACCAGAAGCCGGACTTG
  AAGTGGCTCAGTCGATCCTGAGTAAGTTCTCTATGAAATCCCTATTTGGGTTTACGAGTAAATTAGAATC
  TGTGAATCCAGAAGAGGAAGATGCTGTGCTGAAAGCATTCCATAGTTTAGACGTGAATCCCACATCTCAG
  CAGGATGATTCCAGCAATGGCTTGGATCCACAGGAAGCAGGGTCTCGGGTTTCACCTGACCTCGGAAATG
  ATGAGAAAATTGCAAGCGTGGAGACAGAATCAGAGGGAAGTCAGAGAAAAGAAGCAGGGACATCTCTCCT
  TGCTCAAGAGCTGCTTCCACTGTCTACTTTGAAAGGGACAAAAGACGATGTCATTTGTGTTCGTGGGACC
  CTGGTGCACACCACCAGTGATTCTGACTCTGATGATGGTGGCCAGGAGCCAGAGGAGGGAAGCAGCACCA
  ATGGCCCAAAGTCCCCTAGTGGTGTTTTATCTGAGCCATCTCAGGAGTCCAAAGAAAACCCAGGAGGTTT
  CAGGGAAAATACTGTCACTGGGGAAATGAATGGTGCAGAGCTCTGTGCAGAAGATCCTCAAAGGATTCCA
  CCTGAGATGAGCAGCAAACTGGAAGCTGGCAACGGTGGTCTTCAGACAGAGCGTCGCCCCAGCCAGGACC
  AGGTGGGAGAAGAAGGGTCCCAAGACCTACCTGCAGTAACAAACCAGAATTCGAGTGTTGGTATCACTGA
  GAGTGCCTCTTCTAAAAAAGAAGTCTCTGGAGAGAAGTCATTCCAACTTCCAGCTTTTTTCAGTGGGCTT
  CGTGTGCTGAAGAAGGGGGCTACCGCTGAGGGAGGAGAGACCATCACGGAAATTAAACCAAAGGATGGGG
  ACTTAGCTTTGCTCAAATTGACGCAGCCTGTGCAGAAGTCCTTAGTGCAGGCAGGGCTGCAGACAGTGAA
  GAGTGAGAAGAAAGCAACTGATCCGAAGGCCACTCCCACTCTCCTGGAGCAGCTCTCTCTGCTGCTCAAC
  ATTGACATGCCCAAAACTGAACCGAAGGGAGCAGACCCTGAGTCGCCCAGGAGAGAAGAGATGGGCTGCA
  ATGCTGACCAAGAGAGCCAGAGCGGCCCTGGAGTACCACAAACCCAGGGTGGTGAGGTCAAGCCAAAGTC
  GCCAGAGACAGCCCTGGAGGCTTTTAAAGCCCTATTCATCCGGCCCCCCAGAAAGGGGACCACAGCTGAC
  ACCTCTGAGCTGGAAGCTCTCAAGCGCAAGATGAGACATGAGAAGGAGTCGCTAAGAGCTGTGTTTGAAC
  GGTCCAATTCTAAGCCAGCGGATGGCCCCTCTGATTCCAAAAGTCCTGACCACAGCCTGACTGAGCAGGA
  TGACAGGACTCCTGGCAGACTTCAAGCTGTCTGGCCACCCCCAAAGACAAAAGACACAGAAGAAAAAGTG
  GGACTGAAGTACACTGAAGCAGAATACCAAGCTGCTATTTTACACTTGAAGAGGGAGCACAAAGAAGAAA
  TTGAAAACCTGCAGGCACAGTTTGAACTTCGGGCATTTCATATCCGGGGCGAGCATGCAATGATAACAGC
  GAGACTAGAAGAAACCATTGAAAATCTGAAACACGAGCTAGAACACAGATGGCGAGGGGGTTGTGAAGAG
  AGGAAAGATGTGTGCATTTCCACCGATGATGACTGCCCTCCAAAGACCTTCAGAAATGTGTGCGTCCAGA
  CAGACAGAGAGACCTTCCTCAAGCCCTGTGAAAGTGAAAGCAAGACAACCAGAAGTAATCAATTAGTACC
