C5ORF33 (NADK2) (NM_001085411) Human Tagged ORF Clone

CAT#: RC214147

NADKD1 (Myc-DDK-tagged)-Human chromosome 5 open reading frame 33 (C5orf33), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY421290) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214147 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NADK2 protein (Cat# TP314147). The protein was produced from HEK293T cells transfected with NADK2 cDNA clone (Cat# RC214147) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NADK2
• Synonyms: C5orf33; DECRD; MNADK; NADKD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214147 representing NM_001085411
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTGCTACCGAGGCTTCTTGCTGGGCAGCTGTTGTCGCGTGGCGGGCGGCCGGGCGGCGGCGCTGC
  GGGGACCGGGTGCGGGAGGCCCCGCCGCGCGGCCCCGGCTGGGCGGTGACGGTGGCGGCCGGCGGCACCT
  GGGGCAGGGGCAGCCGCGCGAGCTGGCGGGCTGTGGCAGCCGCGCGGACGGCGGCTTCCGCCCCTCCCGG
  GTGGTGGTGGTGGCCAAAACCACCCGGTACGAGTTCGAGCAGCAGCGGTACCGTTACGCGGAGCTCTCGG
  AGGAGGACCTGAAGCAGCTGCTTGCATTGAAAGGCTCTAGTTACAGTGGACTTCTTGAACGACATCATAT
  TCACACCAAAAATGTAGAACATATTATAGATAGTTTACGGAATGAGGGAATTGAGGTTCGTCTAGTAAAG
  AGGAGAGAATATGATGAAGAGACTGTTCGATGGGCAGATGCTGTCATAGCTGCAGGAGGTGATGGCACAA
  TGCTGCTGGCAGCGAGTAAAGTCTTGGACAGACTTAAACCAGTTATAGGGGTAAACACTGATCCAGAACG
  GTCTGAGGGTCATTTATGCCTGCCCGTTCGATATACACATTCCTTTCCAGAAGCCTTACAGAAGTTCTAT
  CGTGGTGAGTTCAGGTGGTTGTGGAGGCAGAGAATCAGGTTATACCTTGAAGGGACTGGCATAAACCCTG
  TACCTGTGGACCTTCACGAGCAGCAGCTAAGCTTGAATCAGCACAATAGAGCCCTTAACATTGAAAGAGC
  TCATGATGAAAGGTCTGAGGCTTCAGGACCCCAACTTCTGCCAGTGAGAGCACTAAATGAAGTCTTCATT
  GGGGAGAGTCTGTCATCCAGGGCTTCCTACTATGAGATTTCAGTTGATGATGGTCCATGGGAAAAACAGA
  AGAGTTCAGGGCTCAATTTGTGTACTGGAACAGGATCAAAGGCCTGGTCATTCAATATTAACAGGGTTGC
  AACTCAGGCTGTAGAAGATGTTTTAAATATTGCAAAACGACAAGGAAATTTGAGTCTTCCATTGAACAGA
  GAATTGGTAGAGAAAGTAACAAATGAATATAATGAATCACTGCTCTACAGTCCGGAAGAACCAAAAATAC
  TTTTCAGTATTCGAGAACCAATAGCAAATAGAGTTTTCTCAAGCAGTCGTCAGCGTTGTTTCTCCTCAAA
  GGTTTGTGTTCGTTCTCGTTGTTGGGATGCCTGTATGGTTGTGGATGGAGGAACTTCTTTTGAGTTTAAT
  GATGGTGCAATTGCTTCGATGATGATCAATAAAGAAGATGAGCTTCGAACTGTGCTTCTTGAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214147 representing NM_001085411
  Red=Cloning site Green=Tags(s)
  
  MTCYRGFLLGSCCRVAGGRAAALRGPGAGGPAARPRLGGDGGGRRHLGQGQPRELAGCGSRADGGFRPSR
  VVVVAKTTRYEFEQQRYRYAELSEEDLKQLLALKGSSYSGLLERHHIHTKNVEHIIDSLRNEGIEVRLVK
  RREYDEETVRWADAVIAAGGDGTMLLAASKVLDRLKPVIGVNTDPERSEGHLCLPVRYTHSFPEALQKFY
  RGEFRWLWRQRIRLYLEGTGINPVPVDLHEQQLSLNQHNRALNIERAHDERSEASGPQLLPVRALNEVFI
  GESLSSRASYYEISVDDGPWEKQKSSGLNLCTGTGSKAWSFNINRVATQAVEDVLNIAKRQGNLSLPLNR
  ELVEKVTNEYNESLLYSPEEPKILFSIREPIANRVFSSSRQRCFSSKVCVRSRCWDACMVVDGGTSFEFN
  DGAIASMMINKEDELRTVLLEQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001085411
• ORF Size: 1326 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001085411.3
• RefSeq Size: 3898 bp
• RefSeq ORF: 1329 bp
• Locus ID: 133686
• UniProt ID: Q4G0N4
• Cytogenetics: 5p13.2
• MW: 49.3 kDa
• Gene Summary: This gene encodes a mitochondrial kinase that catalyzes the phosphorylation of NAD to yield NADP. Mutations in this gene result in 2,4-dienoyl-CoA reductase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]