FGD6 (NM_018351) Human Untagged Clone

CAT#: SC308862

FGD6 (untagged)-Human FYVE, RhoGEF and PH domain containing 6 (FGD6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FGD6
• Synonyms: ZFYVE24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308862 representing NM_018351.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACTTCTGCAGCCGAGATAAAGAAGCCACCAGTGGCCCCCAAGCCCAAGTTTGTTGTGGCAAATAAT
  AAGCCAGCCCCACCTCCTATTGCACCTAAACCCGACATTGTGATTTCTAGTGTTCCACAGTCGACAAAG
  AAAATGAAACCAGCAATAGCCCCAAAACCAAAAGTCCTGAAGACCTCACCTGTTCGAGAGATTGGGCAG
  TCGCCATCAAGGAAAATCATGTTGAACCTGGAAGGGCATAAACAGGAATTAGCTGAAAGCACTGACAAC
  TTTAATTGTAAATATGAAGGCAATCAGAGCAATGATTATATTTCACCAATGTGTTCCTGCAGTTCTGAG
  TGTATCCATAAGCTGGGCCATAGAGAGAATTTGTGTGTAAAGCAGCTTGTTTTAGAGCCCCTGGAAATG
  AATGAAAATTTAGAAAACAGTAAAATTGATGAGACTTTGACTATAAAAACTAGGAGTAAATGTGATTTG
  TATGGTGAAAAAGCCAAGAACCAGGGTGGGGTTGTTTTAAAGGCAAGCGTTTTAGAAGAGGAGCTCAAA
  GATGCCTTAATACACCAAATGCCACCTTTTATTTCTGCACAGAAGCACAGGCCCACAGACAGCCCAGAA
  ATGAATGGTGGCTGTAATTCAAATGGACAATTCAGAATTGAATTTGCGGATTTGTCACCTTCCCCATCC
  AGCTTTGAAAAAGTTCCTGATCATCACAGTTGCCACTTACAGCTTCCTAGTGATGAATGTGAACATTTT
  GAAACTTGCCAGGATGACAGTGAAAAAAGCAATAATTGCTTTCAGTCATCTGAACTAGAGGCTCTGGAA
  AATGGGAAAAGGAGTACTTTAATATCTTCAGATGGAGTTAGTAAGAAATCAGAAGTCAAAGACCTTGGT
  CCCTTAGAAATTCATTTAGTACCATATACCCCAAAATTTCCAACTCCCAAGCCCAGAAAGACACGAACT
  GCTCGTCTGTTACGCCAAAAGTGTGTAGATACTCCTAGTGAAAGCACTGAAGAACCGGGGAATTCAGAC
  AGTAGCTCTTCCTGTCTTACTGAAAATAGTTTGAAAATCAATAAAATCAGTGTTCTGCATCAGAATGTT
  TTGTGTAAGCAGGAACAGGTGGATAAAATGAAGCTAGGAAATAAAAGTGAATTGAATATGGAATCCAAC
  AGTGATGCACAGGACTTAGTCAATTCACAGAAAGCCATGTGTAATGAAACAACTTCCTTTGAAAAAATG
  GCACCTTCTTTTGATAAAGACTCTAATTTGAGTTCTGACAGCACAACTGTAGATGGTTCTAGTATGTCG
  CTTGCTGTGGACGAAGGGACCGGTTTTATAAGATGTACTGTATCTATGAGCCTGCCTAAGCAGCTCAAA
  TTAACTTGCAATGAACATTTGCAATCTGGGAGAAACCTGGGAGTTTCTGCCCCTCAAATGCAAAAGGAA
  TCTGTTATAAAAGAGGAAAATTCTCTACGAATTGTCCCCAAAAAACCTCAAAGACATAGCTTGCCTGCT
  ACAGGAGTGCTTAAAAAGGCTGCCTCCGAGGAGCTTTTGGAAAAAAGTTCTTATCCTTCAAGTGAAGAA
  AAAAGTTCAGAGAAGAGTCTAGAAAGAAATCACCTTCAGCATTTGTGTGCCCAAAACCGTGGTGTGTCA
  TCCTCCTTTGATATGCCTAAACGGGCTTCAGAAAAGCCAGTGTGGAAGTTACCTCATCCTATTTTACCC
  TTTTCAGGGAACCCAGAATTCTTAAAGTCTGTCACCGTATCGTCAAACAGTGAGCCTTCAACAGCCCTA
  ACCAAGCCCAGAGCAAAATCGTTATCTGCTATGGATGTGGAAAAGTGCACTAAGCCTTGCAAAGACTCT
  ACAAAGAAAAACTCTTTTAAAAAGTTGCTCAGCATGAAACTGTCCATCTGTTTCATGAAGAGTGACTTT
  CAAAAATTTTGGTCCAAGAGTAGCCAACTCGGAGACACCACCACAGGCCACCTCTCCAGTGGGGAGCAG
  AAGGGGATTGAAAGTGATTGGCAAGGCTTGTTGGTAGGAGAGGAGAAGAGAAGTAAACCCATCAAGGCA
  TATTCCACAGAAAACTATAGCCTGGAATCTCAAAAGAAGAGGAAGAAGTCTCGGGGCCAGACCAGTGCA
  GCTAATGGTCTGAGAGCTGAGTCTTTGGATGACCAAATGCTCTCCCGGGAGTCATCATCTCAGGCACCT
