TBC1D4 (NM_014832) Human Untagged Clone

CAT#: SC309926

TBC1D4 (untagged)-Human TBC1 domain family, member 4 (TBC1D4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBC1D4
• Synonyms: AS160; NIDDM5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC309926 representing NM_014832.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGCCGCCCAGCTGCATTCAGGATGAGCCGTTCCCGCACCCCCTGGAGCCCGAGCCGGGCGTCTCA
  GCTCAGCCCGGCCCCGGGAAGCCAAGCGATAAGCGGTTCCGGCTGTGGTACGTTGGGGGGTCGTGCCTG
  GACCACAGGACCACGCTGCCTATGCTGCCCTGGCTCATGGCCGAGATCCGCAGGCGCAGCCAGAAGCCC
  GAGGCGGGCGGCTGCGGGGCGCCGGCGGCCCGAGAGGTGATCCTGGTGCTCAGCGCGCCCTTCCTGCGT
  TGCGTCCCCGCGCCGGGCGCTGGGGCCTCGGGGGGCACTAGTCCGTCGGCCACGCAGCCCAACCCGGCG
  GTATTCATCTTCGAGCACAAGGCGCAGCATATCTCGCGCTTCATCCACAACAGCCACGACCTCACCTAC
  TTTGCCTACCTGATCAAGGCGCAGCCCGACGACCCCGAGTCGCAGATGGCCTGCCACGTTTTCCGCGCC
  ACAGACCCCAGCCAGGTTCCTGATGTTATTAGCAGCATAAGGCAATTATCTAAAGCGGCCATGAAAGAG
  GATGCCAAACCCAGCAAAGATAATGAGGACGCCTTTTACAACTCTCAGAAGTTCGAAGTCCTGTACTGT
  GGAAAGGTGACCGTGACCCACAAGAAGGCCCCCTCAAGCCTCATCGATGACTGCATGGAGAAGTTCAGC
  CTGCACGAACAGCAGCGCCTGAAGATCCAAGGGGAGCAGCGCGGTCCGGACCCAGGAGAGGACCTGGCT
  GACTTGGAGGTGGTGGTGCCCGGGTCCCCCGGAGACTGCCTGCCGGAGGAGGCTGACGGCACCGACACC
  CACCTTGGCTTACCTGCCGGGGCCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTGCTTCCCTGAGCGG
  ATTTTGGAAGATTCTGGCTTTGATGAGCAGCAGGAGTTTCGGTCTCGGTGCAGCAGTGTCACCGGCGTG
  CAACGGAGAGTTCACGAGGGCAGCCAGAAATCCCAGCCGCGACGGAGACACGCGAGCGCACCCAGTCAC
  GTCCAGCCCTCGGACTCGGAGAAGAACAGGACCATGCTCTTCCAGGTTGGGCGATTTGAGATTAACCTT
  ATCAGTCCAGACACTAAATCAGTTGTGCTAGAAAAGAATTTTAAAGATATCTCCTCTTGTTCTCAGGGT
  ATAAAGCATGTGGATCACTTTGGCTTTATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCCAGTATATT
  TGTTATGTATTCCAGTGTGCCAGCGAATCTCTGGTTGATGAGGTAATGCTGACTCTGAAACAGGCCTTC
  AGTACGGCGGCTGCCCTGCAGAGTGCCAAGACGCAGATTAAACTGTGTGAGGCCTGCCCGATGCACTCT
  TTGCATAAGCTCTGTGAAAGGATTGAAGGTCTCTACCCACCAAGAGCCAAGCTGGTGATACAGAGGCAT
  CTCTCATCACTGACAGATAATGAGCAAGCTGACATCTTTGAAAGAGTTCAGAAAATGAAGCCAGTCAGT
  GACCAGGAAGAAAATGAACTTGTGATTTTACACCTGAGGCAGCTGTGTGAAGCCAAGCAGAAGACACAC
  GTGCACATCGGGGAAGGCCCTTCTACTATTTCAAATAGTACAATCCCAGAAAATGCAACAAGCAGTGGA
  AGGTTCAAACTTGACATTCTGAAAAATAAAGCTAAGAGATCCTTAACTAGCTCCCTGGAAAATATCTTC
  TCAAGGGGAGCTAACAGAATGAGAGGTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTCCAACAGTCTT
  GCTTCAGAGAAGGACTACTCACCAGGGGATTCTCCACCAGGGACACCGCCAGCGTCCCCACCGTCCTCA
  GCTTGGCAAACGTTTCCCGAAGAGGATTCCGACTCCCCGCAGTTTCGAAGACGGGCACACACGTTCAGC