  CAAAAAGCTGAATATCTCCTCTTTAAGCCAGCTCTCACCCCCAAATGACCACAAAGACATCCATGCAGCA
  CTCCAGCCAATGGAGGGCATGGCATCAAATCAGCAGAAGGCATTGCCTCCGCCTCCCGCATCCATCCCTC
  CCCCTCCGCCCCTCCCCTCAGGACTTGGATCTTTGTCTCCCGCACCTCCAATGCCACCTGTGAGTGCTGG
  GCCACCGCTACCACCTCCGCCTCCTCCACCGCCACCTCTACCTCCACCTTCAAGTGCTGGACCTCCACCA
  CCCCCACCCCCACCCCCACTTCCTAACTCCCCTGCCCCACCCAACCCTGGAGGGCCTCCTCCTGCTCCAC
  CACCCCCAGGACTTGCACCCCCACCTCCCCCTGGACTCTTCTTTGGACTTGGCTCTTCTTCCAGTCAATG
  TCCTCGAAAACCAGCCATCGAGCCCAGTTGTCCCATGAAGCCTTTATATTGGACTAGGATACAAATAAGT
  GATAGGAGCCAAAATGCTACACCAACCTTATGGGACTCCTTAGAAGAACCTGACATTCGGGACCCCAGTG
  AATTTGAGTATTTATTCTCCAAAGACACAACTCAACAGAAGAAAAAACCTCTGTCAGAGACTTATGAGAA
  GAAAAACAAGGTCAAAAAGATCATCAAATTGTTGGATGGAAAACGATCTCAAACTGTGGGAATCTTGATA
  TCTAGTTTACATTTAGAAATGAAGGATATCCAACAGGCCATTTTCAATGTGGATGACTCCGTGGTTGATC
  TGGAGACCCTGGCAGCCTTATATGAAAACAGAGCCCAAGAGGATGAGCTGGTTAAAATAAGAAAGTATTA
  CGAGACATCCAAAGAAGAAGAACTGAAGCTGCTGGATAAACCTGAGCAATTTTTACATGAGTTAGCCCAG
  ATTCCTAATTTTGCTGAACGTGCCCAGTGCATAATCTTCAGATCTGTCTTTTCTGAGGGTATCACCTCCT
  TGCACAGAAAGGTAGAGATCATCACGCGAGCTTCTAAGGACTTGCTGCACGTGAAGAGCGTGAAGGATAT
  TTTAGCTCTCATCTTGGCTTTTGGAAATTATATGAATGGAGGAAATAGGACTCGGGGACAAGCCGATGGA
  TATAGCTTAGAAATTCTGCCCAAACTCAAGGATGTCAAAAGTCGGGATAATGGGATTAATCTGGTGGACT
  ACGTTGTTAAGTATTACCTGCGTTACTATGATCAGGAAGCTGGAACAGAAAAGAGTGTTTTCCCCTTGCC
  GGAACCACAGGATTTCTTTCTGGCCTCCCAAGTCAAGTTTGAAGACCTCATAAAAGATTTGAGAAAACTG
  AAGAGGCAACTAGAAGCAAGTGAGAAACAGATGGTGGTGGTGTGCAAGGAGTCCCCAAAGGAGTATCTCC
  AGCCTTTCAAGGACAAACTAGAGGAGTTCTTCCAAAAAGCCAAAAAAGAGCATAAGATGGAAGAAAGTCA
  CTTGGAGAATGCACAGAAAAGTTTTGAAACAACAGTACGATATTTTGGGATGAAGCCAAAGTCTGGTGAG
  AAGGAGATCACACCCAGCTACGTGTTTATGGTGTGGTATGAGTTCTGCAGTGACTTCAAGACAATTTGGA
  AACGGGAGAGTAAAAACATATCTAAAGAAAGATTGAAAATGGCTCAGGAATCAGTCAGCAAGTTGACTTC
  AGAGAAGAAAGTGGAGACAAAGAAAATCAATCCCACTGCTAGCCTGAAAGAAAGACTGCGTCAGAAGGAA
  GCCAGTGTGACCACTAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216074 representing NM_001103184
