FGD6 (NM_018351) Human Tagged ORF Clone

CAT#: RC213023

FGD6 (Myc-DDK-tagged)-Human FYVE, RhoGEF and PH domain containing 6 (FGD6)

Product Images

Circular map for RC213023

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FGD6
• Synonyms: ZFYVE24
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213023 representing NM_018351
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCTGCAGCCGAGATAAAGAAGCCACCAGTGGCCCCCAAGCCCAAGTTTGTTGTGGCAAATAATA
  AGCCAGCCCCACCTCCTATTGCACCTAAACCCGACATTGTGATTTCTAGTGTTCCACAGTCGACAAAGAA
  AATGAAACCAGCAATAGCCCCAAAACCAAAAGTCCTGAAGACCTCACCTGTTCGAGAGATTGGGCAGTCG
  CCATCAAGGAAAATCATGTTGAACCTGGAAGGGCATAAACAGGAATTAGCTGAAAGCACTGACAACTTTA
  ATTGTAAATATGAAGGCAATCAGAGCAATGATTATATTTCACCAATGTGTTCCTGCAGTTCTGAGTGTAT
  CCATAAGCTGGGCCATAGAGAGAATTTGTGTGTAAAGCAGCTTGTTTTAGAGCCCCTGGAAATGAATGAA
  AATTTAGAAAACAGTAAAATTGATGAGACTTTGACTATAAAAACTAGGAGTAAATGTGATTTGTATGGTG
  AAAAAGCCAAGAACCAGGGTGGGGTTGTTTTAAAGGCAAGCGTTTTAGAAGAGGAGCTCAAAGATGCCTT
  AATACACCAAATGCCACCTTTTATTTCTGCACAGAAGCACAGGCCCACAGACAGCCCAGAAATGAATGGT
  GGCTGTAATTCAAATGGACAATTCAGAATTGAATTTGCGGATTTGTCACCTTCCCCATCCAGCTTTGAAA
  AAGTTCCTGATCATCACAGTTGCCACTTACAGCTTCCTAGTGATGAATGTGAACATTTTGAAACTTGCCA
  GGATGACAGTGAAAAAAGCAATAATTGCTTTCAGTCATCTGAACTAGAGGCTCTGGAAAATGGGAAAAGG
  AGTACTTTAATATCTTCAGATGGAGTTAGTAAGAAATCAGAAGTCAAAGACCTTGGTCCCTTAGAAATTC
  ATTTAGTACCATATACCCCAAAATTTCCAACTCCCAAGCCCAGAAAGACACGAACTGCTCGTCTGTTACG
  CCAAAAGTGTGTAGATACTCCTAGTGAAAGCACTGAAGAACCGGGGAATTCAGACAGTAGCTCTTCCTGT
  CTTACTGAAAATAGTTTGAAAATCAATAAAATCAGTGTTCTGCATCAGAATGTTTTGTGTAAGCAGGAAC
  AGGTGGATAAAATGAAGCTAGGAAATAAAAGTGAATTGAATATGGAATCCAACAGTGATGCACAGGACTT
  AGTCAATTCACAGAAAGCCATGTGTAATGAAACAACTTCCTTTGAAAAAATGGCACCTTCTTTTGATAAA
  GACTCTAATTTGAGTTCTGACAGCACAACTGTAGATGGTTCTAGTATGTCGCTTGCTGTGGACGAAGGGA
  CCGGTTTTATAAGATGTACTGTATCTATGAGCCTGCCTAAGCAGCTCAAATTAACTTGCAATGAACATTT
  GCAATCTGGGAGAAACCTGGGAGTTTCTGCCCCTCAAATGCAAAAGGAATCTGTTATAAAAGAGGAAAAT
  TCTCTACGAATTGTCCCCAAAAAACCTCAAAGACATAGCTTGCCTGCTACAGGAGTGCTTAAAAAGGCTG
  CCTCCGAGGAGCTTTTGGAAAAAAGTTCTTATCCTTCAAGTGAAGAAAAAAGTTCAGAGAAGAGTCTAGA
  AAGAAATCACCTTCAGCATTTGTGTGCCCAAAACCGTGGTGTGTCATCCTCCTTTGATATGCCTAAACGG
  GCTTCAGAAAAGCCAGTGTGGAAGTTACCTCATCCTATTTTACCCTTTTCAGGGAACCCAGAATTCTTAA
  AGTCTGTCACCGTATCGTCAAACAGTGAGCCTTCAACAGCCCTAACCAAGCCCAGAGCAAAATCGTTATC
  TGCTATGGATGTGGAAAAGTGCACTAAGCCTTGCAAAGACTCTACAAAGAAAAACTCTTTTAAAAAGTTG
