EPHA8 (NM_020526) Human Untagged Clone

SKU SC309717

EPHA8 (untagged)-Human EPH receptor A8 (EPHA8), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: EPHA8
• Synonyms: EEK; EK3; HEK3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC309717 representing NM_020526.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCCCCCGCCCGGGGCCGCCTGCCCCCTGCGCTCTGGGTCGTCACGGCCGCGGCGGCGGCGGCCACC; TGCGTGTCCGCGGCGCGCGGCGAAGTGAATTTGCTGGACACGTCGACCATCCACGGGGACTGGGGCTGG; CTCACGTATCCGGCTCATGGGTGGGACTCCATCAACGAGGTGGACGAGTCCTTCCAGCCCATCCACACG; TACCAGGTTTGCAACGTCATGAGCCCCAACCAGAACAACTGGCTGCGCACGAGCTGGGTCCCCCGAGAC; GGCGCCCGGCGCGTCTATGCTGAGATCAAGTTTACCCTGCGCGACTGCAACAGCATGCCTGGTGTGCTG; GGCACCTGCAAGGAGACCTTCAACCTCTACTACCTGGAGTCGGACCGCGACCTGGGGGCCAGCACACAA; GAAAGCCAGTTCCTCAAAATCGACACCATTGCGGCCGACGAGAGCTTCACAGGTGCCGACCTTGGTGTG; CGGCGTCTCAAGCTCAACACGGAGGTGCGCAGTGTGGGTCCCCTCAGCAAGCGCGGCTTCTACCTGGCC; TTCCAGGACATAGGTGCCTGCCTGGCCATCCTCTCTCTCCGCATCTACTATAAGAAGTGCCCTGCCATG; GTGCGCAATCTGGCTGCCTTCTCGGAGGCAGTGACGGGGGCCGACTCGTCCTCACTGGTGGAGGTGAGG; GGCCAGTGCGTGCGGCACTCAGAGGAGCGGGACACACCCAAGATGTACTGCAGCGCGGAGGGCGAGTGG; CTCGTGCCCATCGGCAAATGCGTGTGCAGTGCCGGCTACGAGGAGCGGCGGGATGCCTGTGTGGCCTGT; GAGCTGGGCTTCTACAAGTCAGCCCCTGGGGACCAGCTGTGTGCCCGCTGCCCTCCCCACAGCCACTCC; GCAGCTCCAGCCGCCCAAGCCTGCCACTGTGACCTCAGCTACTACCGTGCAGCCCTGGACCCGCCGTCC; TCAGCCTGCACCCGGCCACCCTCGGCACCAGTGAACCTGATCTCCAGTGTGAATGGGACATCAGTGACT; CTGGAGTGGGCCCCTCCCCTGGACCCAGGTGGCCGCAGTGACATCACCTACAATGCCGTGTGCCGCCGC; TGCCCCTGGGCACTGAGCCGCTGCGAGGCATGTGGGAGCGGCACCCGCTTTGTGCCCCAGCAGACAAGC; CTGGTGCAGGCCAGCCTGCTGGTGGCCAACCTGCTGGCCCACATGAACTACTCCTTCTGGATCGAGGCC; GTCAATGGCGTGTCCGACCTGAGCCCCGAGCCCCGCCGGGCCGCTGTGGTCAACATCACCACGAACCAG; GCAGCCCCGTCCCAGGTGGTGGTGATCCGTCAAGAGCGGGCGGGGCAGACCAGCGTCTCGCTGCTGTGG; CAGGAGCCCGAGCAGCCGAACGGCATCATCCTGGAGTATGAGATCAAGTACTACGAGAAGGACAAGGAG; ATGCAGAGCTACTCCACCCTCAAGGCCGTCACCACCAGAGCCACCGTCTCCGGCCTCAAGCCGGGCACC; CGCTACGTGTTCCAGGTCCGAGCCCGCACCTCAGCAGGCTGTGGCCGCTTCAGCCAGGCCATGGAGGTG; GAGACCGGGAAACCCCGGCCCCGCTATGACACCAGGACCATTGTCTGGATCTGCCTGACGCTCATCACG; GGCCTGGTGGTGCTTCTGCTCCTGCTCATCTGCAAGAAGAGGCACTGTGGCTACAGCAAGGCCTTCCAG; GACTCGGACGAGGAGAAGATGCACTATCAGAATGGACAGGCACCCCCACCTGTCTTCCTGCCTCTGCAT; CACCCCCCGGGAAAGCTCCCAGAGCCCCAGTTCTATGCGGAACCCCACACCTACGAGGAGCCAGGCCGG; GCGGGCCGCAGTTTCACTCGGGAGATCGAGGCCTCTAGGATCCACATCGAGAAAATCATCGGCTCTGGA; GACTCCGGGGAAGTCTGCTACGGGAGGCTGCGGGTGCCAGGGCAGCGGGATGTGCCCGTGGCCATCAAG; GCCCTCAAAGCCGGCTACACGGAGAGACAGAGGCGGGACTTCCTGAGCGAGGCGTCCATCATGGGGCAA; TTCGACCATCCCAACATCATCCGCCTCGAGGGTGTCGTCACCCGTGGCCGCCTGGCAATGATTGTGACT; GAGTACATGGAGAACGGCTCTCTGGACACCTTCCTGAGGACCCACGACGGGCAGTTCACCATCATGCAG; CTGGTGGGCATGCTGAGAGGAGTGGGTGCCGGCATGCGCTACCTCTCAGACCTGGGCTATGTCCACCGA; GACCTGGCCGCCCGCAACGTCCTGGTTGACAGCAACCTGGTCTGCAAGGTGTCTGACTTCGGGCTCTCA; CGGGTGCTGGAGGACGACCCGGATGCTGCCTACACCACCACGGGCGGGAAGATCCCCATCCGCTGGACG; GCCCCAGAGGCCATCGCCTTCCGCACCTTCTCCTCGGCCAGCGACGTGTGGAGCTTCGGCGTGGTCATG; TGGGAGGTGCTGGCCTATGGGGAGCGGCCCTACTGGAACATGACCAACCGGGATGTCATCAGCTCTGTG; GAGGAGGGGTACCGCCTGCCCGCACCCATGGGCTGCCCCCACGCCCTGCACCAGCTCATGCTCGACTGT; TGGCACAAGGACCGGGCGCAGCGGCCTCGCTTCTCCCAGATTGTCAGTGTCCTCGATGCGCTCATCCGC; AGCCCTGAGAGTCTCAGGGCCACCGCCACAGTCAGCAGGTGCCCACCCCCTGCCTTCGTCCGGAGCTGC; TTTGACCTCCGAGGGGGCAGCGGTGGCGGTGGGGGCCTCACCGTGGGGGACTGGCTGGACTCCATCCGC; ATGGGCCGGTACCGAGACCACTTCGCTGCGGGCGGATACTCCTCTCTGGGCATGGTGCTACGCATGAAC; GCCCAGGACGTGCGCGCCCTGGGCATCACCCTCATGGGCCACCAGAAGAAGATCCTGGGCAGCATTCAG; ACCATGCGGGCCCAGCTGACCAGCACCCAGGGGCCCCGCCGGCACCTCTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_020526
• Insert Size: 3018 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020526.3
• RefSeq Size: 4996 bp
• RefSeq ORF: 3018 bp
• Locus ID: 2046
• UniProt ID: P29322
• Cytogenetics: 1p36.12
• Domains: EPH_lbd, FN3, pkinase, SAM, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance
• MW: 111 kDa
• Summary: This gene encodes a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. The protein encoded by this gene functions as a receptor for ephrin A2, A3 and A5 and plays a role in short-range contact-mediated axonal guidance during development of the mammalian nervous system. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.