ARHGAP8 (NM_181335) Human Untagged Clone

SKU SC313443

ARHGAP8 (untagged)-Human Rho GTPase activating protein 8 (ARHGAP8), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: ARHGAP8
• Synonyms: BPGAP1; PP610
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC313443 representing NM_181335.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCTGGCCAGGATCCTGCGCTGAGCACGAGTCACCCGTTCTACGACGTGGCCAGACATGGCATTCTG; CAGGTGGCAGGGGATGACCGCTTTGGAAGACGTGTTGTCACGTTCAGCTGCTGCCGGATGCCACCCTCC; CACGAGCTGGACCACCAGCGGCTGCTGGAGTATTTGAAGTACACACTGGACCAATACGTTGAGAACGAT; TATACCATCGTCTATTTCCACTACGGGCTGAACAGCCGGAACAAGCCTTCCCTGGGCTGGCTCCAGAGC; GCATACAAGGAGTTCGATAGGAAGTACAAGAAGAACTTGAAGGCCCTCTACGTGGTGCACCCCACCAGC; TTCATCAAGGTCCTGTGGAACATCTTGAAGCCCCTCATCAGTCACAAGTTTGGGAAGAAAGTCATCTAT; TTCAACTACCTGAGTGAGCTCCACGAACACCTTAAATACGACCAGCTGGTCATCCCTCCCGAAGTTTTG; CGGTACGATGAGAAGCTCCAGAGCCTGCACGAGGGCCGGACGCCGCCTCCCACCAAGACACCACCGCCG; CGGCCCCCGCTGCCCACACAGCAGTTTGGCGTCAGTCTGCAATACCTCAAAGACAAAAATCAAGGCGAA; CTCATCCCCCCTGTGCTGAGGTTCACAGTGACGTACCTGAGAGAGAAAGGCCTGCGCACCGAGGGCCTG; TTCCGGAGATCCGCCAGCGTGCAGACCGTCCGCGAGATCCAGAGGCTCTACAACCAAGGGAAGCCCGTG; AACTTTGACGACTACGGGGACATTCACATCCCTGCCGTGATCCTGAAGACCTTCCTGCGAGAGCTGCCC; CAGCCGCTTCTGACCTTCCAGGCCTACGAGCAGATTCTCGGGATCACCTGTGTGGAGAGCAGCCTGCGT; GTCACTGGCTGCCGCCAGATCTTACGGAGCCTCCCAGAGCACAACTACGTCGTCCTCCGCTACCTCATG; GGCTTCCTGCATGCGGTGTCCCGGGAGAGCATCTTCAACAAAATGAACAGCTCTAACCTGGCCTGTGTC; TTCGGGCTGAATTTGATCTGGCCATCCCAGGGGGTCTCCTCCCTGAGTGCCCTTGTGCCCCTGAACATG; TTCACTGAACTGCTGATCGAGTACTATGAAAAGATCTTCAGCACCCCGGAGGCACCTGGGGAGCACGGC; CTGGCACCATGGGAACAGGGGAGCAGGGCAGCCCCTTTGCAGGAGGCTGTGCCACGGACACAAGCCACG; GGCCTCACCAAGCCTACCCTACCTCCGAGTCCCCTGATGGCAGCCAGAAGACGTCTCTAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_181335
• Insert Size: 1302 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_181335.2
• RefSeq Size: 1632 bp
• RefSeq ORF: 1302 bp
• Locus ID: 23779
• UniProt ID: P85298
• Cytogenetics: 22q13.31
• MW: 49.8 kDa
• Summary: This gene encodes a member of the RHOGAP family. GAP (GTPase-activating) family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. GAP proteins alternate between an active (GTP-bound) and inactive (GDP-bound) state based on the GTP:GDP ratio in the cell. This family member is a multidomain protein that functions to promote Erk activation and cell motility. Alternative splicing results in multiple transcript variants. Read-through transcripts from the upstream proline rich 5, renal (PRR5) gene into this gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene. [provided by RefSeq, Nov 2010]; Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding reigon, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.