Semaphorin 3D (SEMA3D) (NM_152754) Human Untagged Clone

SKU SC314672

SEMA3D (untagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3D (SEMA3D)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Semaphorin 3D
• Synonyms: coll-2; Sema-Z2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_152754 edited; ATGAATGCTAATAAAGATGAAAGACTTAAAGCCAGAAGCCAAGATTTTCACCTTTTTCCT; GCTTTGATGATGCTAAGCATGACCATGTTGTTTCTTCCAGTCACTGGCACTTTGAAGCAA; AATATTCCAAGACTCAAGCTAACCTACAAAGACTTGCTGCTTTCAAATAGCTGTATTCCC; TTTTTGGGTTCATCAGAAGGACTGGATTTTCAAACTCTTCTCTTAGATGAGGAAAGAGGC; AGGCTGCTCTTGGGAGCCAAAGACCACATCTTTCTACTCAGTCTGGTTGACTTAAACAAA; AATTTTAAGAAGATTTATTGGCCTGCTGCAAAGGAACGGGTGGAATTATGTAAATTAGCT; GGGAAAGATGCCAATACAGAATGTGCAAATTTCATCAGAGTACTTCAGCCCTATAACAAA; ACTCACATATATGTGTGTGGAACTGGAGCATTTCATCCAATATGTGGGTATATTGATCTT; GGAGTCTACAAGGAGGATATTATATTCAAACTAGACACACATAATTTGGAGTCTGGCAGA; CTGAAATGTCCTTTCGATCCTCAGCAGCCTTTTGCTTCAGTAATGACAGATGAGTACCTC; TACTCTGGAACAGCTTCTGATTTCCTTGGCAAAGATACTGCATTCACTCGATCCCTTGGG; CCTACTCATGACCACCACTACATCAGAACTGACATTTCAGAGCACTACTGGCTCAATGGA; GCAAAATTTATTGGAACTTTCTTCATACCAGACACCTACAATCCAGATGATGATAAAATA; TATTTCTTCTTTCGTGAATCATCTCAAGAAGGCAGTACCTCCGATAAAACCATCCTTTCT; CGAGTTGGAAGAGTTTGTAAGAATGATGTAGGAGGACAACGCAGCCTGATAAACAAGTGG; ACGACTTTTCTTAAGGCCAGACTGATTTGCTCAATTCCTGGAAGTGATGGGGCAGATACT; TACTTTGATGAGCTTCAAGATATTTATTTACTCCCCACAAGAGATGAAAGAAATCCTGTA; GTATATGGAGTCTTTACTACAACCAGCTCCATCTTCAAAGGCTCTGCTGTTTGTGTGTAT; AGCATGGCTGACATCAGAGCAGTTTTTAATGGTCCATATGCTCATAAGGAAAGTGCAGAC; CATCGTTGGGTGCAGTATGATGGGAGAATTCCTTATCCACGGCCTGGTACATGTCCAAGC; AAAACCTATGACCCACTGATTAAGTCCACCCGAGATTTTCCAGATGATGTCATCAGTTTC; ATAAAGCGGCACTCTGTGATGTATAAGTCCGTATACCCAGTTGCAGGAGGACCAACGTTC; AAGAGAATCAATGTGGATTACAGACTGACACAGATAGTGGTGGATCATGTCATTGCAGAA; GATGGCCAGTACGATGTAATGTTTCTTGGAACAGACATTGGAACTGTCCTCAAAGTTGTC; AGCATTTCAAAGGAAAAGTGGAATATGGAAGAGGTAGTGCTGGAGGAGTTGCAGATATTC; AAGCACTCATCAATCATCTTGAACATGG:AATTGTCTCTGAAGCAGCAACAATTGTACAT; TGGTTCCCGAGATGGATTGGTTCAGCTCTCCTTGCACAGATGCGACACTTATGGGAAAGC; TTGCGCAGACTGTTGTCTTGCCAGAGACCCCTACTGTGCCTGGGATGGAAATGCATGCTC; TCGATATGCTCCTACTTCTAAAAGGAGAGCTAGACGCCAAGATGTAAAATATGGCGACCC; AATCACCCAGTGCTGGGACATCGAAGACAGCATTAGTCATGAAACTGCTGATGAAAAGGT; GATTTTTGGCATTGAATTTAACTCAACCTTTCTGGAATGTATACCTAAATCCCAACAAGC; AACTATTAAATGGTATATCCAGAGGTCAGGGGATGAGCATCGAGAGGAGTTGAAGCCCGA; TGAAAGAATCATCAAAACGGAATATGGGCTACTGATTCGAAGTTTGCAGAAGAAGGATTC; TGGGATGTATTACTGCAAAGCCCAGGAGCACACTTTCATCCACACCATAGTGAAGCTGAC; TTTGAATGTCATTGAGAATGAACAGATGGAAAATACCCAGAGGGCAGAGCATGAGGAGGG; GAAGGTCAAGGATCTATTGGCTGAGTCACGGTTGAGATACAAAGACTACATCCAAATCCT; TAGCAGCCCAAACTTCAGCCTCGACCAGTACTGCGAACAGATGTGGCACAGGGAGAAGCG; GAGACAGAGAAACAAGGGGGGCCCAAAGTGGAAGCACATGCAGGAAATGAAGAAGAAACG; AAATCGAAGACATCACAGAGACCTGGATGAGCTCCCTAGAGCTGTAGCCACGTAG;
• Restriction Sites: Please inquire
• ACCN: NM_152754
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_152754.1.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152754.2, NP_689967.2
• RefSeq Size: 6276 bp
• RefSeq ORF: 2334 bp
• Locus ID: 223117
• UniProt ID: O95025
• Cytogenetics: 7q21.11
• Domains: IG, PSI
• Protein Families: Transmembrane
• Protein Pathways: Axon guidance
• Summary: This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin like domain and a C-terminal basic domain. The protein encoded by this gene binds neuropilin and plays an important role in cardiovascular development. [provided by RefSeq, Aug 2016]