NSMase2 (SMPD3) (NM_018667) Human Untagged Clone

CAT#: SC314339

SMPD3 (untagged)-Human sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II) (SMPD3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NSMase2
• Synonyms: NSMASE2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC314339 representing NM_018667.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTTTTGTACACGACCCCCTTTCCTAACAGCTGTCTGTCCGCCCTGCACTGTGTGTCCTGGGCCCTT
  ATCTTTCCATGCTACTGGCTGGTGGACCGGCTCGCTGCCTCCTTCATACCCACCACCTACGAGAAGCGC
  CAGCGGGCAGACGACCCGTGCTGCCTGCAGCTGCTCTGCACTGCCCTCTTCACGCCCATCTACCTGGCC
  CTCCTGGTGGCCTCGCTGCCCTTTGCGTTTCTCGGCTTTCTCTTCTGGTCCCCACTGCAGTCGGCCCGC
  CGGCCCTACATCTATTCACGGCTGGAAGACAAGGGCCTGGCCGGTGGGGCAGCCCTGCTCAGTGAATGG
  AAGGGCACGGGGCCTGGCAAAAGCTTCTGCTTTGCCACTGCCAACGTCTGCCTCCTGCCCGACTCACTC
  GCCAGGGTCAACAACCTTTTTAACACCCAAGCGCGGGCCAAGGAGATCGGGCAGAGAATCCGCAATGGG
  GCCGCCCGGCCCCAGATCAAAATTTACATCGACTCCCCCACCAATACCTCCATCAGCGCCGCTAGCTTC
  AGCAGCCTGGTGTCACCACAGGGCGGCGATGGGGTGGCCCGGGCCGTCCCCGGGAGCATTAAGAGGACA
  GCCTCTGTGGAGTACAAGGGTGACGGTGGGCGGCACCCCGGTGACGAGGCTGCCAACGGCCCAGCCTCT
  GGGGACCCTGTCGACAGCAGCAGCCCGGAGGATGCCTGCATCGTGCGCATCGGTGGCGAGGAGGGCGGC
  CGGCCACCTGAAGCTGACGACCCTGTGCCTGGGGGCCAGGCCAGGAACGGAGCTGGCGGGGGCCCAAGG
  GGCCAGACGCCCAACCATAATCAGCAGGACGGGGATTCAGGGAGCCTGGGCAGCCCCTCGGCCTCCCGG
  GAGTCCCTGGTGAAGGGGCGAGCTGGGCCAGACACCAGTGCCAGCGGGGAGCCAGGTGCCAACAGCAAG
  CTCCTGTACAAGGCCTCGGTGGTGAAGAAGGCGGCTGCACGCAGGAGGCGGCACCCCGACGAGGCCTTC
  GACCATGAGGTCTCCGCCTTCTTCCCCGCCAACCTGGACTTCCTGTGCCTGCAGGAGGTGTTTGACAAG
  CGAGCAGCCACCAAATTGAAAGAGCAGCTGCACGGCTACTTCGAGTACATCCTGTACGACGTCGGGGTC
  TACGGCTGCCAGGGCTGCTGCAGCTTCAAGTGTCTCAACAGCGGCCTCCTCTTTGCCAGCCGCTACCCC
  ATCATGGACGTGGCCTATCACTGTTACCCCAACAAGTGTAACGACGATGCCCTGGCCTCTAAGGGAGCT
  CTGTTTCTCAAGGTGCAGGTGGGAAGCACACCTCAGGACCAAAGAATCGTCGGGTACATCGCCTGCACA
  CACCTGCATGCCCCGCAAGAGGACAGCGCCATCCGGTGTGGGCAGCTGGACCTGCTTCAGGACTGGCTG
  GCTGATTTCCGAAAATCTACCTCCTCGTCCAGCGCAGCCAACCCCGAGGAGCTGGTGGCATTTGACGTC
  GTCTGTGGAGATTTCAACTTTGATAACTGCTCCTCTGACGACAAGCTGGAGCAGCAACACTCCCTGTTC
  ACCCACTACAGGGACCCCTGCCGCCTGGGGCCTGGTGAGGAGAAGCCGTGGGCCATCGGTACTCTGCTG
  GACACGAACGGCCTGTACGATGAGGATGTGTGCACCCCCGACAACCTGCAGAAGGTCCTGGAGAGTGAG
  GAGGGCCGCAGGGAGTACCTGGCGTTTCCCACCAGCAAGAGCTCGGGCCAGAAGGGGCGGAAGGAGCTG
  CTGAAGGGCAACGGCCGGCGCATCGACTACATGCTGCATGCAGAGGAGGGGCTGTGCCCAGACTGGAAG
  GCCGAGGTGGAAGAATTCAGTTTTATCACCCAGCTGTCCGGCCTGACGGACCACCTGCCAGTAGCCATG
  CGACTGATGGTGTCTTCGGGGGAGGAGGAGGCATAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_018667
• Insert Size: 1968 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018667.3
• RefSeq Size: 5269 bp
• RefSeq ORF: 1968 bp
• Locus ID: 55512
• UniProt ID: Q9NY59
• Cytogenetics: 16q22.1
• Domains: Exo_endo_phos
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• MW: 71.1 kDa
• Gene Summary: Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.[UniProtKB/Swiss-Prot Function]