C5ORF33 (NADK2) (NM_001085411) Human Untagged Clone

CAT#: SC316220

NADKD1 (untagged)-Human chromosome 5 open reading frame 33 (C5orf33), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NADK2
• Synonyms: C5orf33; DECRD; MNADK; NADKD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC316220 representing NM_001085411.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACTTGCTACCGAGGCTTCTTGCTGGGCAGCTGTTGTCGCGTGGCGGGCGGCCGGGCGGCGGCGCTG
  CGGGGACCGGGTGCGGGAGGCCCCGCCGCGCGGCCCCGGCTGGGCGGTGACGGTGGCGGCCGGCGGCAC
  CTGGGGCAGGGGCAGCCGCGCGAGCTGGCGGGCTGTGGCAGCCGCGCGGACGGCGGCTTCCGCCCCTCC
  CGGGTGGTGGTGGTGGCCAAAACCACCCGGTACGAGTTCGAGCAGCAGCGGTACCGTTACGCGGAGCTC
  TCGGAGGAGGACCTGAAGCAGCTGCTTGCATTGAAAGGCTCTAGTTACAGTGGACTTCTTGAACGACAT
  CATATTCACACCAAAAATGTAGAACATATTATAGATAGTTTACGGAATGAGGGAATTGAGGTTCGTCTA
  GTAAAGAGGAGAGAATATGATGAAGAGACTGTTCGATGGGCAGATGCTGTCATAGCTGCAGGAGGTGAT
  GGCACAATGCTGCTGGCAGCGAGTAAAGTCTTGGACAGACTTAAACCAGTTATAGGGGTAAACACTGAT
  CCAGAACGGTCTGAGGGTCATTTATGCCTGCCCGTTCGATATACACATTCCTTTCCAGAAGCCTTACAG
  AAGTTCTATCGTGGTGAGTTCAGGTGGTTGTGGAGGCAGAGAATCAGGTTATACCTTGAAGGGACTGGC
  ATAAACCCTGTACCTGTGGACCTTCACGAGCAGCAGCTAAGCTTGAATCAGCACAATAGAGCCCTTAAC
  ATTGAAAGAGCTCATGATGAAAGGTCTGAGGCTTCAGGACCCCAACTTCTGCCAGTGAGAGCACTAAAT
  GAAGTCTTCATTGGGGAGAGTCTGTCATCCAGGGCTTCCTACTATGAGATTTCAGTTGATGATGGTCCA
  TGGGAAAAACAGAAGAGTTCAGGGCTCAATTTGTGTACTGGAACAGGATCAAAGGCCTGGTCATTCAAT
  ATTAACAGGGTTGCAACTCAGGCTGTAGAAGATGTTTTAAATATTGCAAAACGACAAGGAAATTTGAGT
  CTTCCATTGAACAGAGAATTGGTAGAGAAAGTAACAAATGAATATAATGAATCACTGCTCTACAGTCCG
  GAAGAACCAAAAATACTTTTCAGTATTCGAGAACCAATAGCAAATAGAGTTTTCTCAAGCAGTCGTCAG
  CGTTGTTTCTCCTCAAAGGTTTGTGTTCGTTCTCGTTGTTGGGATGCCTGTATGGTTGTGGATGGAGGA
  ACTTCTTTTGAGTTTAATGATGGTGCAATTGCTTCGATGATGATCAATAAAGAAGATGAGCTTCGAACT
  GTGCTTCTTGAACAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001085411
• Insert Size: 1329 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001085411.2
• RefSeq Size: 3897 bp
• RefSeq ORF: 1329 bp
• Locus ID: 133686
• UniProt ID: Q4G0N4
• Cytogenetics: 5p13.2
• MW: 49.4 kDa
• Gene Summary: This gene encodes a mitochondrial kinase that catalyzes the phosphorylation of NAD to yield NADP. Mutations in this gene result in 2,4-dienoyl-CoA reductase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).