DNA polymerase eta (POLH) (NM_006502) Human Untagged Clone

SKU SC314503

POLH (untagged)-Human polymerase (DNA directed), eta (POLH)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DNA polymerase eta
• Synonyms: RAD30; RAD30A; XP-V; XPV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC314503 representing NM_006502.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCTACTGGACAGGATCGAGTGGTTGCTCTCGTGGACATGGACTGTTTTTTTGTTCAAGTGGAGCAG; CGGCAAAATCCTCATTTGAGGAATAAACCTTGTGCAGTTGTACAGTACAAATCATGGAAGGGTGGTGGA; ATAATTGCAGTGAGTTATGAAGCTCGTGCATTTGGAGTCACTAGAAGTATGTGGGCAGATGATGCTAAG; AAGTTATGTCCAGATCTTCTACTGGCACAAGTTCGTGAGTCCCGTGGGAAAGCTAACCTCACCAAGTAC; CGGGAAGCCAGTGTTGAAGTGATGGAGATAATGTCTCGTTTTGCTGTGATTGAACGTGCCAGCATTGAT; GAGGCTTACGTAGATCTGACCAGTGCTGTACAAGAGAGACTACAAAAGCTACAAGGTCAGCCTATCTCG; GCAGACTTGTTGCCAAGCACTTACATTGAAGGGTTGCCCCAAGGCCCTACAACGGCAGAAGAGACTGTT; CAGAAAGAGGGGATGCGAAAACAAGGCTTATTTCAATGGCTCGATTCTCTTCAGATTGATAACCTCACC; TCTCCAGACCTGCAGCTCACCGTGGGAGCAGTGATTGTGGAGGAAATGAGAGCAGCCATAGAGAGGGAG; ACTGGTTTTCAGTGTTCAGCTGGAATTTCACACAATAAGGTCCTGGCAAAACTGGCCTGTGGACTAAAC; AAGCCCAACCGCCAAACCCTGGTTTCACATGGGTCAGTCCCACAGCTCTTCAGCCAAATGCCCATTCGC; AAAATCCGTAGTCTTGGAGGAAAGCTAGGGGCCTCTGTCATTGAGATCCTAGGGATAGAATACATGGGT; GAACTGACCCAGTTCACTGAATCCCAGCTCCAGAGTCATTTTGGGGAGAAGAATGGGTCTTGGCTATAT; GCCATGTGCCGAGGGATTGAACATGATCCAGTTAAACCCAGGCAACTACCCAAAACCATTGGCTGTAGT; AAGAACTTCCCAGGAAAAACAGCTCTTGCTACTCGGGAACAGGTACAATGGTGGCTGTTGCAATTAGCC; CAGGAACTAGAGGAGAGACTGACTAAAGACCGAAATGATAATGACAGGGTAGCCACCCAGCTGGTTGTG; AGCATTCGTGTACAAGGAGACAAACGCCTCAGCAGCCTGCGCCGCTGCTGTGCCCTTACCCGCTATGAT; GCTCACAAGATGAGCCATGATGCATTTACTGTCATCAAGAACTGTAATACTTCTGGAATCCAGACAGAA; TGGTCTCCTCCTCTCACAATGCTTTTCCTCTGTGCTACAAAATTTTCTGCCTCTGCCCCTTCATCTTCT; ACAGACATCACCAGCTTCTTGAGCAGTGACCCAAGTTCTCTGCCAAAGGTGCCAGTTACCAGCTCAGAA; GCTAAGACCCAGGGAAGTGGCCCAGCGGTGACAGCCACTAAGAAAGCAACCACGTCTCTGGAATCATTC; TTCCAAAAAGCTGCAGAAAGGCAGAAAGTTAAAGAAGCTTCGCTTTCATCTCTTACTGCTCCCACTCAG; GCTCCCATGAGCAATTCACCATCCAAGCCCTCATTACCTTTTCAAACCAGTCAAAGTACAGGAACTGAG; CCCTTCTTTAAGCAGAAAAGTCTGCTTCTAAAGCAGAAACAGCTTAATAATTCTTCAGTTTCTTCCCCC; CAACAAAACCCATGGTCCAACTGTAAAGCATTACCAAACTCTTTACCAACAGAGTATCCAGGGTGTGTC; CCTGTTTGTGAAGGGGTGTCGAAGCTAGAAGAATCCTCTAAAGCAACTCCTGCAGAGATGGATTTGGCC; CACAACAGCCAAAGCATGCACGCCTCTTCAGCTTCCAAATCTGTGCTGGAGGTGACTCAGAAAGCAACC; CCAAATCCAAGTCTTCTAGCTGCTGAGGACCAAGTGCCCTGTGAGAAGTGTGGCTCCCTGGTACCGGTA; TGGGATATGCCAGAACACATGGACTATCATTTTGCATTGGAGTTGCAGAAATCCTTTTTGCAGCCCCAC; TCTTCAAACCCCCAGGTTGTTTCTGCCGTATCTCATCAAGGCAAAAGAAATCCCAAGAGCCCTTTGGCC; TGCACTAATAAACGCCCCAGGCCTGAGGGCATGCAAACATTGGAATCATTTTTTAAGCCATTAACACAT; TAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_006502
• Insert Size: 2142 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006502.2
• RefSeq Size: 8412 bp
• RefSeq ORF: 2142 bp
• Locus ID: 5429
• UniProt ID: Q9Y253
• Cytogenetics: 6p21.1
• Domains: IMS
• Protein Families: Druggable Genome
• MW: 78.4 kDa
• Summary: This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).