  TACAAGTCTGTTACAAGCCTCTGTGCACCGGAGTATGAAAATATACGCCATTATGAGGAAATACCAGAG
  TACGAGAACTTGCCATTTATTATGGCTATACGGAAAACTCAAGAGTTGGAATGGCAGAATTCCAGCAGC
  ATGGAGGACGCTGATGCAAATGTGTATGAGGTAGAAGAGCCGTATGAAGCTCCAGATGGCCAGCTGCAG
  CTTGGACCCAGACATCAGCATTCCAGTTCAGGAGCATCCCAGGAGGAACAGAATGATCTTGGTCTTGGT
  GACCTTCCCTCTGATGAGGAGGAAATCATCAACAGTTCTGATGAAGATGATGTCAGCTCTGAGTCAAGT
  AAAGGAGAGCCTGACCCACTGGAAGATAAACAGGATGAAGATAATGGAATGAAAAGTAAAGTTCATCAT
  ATTGCCAAGGAGATCATGAGCTCAGAGAAAGTGTTTGTGGATGTGTTAAAACTTTTGCATATTGATTTC
  CGGGATGCAGTAGCTCATGCTTCCAGGCAACTTGGGAAACCAGTGATTGAGGACCGGATTCTAAATCAG
  ATCCTATACTACTTGCCTCAGCTGTATGAGCTCAACCGGGATCTCTTGAAGGAACTGGAGGAAAGAATG
  TTGCACTGGACTGAACAACAAAGAATTGCTGATATCTTTGTAAAGAAGGGACCATATCTAAAAATGTAT
  TCCACATACATCAAAGAATTTGATAAGAATATAGCCTTGCTGGATGAACAGTGCAAGAAAAATCCAGGT
  TTTGCTGCTGTTGTTAGAGAATTTGAGATGAGCCCTCGCTGTGCTAATCTGGCCCTCAAGCACTACCTG
  CTCAAGCCGGTTCAGAGGATCCCCCAGTACAGGCTGTTGCTGACAGATTATTTGAAGAATCTCATAGAA
  GATGCTGGAGATTACAGAGACACTCAAGATGCCCTTGCTGTTGTTATAGAGGTAGCCAACCACGCCAAT
  GACACCATGAAGCAAGGAGACAACTTTCAGAAACTTATGCAAATTCAGTACAGCTTAAATGGACACCAT
  GAAATTGTGCAGCCTGGTCGGGTTTTTCTCAAAGAAGGAATTCTGATGAAGCTGTCTCGGAAAGTGATG
  CAACCTCGAATGTTTTTCCTGTTTAATGATGCCCTGCTGTATACAACACCAGTGCAGTCTGGGATGTAT
  AAACTGAACAACATGCTCTCACTGGCTGGAATGAAGGTCAGAAAACCTACCCAAGAAGCCTATCAGAAT
  GAATTAAAGATTGAAAGTGTAGAACGTTCCTTCATTCTCTCAGCCAGTTCTGCCACAGAAAGGGATGAA
  TGGCTAGAAGCGATTTCCAGGGCAATAGAAGAGTATGCCAAGAAAAGAATCACCTTCTGTCCTAGTAGG
  AGTCTTGATGAGGCAGACTCAGAAAATAAAGAAGAAGTTAGTCCTCTTGGATCGAAGGCTCCCATCTGG
  ATTCCTGATACCAGAGCCACAATGTGTATGATCTGCACAAGCGAATTCACTCTCACCTGGAGACGACAC
  CACTGCCGGGCCTGTGGAAAGATTGTATGCCAAGCTTGTTCGTCTAATAAGTATGGCTTAGATTACCTG
  AAAAATCAACCAGCAAGAGTATGTGAACATTGTTTCCAAGAACTGCAGAAATTAGATCACCAGCACTCC
  CCTAGGATTGGATCTCCTGGAAATCACAAATCTCCTTCAAGTGCCTTATCATCAGTCTTACATAGCATT
  CCATCAGGGAGGAAACAGAAAAAAATCCCAGCTGCTCTCAAAGAAGTATCAGCAAACACAGAGGATTCT
  TCTATGAGTGGCTACTTGTACAGATCAAAGGGCAATAAAAAACCCTGGAAACACTTTTGGTTTGTCATA
  AAAAATAAAGTACTATATACATATGCTGCAAGTGAGGACGTGGCCGCTTTGGAGAGTCAGCCTTTATTA
  GGATTCACTGTTATTCAAGTTAAAGATGAGAATTCCGAGTCTAAAGTATTTCAGTTACTGCACAAAAAC
  ATGTTATTTTATGTATTCAAAGCAGAGGATGCTCATTCGGCTCAGAAGTGGATAGAAGCATTTCAGGAA
  GGCACAATATTGTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_018351
• Insert Size: 4293 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018351.3
• RefSeq Size: 9288 bp
• RefSeq ORF: 4293 bp
• Locus ID: 55785
• UniProt ID: Q6ZV73
• Cytogenetics: 12q22
• Domains: FYVE, PH
• MW: 160.8 kDa
• Gene Summary: May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. May play a role in regulating the actin cytoskeleton and cell shape (By similarity).[UniProtKB/Swiss-Prot Function]