MKLN1 (NM_013255) Human Untagged Clone

SKU SC310142

MKLN1 (untagged)-Human muskelin 1, intracellular mediator containing kelch motifs (MKLN1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: MKLN1
• Synonyms: TWA2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310142 representing NM_013255.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCGGCTGGCGGAGCTGTCGCTGCGGCGCCCGAGTGCCGGCTTCTCCCCTACGCGCTACACAAGTGG; AGCTCCTTTTCCTCCACCTACCTTCCCGAGAACATTTTAGTGGACAAACCAAATGACCAATCTTCAAGA; TGGTCTTCAGAGAGCAACTATCCTCCCCAGTACTTGATTCTAAAGCTCGAAAGGCCTGCTATAGTTCAG; AATATCACATTTGGAAAATATGAGAAAACTCATGTTTGCAATTTGAAGAAATTTAAAGTCTTTGGTGGA; ATGAATGAAGAAAATATGACAGAGCTGTTGTCCAGTGGCTTAAAGAATGATTATAACAAAGAAACATTC; ACCTTGAAGCATAAAATTGATGAACAGATGTTCCCTTGTCGATTCATTAAAATAGTTCCACTCTTGTCC; TGGGGACCCAGCTTTAACTTTAGCATCTGGTATGTTGAACTTAGTGGCATTGATGATCCTGATATAGTA; CAACCTTGTCTCAACTGGTATAGCAAGTACCGTGAACAGGAAGCTATTCGCCTTTGCCTAAAACACTTC; AGACAACACAACTATACAGAAGCTTTTGAGTCACTGCAAAAGAAAACCAAGATTGCACTGGAACATCCC; ATGTTAACAGATATTCATGACAAGCTGGTGTTGAAGGGTGATTTTGATGCTTGCGAAGAGTTGATTGAA; AAGGCTGTAAATGATGGCTTGTTCAATCAGTATATCAGTCAACAGGAATATAAGCCACGATGGAGTCAA; ATCATTCCCAAAAGTACCAAAGGTGATGGGGAAGATAACCGTCCAGGAATGAGAGGAGGCCATCAGATG; GTTATTGATGTTCAAACAGAGACTGTTTATTTGTTTGGTGGCTGGGATGGAACACAAGATCTTGCTGAC; TTCTGGGCGTACAGTGTGAAGGAGAACCAGTGGACATGTATCTCTAGAGACACTGAAAAAGAGAATGGT; CCTAGTGCCAGATCGTGTCATAAAATGTGCATTGATATTCAACGGAGGCAAATCTACACATTGGGGCGT; TACTTGGATTCCTCTGTGAGGAACAGCAAATCTCTGAAAAGTGACTTCTATCGTTATGACATTGATACA; AACACATGGATGTTACTAAGTGAGGATACTGCTGCTGATGGAGGGCCGAAATTGGTGTTTGATCATCAG; ATGTGTATGGACTCAGAAAAACATATGATCTACACTTTTGGTGGTAGAATTTTGACTTGTAATGGCAGC; GTAGATGACAGCAGAGCCAGTGAACCACAATTCAGTGGCTTGTTTGCTTTCAACTGTCAATGTCAAACC; TGGAAACTTCTTCGAGAGGACTCCTGTAATGCTGGGCCTGAGGACATCCAGTCTCGAATAGGACACTGC; ATGTTATTCCACTCAAAAAATCGTTGCTTATATGTATTTGGTGGCCAGCGATCAAAGACCTATTTGAAT; GATTTCTTTAGTTATGATGTGGACTCTGATCATGTAGACATAATATCAGATGGCACCAAGAAAGACTCT; GGGATGGTTCCAATGACAGGATTTACACAGAGAGCAACTATTGATCCAGAACTGAATGAAATACACGTC; TTATCTGGACTCAGCAAAGATAAGGAAAAGAGGGAAGAAAATGTTAGAAATTCATTCTGGATTTATGAC; ATTGTGAGGAATAGTTGGTCTTGTGTCTATAAGAATGATCAAGCTGCAAAGGATAATCCAACTAAAAGT; CTTCAGGAAGAAGAACCATGTCCAAGGTTTGCCCATCAGCTTGTATACGATGAGCTACACAAGGTTCAT; TACTTATTTGGTGGGAATCCAGGAAAATCTTGCTCTCCAAAGATGAGATTAGATGACTTCTGGTCACTG; AAGTTGTGTAGACCTTCAAAAGATTATTTACTGAGGCATTGCAAGTACCTCATAAGAAAACACAGGTTT; GAAGAAAAGGCCCAAGTGGATCCCCTTAGTGCTCTGAAATATTTACAAAATGATCTTTATATAACTGTG; GATCATTCAGACCCAGAAGAGACAAAAGAGTTTCAGCTCCTGGCATCAGCTCTATTCAAATCTGGTTCA; GATTTTACAGCTCTGGGCTTTTCTGATGTGGATCACACCTATGCTCAAAGAACTCAGCTCTTTGACACC; TTAGTAAATTTCTTTCCTGACAGCATGACTCCTCCTAAAGGCAACCTGGTAGACCTCATCACACTGTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_013255
• Insert Size: 2208 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013255.4
• RefSeq Size: 11194 bp
• RefSeq ORF: 2208 bp
• Locus ID: 4289
• UniProt ID: Q9UL63
• Cytogenetics: 7q32.3
• Domains: Kelch, LisH
• MW: 84.8 kDa
• Summary: Muskelin is an intracellular protein that acts as a mediator of cell spreading and cytoskeletal responses to the extracellular matrix component thrombospondin I (MIM 188060) (Adams et al., 1998 [PubMed 9724633]).[supplied by OMIM, Mar 2008]; Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.