Cytochrome b5 (CYB5A) (NM_148923) Human Untagged Clone

SKU SC309790

CYB5A (untagged)-Human cytochrome b5 type A (microsomal) (CYB5A), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Cytochrome b5
• Synonyms: CYB5; MCB5; METAG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_148923 edited; CCTCGTGCCGAATTCGGCACGAGGGGGGAATGTCCCGGGTGGAGCTGGCTGAGTCGCGCG; CTCTGCTCCACCCGACGGGGCTGTGTGTGCTGGGCCTGGCTCGCGGCGAACCGAGATGGC; AGAGCAGTCGGACGAGGCCGTGAAGTACTACACCCTAGAGGAGATTCAGAAGCACAACCA; CAGCAAGAGCACCTGGCTGATCCTGCACCACAAGGTGTACGATTTGACCAAATTTCTGGA; AGAGCATCCTGGTGGGGAAGAAGTTTTAAGGGAACAAGCTGGAGGTGACGCTACTGAGAA; CTTTGAGGATGTCGGGCACTCTACAGATGCCAGGGAAATGTCCAAAACATTCATCATTGG; GGAGCTCCATCCAGATGACAGACCAAAGTTAAACAAGCCTCCGGAAACTCTTATCACTAC; TATTGATTCTAGTTCCAGTTGGTGGACCAACTGGGTGATCCCTGCCATCTCTGCAGTGGC; CGTCGCCTTGATGTATCGCCTATACATGGCAGAGGACTGAACACCTCCTCAGAAGTCAGC; GCAGGAAGAGCCTGCTTTGGACACGGGAGAAAAGAAGCCATTGCTAACTACTTCAACTGA; CAGAAACCTTCACTTGAAAACAATGATTTTAATATATCTCTTTCTTTTTCTTCCGACATT; AGAAACAAAACAAAAAGAACTGTCCTTTCTGCGCTCAAATTTTTCGAGTGTGCCTTTTTA; TTCATCTACTTTATTTTGATGTTTCCTTAATGTGTAATTTACTTATTATAAGCATGATCT; TTTAAAAATATATTTGGCTTTTAAAGTAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_148923 unedited; ACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGG; GGGGAATGTCCCGGGTGGAGCTGGCTGAGTCGCGCGCTCTGCTCCACCCGACGGGGCTGT; GTGTGCTGGGCCTGGCTCGCGGCGAACCGAGATGGCAGAGCAGTCGGACGAGGCCGTGAA; GTACTACACCCTAGAGGAGATTCAGAAGCACAACCACAGCAAGAGCACCTGGCTGATCCT; GCACCACAAGGTGTACGATTTGACCAAATTTCTGGAAGAGCATCCTGGTGGGGAAGAAGT; TTTAAGGGAACAAGCTGGAGGTGACGCTACTGAGAACTTTGAGGATGTCGGGCACTCTAC; AGATGCCAGGGAAATGTCCAAAACATTCATCATTGGGGAGCTCCATCCAGATGACAGACC; AAAGTTAAACAAGCCTCCGGAAACTCTTATCACTACTATTGATTCTAGTTCCAGTTGGTG; GACCAACTGGGTGATCCCTGCCATCTCTGCAGTGGCCGTCGCCTTGATGTATCGCCTATA; CATGGCAGAGGACTGAACACCTCCTCAGAAGTCAGCGCAGGAAGAGCCTGCTTTGGACAC; GGGAGAAAAGAAGCCATTGCTAACTACTTCAACTGACAGAAACCTTCACTTGAAAACAAT; GATTNTAATATATCTCTTTCTTTNTCTTCCGACATTAGAAACAAAACAAAAAGAACTGTC; CTTTCTGCGCTCAAATTTTTCGAGTGTGCCTTTTTATTCATCTACTTTATTNTGATGGTT; CCTTTATGGNNGTATTACTTTTATATAGCATGATCTTTTAAAATATATTTGGCTTTTAAG; TANAAAAAAAAAAAAACTCGCTCTAGATTGGGNCGCGTCATAGCTGTTTCCTGACAGACC; GGNNTGCATCCCTGTGACCCTNCCCAGTGCTTCCTGGCCTGNNAGTGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_148923 unedited; CCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTACTTTAAAAGCCAAATATATTTTT; AAAAGATCATGCTTATAATAAGTAAATTACACATTAAGGAAACATCAAAATAAAGTAGAT; GAATAAAAAGGCACACTTCGAAAAATTTGAGCGCAGAAAGGACAGTTCTTTTTGTTTTGT; TTCTAATGTCGGAAGAAAAAGAAAGAGATATATTAAAATCATTGTTTTCAAGTGAAGGTT; TCTGTCAGTTGAAGTAGTTAGCAATGGCTTCTTTTCTCCCGTGTCCAAAGCAGGCTCTTC; CTGCGCTGACTTCTGAGGAGGTGTTCAGTCCTCTGCCATGTATAGGCGATACATCAAGGC; GACGGCCACTGCAGAGATGGCAGGGATCACCCAGTTGGTCCACCAACTGGAACTAGAATC; AATAGTAGTGATAAGAGTTTCCGGAGGCTTGTTTAACTTTGGTCTGTCATCTGGATGGAG; CTCCCCAATGATGAATGTTTTGGACATTTCCCTGGCATCTGTAGAGTGCCCGACATCCTC; AAAGTTCTCAGTAGCGTCACCTCCAGCTTGTTCCCTTAAAACTTCTTCCCCACCAGGATG; CTCTTCCAGAAATTTGGTCAAATCGTACACCTTGTGGTGCANGATCAGCCAGGTGCTCTT; GCTGTGGTTGTGCTTCTGAAATCTCTCTAGGNTGTAGTACTTCACGGNCTCGTNCGACTG; CTCTTGCATCTCGGGGTTCGCGCGAGCCANGCCCCAGACACACAGCCCNGTNNNGGTGGG; GAGCANAAGGGCGCGACTTAACCAGNTTTCCACCCGGGACATTCCCCCTCGTGCCGAATT; CGGGNCGAGGGCTTGTGCCGAATTCCGGGGCGCCCTTAAGGGGATTCGTATTACAAATTT; TGGACGGTCACTAAACAAACTTGCTAAAATTAACTTTCACGGTACAGCCTACCGCCCATT; GGTAACGGGGGGGGGGGTATATACAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_148923
• Insert Size: 900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_148923.2, NP_683725.1
• RefSeq Size: 820 bp
• RefSeq ORF: 405 bp
• Locus ID: 1528
• UniProt ID: P00167
• Cytogenetics: 18q22.3
• Protein Families: Transmembrane
• Summary: The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).