ZFYVE19 (NM_001077268) Human Untagged Clone

SKU SC315569

ZFYVE19 (untagged)-Human zinc finger, FYVE domain containing 19 (ZFYVE19)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ZFYVE19
• Synonyms: ANCHR; MPFYVE
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001077268 edited; TCTAGGAGACAGGGGCCACGGGGAGAGCACAGCCACTCGGCGCGAAGAGCCCTCTGTACC; CCGCTTCCTCTTGAGGCCCTCGGACCGTCCAGGAAATGTCTGGGAGCCCGCCTGCGGAGG; GCATAGCGCCGACCCTCGCTCCCCGCCCAGGACCCGAATGAACCTGGAGAGCGGATGCCA; GCAGTGGCCGAGGCGCTAGGACAGGAAGGACCGCCAGACCTCTCAAGATCAGCCTTCCTC; GCCACCGTTCTCACCTCTTTGTCCGCTGCCTTCTCCTCAATGCCTAGCAGCGCGTACAGG; TCCATCTGTAAGAGCTCCTTGGTCACTGCCATGGTTCCGGCCTGACGGATTCGTACTACA; ACTCCCAAGAGTCTAAGCGCGCGTGAGGACTGCAGGCTCCGAGCGGCGCCTAGCCCTCTG; GGAATTGTGTTCTGGGTCAGGCTTGACTGACTCTGAGGGAGGCCGGCAGTCGTGAATGAA; CTACGACTCCCAGCAGCCCCCGTTGCCGCCGCTGCCGTACGCTGGCTGCAGGAGAGCGTC; CGGATTCCCTGCTCTAGGTCGCGGCGGGACAGTGCCAGTGGGCGTGTGGGGCGGGGCAGG; GCAGGGAAGGGAAGGGCGGAGCTGGGGTGAGGGTCCAAGGGGCCCAGGACTTGGCCGGCG; TGATCTCAGCTCTGCAGACCCTGCGGTGCTGGGAGCCACCATGGAGAGTAGGTGCTACGG; CTGCGCTGTCAAGTTCACCCTCTTCAAGAAGGAGTACGGCTGTAAGAATTGTGGCAGGGC; CTTCTGTTCAGGCTGCCTAAGCTTCAGTGCAGCAGTGCCTCGGACTGGGAACACCCAACA; GAAAGTCTGCAAGCAATGCCATGAGGTCCTGACCAGAGGGTCTTCTGCCAATGCCTCCAA; GTGGTCACCACCTCAGAACTATAAGAAGCGTGTGGCAGCCTTGGAAGCCAAGCAAAAGCC; CAGCACTTCCCAGAGCCAGGGACTGACACGACAAGACCAGATGATTGCTGAGCGCCTAGC; ACGACTCCGCCAGGAGAACAAGCCCAAGTTAGTCCCCTCACAGGCAGAGATAGAGGCACG; GCTGGCTGCCCTAAAGGATGAACGTCAGGGTTCCATCCCTTCCACCCAGGAAATGGAGGC; ACGACTTGCAGCGTTGCAGGGCAGAGTTCTACCTTCTCAAACCCCCCAGCCGGCACATCA; CACACCGGACACCAGGACCCAAGCCCAGCAGACACAGGATCTGCTAACGCAGCTGGCAGC; TGAGGTGGCTATCGATGAAAGCTGGAAAGGAGGAGGCCCAGCTGCCTCTCTCCAGAATGA; TCTCAACCAGGGTGGCCCAGGGAGCACTAATTCCAAGAGGCAGGCCAACTGGTCCTTGGA; GGAGGAGAAGAGCAGACTGCTGGCTGAGGCAGCACTTGAGTTGCGGGAGGAGAACACGAG; GCAGGAACGGATTCTGGCCCTGGCCAAGCGACTAGCCATGCTGCGGGGACAGGACCCCGA; GAGAGTGACCCTCCAGGACTATCGCCTCCCAGACAGTGATGACGACGAGGATGAGGAGAC; AGCCATCCAAAGAGTCCTGCAGCAGCTCACTGAAGAAGCTGCCCTGGATGAGGCAAGTGG; CTTTAACATCCCTGCAGAGCAGGCTTCTCGACCCTGGACGCAACCCCGCGGGGCAGAGCC; TGAGGCCCAGGATGTGGACCCCAGGCCTGAGGCTGAGGAAGAGGAGCTCCCCTGGTGCTG; CATCTGCAATGAGGATGCCACCCTACGCTGCGCTGGCTGCGATGGGGACCTCTTCTGTGC; CCGCTGCTTCCGAGAGGGCCATGATGCCTTTGAGCTTAAAGAGCACCAGACATCTGCCTA; CTCTCCTCCACGTGCAGGCCAAGAGCACTGAAGACACCCTGGTCCTCCCGGAAGGGCAGT; CCCACAGGCAGCGGCACCCATTTCTGGGCCCCGCCACAGGACGTCCGATGGGAGAGCTTG; TCTGGCTCTACTGATGATGGATAGGCCCCTTCCTGAGCCTTGGTGTCCCTGGAATGAGGA; AAGATTCTCCATTCGAGAGAATGACTGGGAGGGAAGAAGTCGGGGCCCTCCTATTAGAAG; CCCAGACTGGAAGTGAGAGGCATGATGGGGAGAGACCAGACTGAATCTACGGGTGAGCCC; TGTAACCTGGCTCTAGGGCACAGGCCCCTCCCCTGGCACTTAGTGGGTCTAATAAAGTAT; GTTGATTCATTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAA;
• Restriction Sites: Please inquire
• ACCN: NM_001077268
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_001077268.1.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001077268.1, NP_001070736.1
• RefSeq Size: 2293 bp
• RefSeq ORF: 1416 bp
• Locus ID: 84936
• UniProt ID: Q96K21
• Cytogenetics: 15q15.1
• Summary: Key regulator of abscission step in cytokinesis: part of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Together with CHMP4C, required to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis. Deactivation of AURKB results in dephosphorylation of CHMP4C followed by its dissociation from ZFYVE19/ANCHR and VPS4 and subsequent abscission.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).