HEC1 (NDC80) (NM_006101) Human Untagged Clone

SKU SC310202

NDC80 (untagged)-Human NDC80 homolog, kinetochore complex component (S. cerevisiae) (NDC80)

Product Data

• Type: Human Untagged Clone
• Target Symbol: HEC1
• Synonyms: HEC; HEC1; HsHec1; hsNDC80; KNTC2; TID3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310202 representing NM_006101.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGAAGCGCAGTTCAGTTTCCAGCGGTGGTGCTGGCCGCCTCTCCATGCAGGAGTTAAGATCCCAGGAT; GTAAATAAACAAGGCCTCTATACCCCTCAAACCAAAGAGAAACCAACCTTTGGAAAGTTGAGTATAAAC; AAACCGACATCTGAAAGAAAAGTCTCGCTATTTGGCAAAAGAACTAGTGGACATGGATCCCGGAATAGT; CAACTTGGTATATTTTCCAGTTCTGAGAAAATCAAGGACCCGAGACCACTTAATGACAAAGCATTCATT; CAGCAGTGTATTCGACAACTCTGTGAGTTTCTTACAGAAAATGGTTATGCACATAATGTGTCCATGAAA; TCTCTACAAGCTCCCTCTGTTAAAGACTTCCTGAAGATCTTCACATTTCTTTATGGCTTCCTGTGCCCC; TCATACGAACTTCCTGACACAAAGTTTGAAGAAGAGGTTCCAAGAATCTTTAAAGACCTTGGGTATCCT; TTTGCACTATCCAAAAGCTCCATGTACACAGTGGGGGCTCCTCATACATGGCCTCACATTGTGGCAGCC; TTAGTTTGGCTAATAGACTGCATCAAGATACATACTGCCATGAAAGAAAGCTCACCTTTATTTGATGAT; GGGCAGCCTTGGGGAGAAGAAACTGAAGATGGAATTATGCATAATAAGTTGTTTTTGGACTACACCATA; AAATGCTATGAGAGTTTTATGAGTGGTGCCGACAGCTTTGATGAGATGAATGCAGAGCTGCAGTCAAAA; CTGAAGGATTTATTTAATGTGGATGCTTTTAAGCTGGAATCATTAGAAGCAAAAAACAGAGCATTGAAT; GAACAGATTGCAAGATTGGAACAAGAAAGAGAAAAAGAACCGAATCGTCTAGAGTCGTTGAGAAAACTG; AAGGCTTCCTTACAAGGAGATGTTCAAAAGTATCAGGCATACATGAGCAATTTGGAGTCTCATTCAGCC; ATTCTTGACCAGAAATTAAATGGTCTCAATGAGGAAATTGCTAGAGTAGAACTAGAATGTGAAACAATA; AAACAGGAGAACACTCGACTACAGAATATCATTGACAACCAGAAGTACTCAGTTGCAGACATTGAGCGA; ATAAATCATGAAAGAAATGAATTGCAGCAGACTATTAATAAATTAACCAAGGACCTGGAAGCTGAACAA; CAGAAGTTGTGGAATGAGGAGTTAAAATATGCCAGAGGCAAAGAAGCGATTGAAACACAATTAGCAGAG; TATCACAAATTGGCTAGAAAATTAAAACTTATTCCTAAAGGTGCTGAGAATTCCAAAGGTTATGACTTT; GAAATTAAGTTTAATCCCGAGGCTGGTGCCAACTGCCTTGTCAAATACAGGGCTCAAGTTTATGTACCT; CTTAAGGAACTCCTGAATGAAACTGAAGAAGAAATTAATAAAGCCCTAAATAAAAAAATGGGTTTGGAG; GATACTTTAGAACAATTGAATGCAATGATAACAGAAAGCAAGAGAAGTGTGAGAACTCTGAAAGAAGAA; GTTCAAAAGCTGGATGATCTTTACCAACAAAAAATTAAGGAAGCAGAGGAAGAGGATGAAAAATGTGCC; AGTGAGCTTGAGTCCTTGGAGAAACACAAGCACCTGCTAGAAAGTACTGTTAACCAGGGGCTCAGTGAA; GCTATGAATGAATTAGATGCTGTTCAGCGGGAATACCAACTAGTTGTGCAAACCACGACTGAAGAAAGA; CGAAAAGTGGGAAATAACTTGCAACGTCTGTTAGAGATGGTTGCTACACATGTTGGGTCTGTAGAGAAA; CATCTTGAGGAGCAGATTGCTAAAGTTGATAGAGAATATGAAGAATGCATGTCAGAAGATCTCTCGGAA; AATATTAAAGAGATTAGAGATAAGTATGAGAAGAAAGCTACTCTAATTAAGTCTTCTGAAGAATGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_006101
• Insert Size: 1929 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006101.2
• RefSeq Size: 2209 bp
• RefSeq ORF: 1929 bp
• Locus ID: 10403
• UniProt ID: O14777
• Cytogenetics: 18p11.32
• Domains: Ndc80_HEC
• Protein Families: Druggable Genome
• MW: 73.9 kDa
• Summary: This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]