SLC12A6 (NM_001042495) Human Untagged Clone

SKU SC311419

SLC12A6 (untagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: SLC12A6
• Synonyms: ACCPN; KCC3; KCC3A; KCC3B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC311419 representing NM_001042495.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGAGTGAGATGTCTGGGGCCACCACTTCGCTGGCAACTGTTGCACTGGATCCACCCAGTGACCGGACT; TCTCACCCCCAGGATGTCATCGAGGACCTGAGTCAGAACTCCATCACAGGGGAACACAGCCAACTGTTA; GACGACGGACATAAGAAAGCTCGAAATGCTTATCTCAATAATTCCAATTATGAAGAAGGAGATGAATAT; TTTGATAAAAATTTGGCACTCTTTGAGGAAGAAATGGACACCAGACCGAAGGTGTCTTCCCTCCTCAAC; CGCATGGCCAATTACACTAATCTGACTCAAGGAGCAAAGGAACATGAAGAGGCAGAAAACATCACTGAA; GGGAAAAAGAAGCCCACCAAGACCCCCCAAATGGGTACCTTCATGGGTGTCTACCCCCCATGTCTACAA; AATATTTTTGGAGTGATCCTTTTTTTACGCCTTACATGGGTGGTGGGCACAGCTGGAGTTCTTCAGGCT; TTTGCAATTGTCCTTATCTGCTGCTGCTGTACAATGTTGACTGCTATCTCCATGAGTGCCATTGCCACT; AATGGAGTGGTGCCAGCTGGGGGCTCATACTTTATGATTTCCCGGGCACTGGGCCCAGAGTTTGGTGGG; GCTGTTGGCCTCTGCTTTTATCTTGGTACCACATTTGCAGCAGCCATGTACATCCTTGGTGCCATTGAA; ATCTTTCTGGTCTATATCGTCCCCCGAGCTGCCATCTTTCACAGTGATGACGCACTCAAGGAATCAGCA; GCCATGCTAAATAACATGCGTGTCTACGGCACAGCTTTCTTGGTCCTTATGGTATTAGTGGTATTTATC; GGCGTACGCTATGTGAACAAGTTTGCCTCACTTTTCCTGGCCTGTGTCATTGTGTCCATCTTGGCCATC; TATGCTGGAGCCATCAAGTCTTCTTTTGCTCCTCCACACTTCCCGGTCTGCATGCTGGGTAACCGCACC; CTTTCATCAAGACACATTGACGTTTGCTCTAAGACCAAGGAAATTAACAACATGACAGTCCCATCAAAG; TTATGGGGATTCTTCTGTAACTCGAGTCAATTTTTCAATGCCACCTGTGATGAATACTTTGTTCACAAT; AACGTCACTTCAATCCAGGGCATTCCTGGATTGGCTAGTGGTATAATTACAGAGAATCTTTGGAGTAAT; TACCTACCCAAGGGAGAGATCATCGAAAAGCCTTCAGCCAAATCTTCTGATGTCTTAGGCAGCTTAAAC; CATGAATATGTTCTTGTTGACATCACCACCTCCTTCACGCTTCTGGTGGGAATCTTCTTTCCCTCTGTT; ACAGGTATCATGGCTGGATCAAACAGATCTGGAGATCTGAAAGATGCTCAGAAGTCTATTCCGATTGGT; ACTATCCTTGCCATCCTGACCACCTCCTTTGTTTATTTAAGCAATGTTGTCCTTTTTGGTGCATGTATT; GAAGGGGTTGTTCTCAGAGACAAGTTCGGTGATGCTGTGAAAGGTAATTTGGTGGTAGGCACCTTATCT; TGGCCATCCCCATGGGTGATTGTTATTGGCTCCTTCTTTTCAACATGTGGGGCTGGACTTCAGAGCCTC; ACAGGTGCACCGAGGCTGCTACAAGCTATTGCCAAGGATAACATCATACCGTTTCTGAGGGTTTTTGGC; CACAGCAAAGCCAATGGGGAACCTACCTGGGCTTTACTTCTAACTGCTGCCATTGCAGAGCTTGGAATA; CTCATTGCCTCCCTGGATCTTGTGGCCCCAATTCTTTCCATGTTTTTTCTCATGTGTTACCTCTTTGTA; AACTTGGCATGTGCCTTGCAAACATTACTTCGAACACCCAACTGGAGACCCCGATTCCGCTACTACCAT; TGGGCCCTTTCTTTCATGGGAATGAGTATCTGTCTGGCTCTGATGTTCATTTCTTCCTGGTATTATGCC; ATTGTAGCCATGGTAATAGCTGGTATGATCTACAAGTACATTGAATACCAAGGAGCTGAGAAAGAATGG; GGTGATGGTATCCGTGGGCTGTCCCTCAGTGCAGCCCGGTTTGCTTTGCTTCGATTGGAGGAAGGACCT; CCACACACTAAAAACTGGAGGCCTCAGTTGCTTGTATTACTGAAACTAGATGAAGACTTACATGTCAAG; CATCCTCGCCTCCTCACCTTTGCCTCACAGCTCAAAGCAGGAAAAGGTCTCACTATTGTGGGCTCTGTC; ATCGTGGGGAACTTCCTAGAGAACTACGGTGAAGCTTTAGCTGCTGAGCAGACCATAAAGCACCTAATG; GAGGCAGAGAAGGTAAAAGGATTCTGCCAGCTGGTGGTGGCCGCCAAGCTGAGAGAGGGCATTTCCCAC; CTCATCCAGTCATGTGGCCTTGGGGGCATGAAGCACAACACGGTGGTGATGGGCTGGCCTAATGGCTGG; CGTCAAAGCGAAGATGCCCGCGCTTGGAAGACTTTTATTGGCACAGTTCGAGTGACAACTGCTGCCCAT; CTTGCACTGCTGGTGGCTAAAAACATCTCCTTCTTTCCCAGCAATGTGGAGCAATTTTCTGAGGGCAAC; ATTGATGTGTGGTGGATTGTGCATGATGGGGGGATGCTTATGCTACTACCATTCCTACTGAAACAGCAC; AAGGTGTGGCGAAAGTGCAGCATACGGATCTTCACAGTAGCCCAATTAGAAGACAACAGTATCCAAATG; AAGAAGGACCTAGCCACCTTCCTATATCACTTACGCATTGAGGCGGAGGTAGAAGTGGTGGAGATGCAT; GACAGTGATATATCAGCATATACTTACGAGCGCACTTTGATGATGGAACAAAGGTCCCAGATGCTTCGG; CACATGCGGCTATCCAAAACAGAGCGAGACAGAGAGGCACAATTGGTGAAAGACCGAAACTCAATGCTA; CGATTGACCAGCATTGGCTCTGATGAGGACGAAGAGACAGAAACCTATCAGGAGAAGGTGCACATGACT; TGGACAAAAGACAAGTACATGGCATCCCGGGGACAAAAAGCGAAGTCAATGGAAGGATTCCAGGACCTG; CTTAACATGCGTCCGGACCAGTCCAATGTGAGGCGGATGCATACAGCAGTGAAACTCAACGAGGTTATA; GTTAACAAGTCCCATGAAGCAAAGCTGGTTTTATTGAATATGCCAGGGCCACCCCGAAACCCTGAGGGT; GATGAAAACTACATGGAGTTCCTAGAGGTGCTTACCGAGGGACTAGAGCGAGTCCTACTTGTCCGGGGT; GGTGGCAGTGAAGTGATCACCATTTATTCATAA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042495
• Insert Size: 3276 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001042495.1
• RefSeq Size: 7363 bp
• RefSeq ORF: 3276 bp
• Locus ID: 9990
• UniProt ID: Q9UHW9
• Cytogenetics: 15q14
• Protein Families: Transmembrane
• MW: 121.1 kDa
• Summary: This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (4) has an additional exon in its 5' UTR and uses an alternate in-frame splice site in the first protein coding exon, compared to variant 1. The encoded protein (isoform c) uses a downstream start codon, compared to isoform a.