TrkA (NTRK1) (NM_002529) Human Untagged Clone

SKU SC315454

NTRK1 (untagged)-Human neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: TrkA
• Synonyms: MTC; p140-TrkA; TRK; Trk-A; TRK1; TRKA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002529 edited; ATGCTGCGAGGCGGACGGCGCGGGCAGCTTGGCTGGCACAGCTGGGCTGCGGGGCCGGGC; AGCCTGCTGGCTTGGCTGATACTGGCATCTGCGGGCGCCGCACCCTGCCCCGATGCCTGC; TGCCCCCACGGCTCCTCGGGACTGCGATGCACCCGGGATGGGGCCCTGGATAGCCTCCAC; CACCTGCCCGGCGCAGAGAACCTGACTGAGCTCTACATCGAGAACCAGCAGCATCTGCAG; CATCTGGAGCTCCGTGATCTGAGGGGCCTGGGGGAGCTGAGAAACCTCACCATCGTGAAG; AGTGGTCTCCGTTTCGTGGCGCCAGATGCCTTCCATTTCACTCCTCGGCTCAGTCGCCTG; AATCTCTCCTTCAACGCTCTGGAGTCTCTCTCCTGGAAAACTGTGCAGGGCCTCTCCTTA; CAGGAACTGGTCCTGTCGGGGAACCCTCTGCACTGTTCTTGTGCCCTGCGCTGGCTACAG; CGCTGGGAGGAGGAGGGACTGGGCGGAGTGCCTGAACAGAAGCTGCAGTGTCATGGGCAA; GGGCCCCTGGCCCACATGCCCAATGCCAGCTGTGGTGTGCCCACGCTGAAGGTCCAGGTG; CCCAATGCCTCGGTGGATGTGGGGGACGACGTGCTGCTGCGGTGCCAGGTGGAGGGGCGG; GGCCTGGAGCAGGCCGGCTGGATCCTCACAGAGCTGGAGCAGTCAGCCACGGTGATGAAA; TCTGGGGGTCTGCCATCCCTGGGGCTGACCCTGGCCAATGTCACCAGTGACCTCAACAGG; AAGAACGTGACGTGCTGGGCAGAGAACGATGTGGGCCGGGCAGAGGTCTCTGTTCAGGTC; AACGTCTCCTTCCCGGCCAGTGTGCAGCTGCACACGGCGGTGGAGATGCACCACTGGTGC; ATCCCCTTCTCTGTGGATGGGCAGCCGGCACCGTCTCTGCGCTGGCTCTTCAATGGCTCC; GTGCTCAATGAGACCAGCTTCATCTTCACTGAGTTCCTGGAGCCCGCAGCCAATGAGACC; GTGCGGCACGGGTGTCTGCGCCTCAACCAGCCCACCCACGTCAACAACGGCAACTACACG; CTGCTGGCTGCCAACCCCTTCGGCCAGGCCTCCGCCTCCATCATGGCTGCCTTCATGGAC; AACCCTTTCGAGTTCAACCCCGAGGACCCCATCCCTGTCTCCTTCTCGCCGGTGGACACT; AACAGCACATCTGGAGACCCGGTGGAGAAGAAGGACGAAACACCTTTTGGGGTCTCGGTG; GCTGTGGGCCTGGCCGTCTTTGCCTGCCTCTTCCTTTCTACGCTGCTCCTTGTGCTCAAC; AAATGTGGACGGAGAAACAAGTTTGGGATCAACCGCCCGGCTGTGCTGGCTCCAGAGGAT; GGGCTGGCCATGTCCCTGCATTTCATGACATTGGGTGGCAGCTCCCTGTCCCCCACCGAG; GGCAAAGGCTCTGGGCTCCAAGGCCACATCATCGAGAACCCACAATACTTCAGTGATGCC; TGTGTTCACCACATCAAGCGCCGGGACATCGTGCTCAAGTGGGAGCTGGGGGAGGGCGCC; TTTGGGAAGGTCTTCCTTGCTGAGTGCCACAACCTCCTGCCTGAGCAGGACAAGATGCTG; GTGGCTGTCAAGGCACTGAAGGAGGCGTCCGAGAGTGCTCGGCAGGACTTCCAGCGTGAG; GCTGAGCTGCTCACCATGCTGCAGCACCAGCACATCGTGCGCTTCTTCGGCGTCTGCACC; GAGGGCCGCCCCCTGCTCATGGTCTTTGAGTATATGCGGCACGGGGACCTCAACCGCTTC; CTCCGATCCCATGGACCTGATGCCAAGCTGCTGGCTGGTGGGGAGGATGTGGCTCCAGGC; CCCCTGGGTCTGGGGCAGCTGCTGGCCGTGGCTAGCCAGGTCGCTGCGGGGATGGTGTAC; CTGGCGGGTCTGCATTTTGTGCACCGGGACCTGGCCACACGCAACTGTCTAGTGGGCCAG; GGACTGGTGGTCAAGATTGGTGATTTTGGCATGAGCAGGGATATCTACAGCACCGACTAT; TACCGTGTGGGAGGCCGCACCATGCTGCCCATTCGCTGGATGCCGCCCGAGAGCATCCTG; TACCGTAAGTTCACCACCGAGAGCGACGTGTGGAGCTTCGGCGTGGTGCTCTGGGAGATC; TTCACCTACGGCAAGCAGCCCTGGTACCAGCTCTCCAACACGGAGGCAATCGACTGCATC; ACGCAGGGACGTGAGTTGGAGCGGCCACGTGCCTGCCCACCAGAGGTCTACGCCATCATG; CGGGGCTGCTGGCAGCGGGAGCCCCAGCAACGCCACAGCATCAAGGATGTGCACGCCCGG; CTGCAAGCCCTGGCCCAGGCACCTCCTGTCTACCTGGATGTCCTGGGCTAG;
  5' Read Nucleotide Sequence