  CACCCACCTTCAAGCACAAAGAGAAAGCTGAATTTGCAGGATGGGAGGGCTCAGGGTGTGCGTTCCCCT
  CTGCTGAGGCAGAGCTCCAGTGAACAGTGCAGCAATCTTTCGTCAGTTCGACGCATGTACAAGGAGAGT
  AATTCTTCCTCCAGTCTTCCAAGTCTTCACACTTCCTTCTCTGCCCCTTCCTTCACTGCCCCCTCTTTC
  CTGAAAAGCTTTTACCAGAATTCAGGTAGACTGTCCCCACAGTATGAAAATGAAATCAGACAAGACACT
  GCTTCAGAATCAAGTGATGGAGAAGGGAGAAAAAGGACCTCATCTACCTGCAGCAATGAGTCCCTAAGT
  GTGGGAGGAACCTCTGTCACTCCTCGCCGGATCTCCTGGCGGCAGCGCATTTTCCTCAGGGTTGCTTCT
  CCCATGAACAAATCTCCCTCAGCAATGCAACAGCAAGATGGATTGGACAGGAACGAGCTGCTGCCACTG
  TCCCCCCTCTCTCCAACCATGGAGGAGGAACCGCTGGTTGTATTCCTGTCTGGGGAGGATGACCCAGAA
  AAGATTGAAGAAAGAAAGAAATCAAAAGAACTGAGGAGCTTGTGGAGAAAAGCTATACACCAACAAATC
  TTGTTACTTCGAATGGAAAAAGAAAACCAGAAACTTGAAGCAAGCAGAGATGAACTCCAGTCCAGAAAA
  GTTAAATTAGACTATGAAGAAGTTGGTGCATGTCAGAAAGAGGTCTTAATAACTTGGGATAAGAAGTTG
  TTAAACTGCAGAGCTAAAATCAGATGTGATATGGAAGATATTCATACTCTTCTTAAAGAAGGAGTTCCC
  AAAAGTCGACGAGGAGAAATTTGGCAGTTTCTGGCTTTACAGTACCGACTCAGACACAGATTGCCTAAT
  AAACAACAGCCTCCTGACATATCCTATAAGGAACTTTTGAAGCAGCTCACTGCTCAGCAGCATGCGATT
  CTCGTGGATTTAGGAAGGACGTTTCCTACTCACCCTTACTTTTCAGTACAGCTTGGGCCAGGACAGCTG
  TCACTGTTTAACCTCCTGAAAGCCTATTCTTTGCTGGACAAAGAAGTGGGATACTGTCAGGGGATCAGC
  TTTGTGGCTGGAGTCCTGCTTCTGCACATGAGTGAAGAGCAAGCCTTTGAAATGCTGAAATTCCTCATG
  TATGACCTCGGCTTCCGCAAGCAGTACAGACCTGACATGATGTCGCTGCAGATTCAAATGTACCAGCTG
  TCCAGGCTCCTTCATGACTATCACAGAGATCTCTACAATCACCTTGAAGAAAATGAAATCAGCCCCAGT
  CTTTATGCTGCCCCCTGGTTCCTCACATTGTTTGCCTCTCAGTTTTCATTAGGATTTGTAGCCAGAGTT
  TTTGATATTATTTTTCTTCAGGGAACTGAAGTTATATTCAAGGTTGCACTCAGCCTACTGAGCAGCCAA
  GAGACACTTATAATGGAATGTGAGAGCTTTGAAAATATTGTTGAGTTTCTTAAAAACACGCTACCTGAT
  ATGAATACCTCTGAAATGGAAAAAATTATTACCCAGGTTTTTGAGATGGATATTTCTAAGCAGTTGCAT
  GCCTATGAGGTGGAATATCATGTGCTACAGGATGAGCTTCAGGAATCTTCATATTCCTGTGAGGATAGT
  GAAACTTTGGAGAAGCTGGAGAGGGCCAATAGCCAACTGAAAAGACAAAACATGGACCTCCTAGAAAAA
  TTACAGGTAGCTCATACTAAAATCCAGGCCTTGGAATCAAACCTGGAAAATCTTTTGACGAGAGAGACC
  AAAATGAAGTCTTTAATCCGGACCCTGGAACAAGAAAAAATGGCTTATCAAAAGACAGTGGAGCAACTC
  CGGAAGCTGCTGCCCGCGGATGCTCTAGTCAATTGTGACCTGTTGCTGAGAGACCTAAACTGCAACCCT
  AACAACAAAGCCAAGATAGGAAATAAGCCATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014832
• Insert Size: 3897 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014832.4
• RefSeq Size: 6443 bp
• RefSeq ORF: 3897 bp
• Locus ID: 9882
• UniProt ID: O60343
• Cytogenetics: 13q22.2
• Domains: TBC, PID
• MW: 146.6 kDa
• Gene Summary: This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.