  Red=Cloning site Green=Tags(s)
  
  MENVDNSLDGSDVSEPAKPEAGLEVAQSILSKFSMKSLFGFTSKLESVNPEEEDAVLKAFHSLDVNPTSQ
  QDDSSNGLDPQEAGSRVSPDLGNDEKIASVETESEGSQRKEAGTSLLAQELLPLSTLKGTKDDVICVRGT
  LVHTTSDSDSDDGGQEPEEGSSTNGPKSPSGVLSEPSQESKENPGGFRENTVTGEMNGAELCAEDPQRIP
  PEMSSKLEAGNGGLQTERRPSQDQVGEEGSQDLPAVTNQNSSVGITESASSKKEVSGEKSFQLPAFFSGL
  RVLKKGATAEGGETITEIKPKDGDLALLKLTQPVQKSLVQAGLQTVKSEKKATDPKATPTLLEQLSLLLN
  IDMPKTEPKGADPESPRREEMGCNADQESQSGPGVPQTQGGEVKPKSPETALEAFKALFIRPPRKGTTAD
  TSELEALKRKMRHEKESLRAVFERSNSKPADGPSDSKSPDHSLTEQDDRTPGRLQAVWPPPKTKDTEEKV
  GLKYTEAEYQAAILHLKREHKEEIENLQAQFELRAFHIRGEHAMITARLEETIENLKHELEHRWRGGCEE
  RKDVCISTDDDCPPKTFRNVCVQTDRETFLKPCESESKTTRSNQLVPKKLNISSLSQLSPPNDHKDIHAA
  LQPMEGMASNQQKALPPPPASIPPPPPLPSGLGSLSPAPPMPPVSAGPPLPPPPPPPPPLPPPSSAGPPP
  PPPPPPLPNSPAPPNPGGPPPAPPPPGLAPPPPPGLFFGLGSSSSQCPRKPAIEPSCPMKPLYWTRIQIS
  DRSQNATPTLWDSLEEPDIRDPSEFEYLFSKDTTQQKKKPLSETYEKKNKVKKIIKLLDGKRSQTVGILI
  SSLHLEMKDIQQAIFNVDDSVVDLETLAALYENRAQEDELVKIRKYYETSKEEELKLLDKPEQFLHELAQ
  IPNFAERAQCIIFRSVFSEGITSLHRKVEIITRASKDLLHVKSVKDILALILAFGNYMNGGNRTRGQADG
  YSLEILPKLKDVKSRDNGINLVDYVVKYYLRYYDQEAGTEKSVFPLPEPQDFFLASQVKFEDLIKDLRKL
  KRQLEASEKQMVVVCKESPKEYLQPFKDKLEEFFQKAKKEHKMEESHLENAQKSFETTVRYFGMKPKSGE
  KEITPSYVFMVWYEFCSDFKTIWKRESKNISKERLKMAQESVSKLTSEKKVETKKINPTASLKERLRQKE
  ASVTTN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001103184
• ORF Size: 3588 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001103184.4
• RefSeq Size: 3757 bp
• RefSeq ORF: 3591 bp
• Locus ID: 342184
• UniProt ID: Q68DA7
• Cytogenetics: 15q13.3
• MW: 132 kDa
• Gene Summary: This gene belongs to the formin homology family and encodes a protein that has a role in the formation of adherens junction and the polymerization of linear actin cables. The homologous gene in mouse is associated with limb deformity. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]