  >Reverse primer walk for NM_002529 unedited; GGGGNCCCACTACGTTCGTGCCCCCACTNCCACCGNAGNNCATTGGGCACCTGGNACCTT; CAGCGTGGGCACACCACAGCTGGCATTGGGCATGTGGGCCAGGGGCCCTTGCCCATGACA; CTGCAGCTTCTGTTCAGGCACTCCGCCCAGTCCCTCCTCCTCCCAGCGCTGTAGCCAGCG; CAGGGCACAAGAACAGTGCAGAGGGTTCCCCGACAGGACCAGTTCCTGTAAGGAGAGGCC; CTGCACAGTTTTCCAGGAGAGAGACTCCAGAGCGTTGAAGGAGAGATTCAGGCGACTGAG; CCGAGGAGTGAAATGGAAGGCATCTGGCGCCACGAAACGGAGACCACTCTTCACGATGGT; GAGGTTTCTCAGCTCCCCCAGGCCCCTCAGATCACGGAGCTCCAGATGCTGCAGATGCTG; CTGGTTCTCGATGTAGAGCTCAGTCAGGTTCTCTGCGCCGGGCAGGTGGTGGAGGCTATC; CAGGGCCCCATCCCGGGTGCATCGCAGTCCCGAGGAGCCGTGGGGGCAGCAGGCATCGGG; GCAGGGTGCGGCGCCCGCAGATGCCAGTATCAGCCAAGCCAGCAGGCTGCCCGGCCCCGC; AGCCCAGCTGTGCCAGCCAAGCTGCCCGCGCCGTCCGCCTCGCAGCATGCGGCCGCCCTA; TAGTGAGTCGTATTACANAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCT; CCCACCGTACACGCCCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTT; GGGAAAGTCCCCGTGATTTTTGGTGCCAAAACAAACTNCCATTGGACGTCATGGGTTGGA; GACTTGGAAATCCCCGTGAGTCAAACCGCTATCCAGCCCCATTGGTGTACTGCCCAAACC; GATCAACCTGGTAATAGCGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002529 unedited; GGCAATGGGGAGGCAACTTCCAGGCCAGNATAGCACTGGGGAGGGTACAGGGATGCCCCC; GGGACGTTCAGAAACAGCTATGACCGCGGCCGCAATCTAGAGCGTCACGAGCTAGCCCAG; GACATCCAGGTAGACAGGAGGTGCCTGGGCCAGGGCTTGCAGCCGGGCGTGCACATCCTT; GATGCTGTGGCGTTGCTGGGGCTCCCGCTGCCAGCAGCCCCGCATGATGGCGTAGACCTC; TGGTGGGCAGGCACGTGGCCGCTCCAACTCACGTCCCTGCGTGATGCAGTCGATTGCCTC; CGTGTTGGAGAGCTGGTACCAGGGCTGCTTGCCGTAGGTGAAGATCTCCCAGAGCACCAC; GCCGAAGCTCCACACGTCGCTCTCGGTGGTGAACTTACGGTACAGGATGCTCTCGGGCGG; CATCCAGCGAATGGGCAGCATGGTGCGGCCTCCCACACGGTAATAGTCGGTGCTGTAGAT; ATCCCTGCTCATGCCAAAATCACCAATCTTGACCCACCAGTCCCTGNCCCACTAGACAGT; TTGCGTGTGGCCAGGTCCCGGTGCACANAATGCAGACCCGCCAGGTACACCATCCCCGCA; GCGACCTGGCTAGCCANCGGGGGGCNAGCACCTGCCCAAACCCAGGGGGCCTGGACCCCC; ATTCTCCCCACAACCAAAACTTGGAATTAGTCCATGGAATCNNNNNAAGAAAAAAAAACC; CCTTTGTAGTCCCCTCTGGCCGCCATTTCTCTAAAAACTATAGCCGGGGGGGGCCCCTCT; TGGGCCAACCCCCCAAAAACCCCCAAGTGTGTTGGTTTTCCCCCGTGGGAAAAACCCCCA; CCCCCCCCCCGGAAAATCCTGGGAAAAACTTTTGGGACCCTCTTTTTTTTGG;
• Restriction Sites: Please inquire
• ACCN: NM_002529
• Insert Size: 2400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002529.3, NP_002520.2
• RefSeq Size: 2663 bp
• RefSeq ORF: 2391 bp
• Locus ID: 4914
• UniProt ID: P04629
• Cytogenetics: 1q23.1
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Apoptosis, Endocytosis, MAPK signaling pathway, Neurotrophin signaling pathway, Pathways in cancer, Thyroid cancer
• Summary: This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, cognitive disability and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2).