  CTCAGCATGAAACTGTCCATCTGTTTCATGAAGAGTGACTTTCAAAAATTTTGGTCCAAGAGTAGCCAAC
  TCGGAGACACCACCACAGGCCACCTCTCCAGTGGGGAGCAGAAGGGGATTGAAAGTGATTGGCAAGGCTT
  GTTGGTAGGAGAGGAGAAGAGAAGTAAACCCATCAAGGCATATTCCACAGAAAACTATAGCCTGGAATCT
  CAAAAGAAGAGGAAGAAGTCTCGGGGCCAGACCAGTGCAGCTAATGGTCTGAGAGCTGAGTCTTTGGATG
  ACCAAATGCTCTCCCGGGAGTCATCATCTCAGGCACCTTACAAGTCTGTTACAAGCCTCTGTGCACCGGA
  GTATGAAAATATACGCCATTATGAGGAAATACCAGAGTACGAGAACTTGCCATTTATTATGGCTATACGG
  AAAACTCAAGAGTTGGAATGGCAGAATTCCAGCAGCATGGAGGACGCTGATGCAAATGTGTATGAGGTAG
  AAGAGCCGTATGAAGCTCCAGATGGCCAGCTGCAGCTTGGACCCAGACATCAGCATTCCAGTTCAGGAGC
  ATCCCAGGAGGAACAGAATGATCTTGGTCTTGGTGACCTTCCCTCTGATGAGGAGGAAATCATCAACAGT
  TCTGATGAAGATGATGTCAGCTCTGAGTCAAGTAAAGGAGAGCCTGACCCACTGGAAGATAAACAGGATG
  AAGATAATGGAATGAAAAGTAAAGTTCATCATATTGCCAAGGAGATCATGAGCTCAGAGAAAGTGTTTGT
  GGATGTGTTAAAACTTTTGCATATTGATTTCCGGGATGCAGTAGCTCATGCTTCCAGGCAACTTGGGAAA
  CCAGTGATTGAGGACCGGATTCTAAATCAGATCCTATACTACTTGCCTCAGCTGTATGAGCTCAACCGGG
  ATCTCTTGAAGGAACTGGAGGAAAGAATGTTGCACTGGACTGAACAACAAAGAATTGCTGATATCTTTGT
  AAAGAAGGGACCATATCTAAAAATGTATTCCACATACATCAAAGAATTTGATAAGAATATAGCCTTGCTG
  GATGAACAGTGCAAGAAAAATCCAGGTTTTGCTGCTGTTGTTAGAGAATTTGAGATGAGCCCTCGCTGTG
  CTAATCTGGCCCTCAAGCACTACCTGCTCAAGCCGGTTCAGAGGATCCCCCAGTACAGGCTGTTGCTGAC
  AGATTATTTGAAGAATCTCATAGAAGATGCTGGAGATTACAGAGACACTCAAGATGCCCTTGCTGTTGTT
  ATAGAGGTAGCCAACCACGCCAATGACACCATGAAGCAAGGAGACAACTTTCAGAAACTTATGCAAATTC
  AGTACAGCTTAAATGGACACCATGAAATTGTGCAGCCTGGTCGGGTTTTTCTCAAAGAAGGAATTCTGAT
  GAAGCTGTCTCGGAAAGTGATGCAACCTCGAATGTTTTTCCTGTTTAATGATGCCCTGCTGTATACAACA
  CCAGTGCAGTCTGGGATGTATAAACTGAACAACATGCTCTCACTGGCTGGAATGAAGGTCAGAAAACCTA
  CCCAAGAAGCCTATCAGAATGAATTAAAGATTGAAAGTGTAGAACGTTCCTTCATTCTCTCAGCCAGTTC
  TGCCACAGAAAGGGATGAATGGCTAGAAGCGATTTCCAGGGCAATAGAAGAGTATGCCAAGAAAAGAATC
  ACCTTCTGTCCTAGTAGGAGTCTTGATGAGGCAGACTCAGAAAATAAAGAAGAAGTTAGTCCTCTTGGAT
  CGAAGGCTCCCATCTGGATTCCTGATACCAGAGCCACAATGTGTATGATCTGCACAAGCGAATTCACTCT
  CACCTGGAGACGACACCACTGCCGGGCCTGTGGAAAGATTGTATGCCAAGCTTGTTCGTCTAATAAGTAT
  GGCTTAGATTACCTGAAAAATCAACCAGCAAGAGTATGTGAACATTGTTTCCAAGAACTGCAGAAATTAG
  ATCACCAGCACTCCCCTAGGATTGGATCTCCTGGAAATCACAAATCTCCTTCAAGTGCCTTATCATCAGT
  CTTACATAGCATTCCATCAGGGAGGAAACAGAAAAAAATCCCAGCTGCTCTCAAAGAAGTATCAGCAAAC
  ACAGAGGATTCTTCTATGAGTGGCTACTTGTACAGATCAAAGGGCAATAAAAAACCCTGGAAACACTTTT
  GGTTTGTCATAAAAAATAAAGTACTATATACATATGCTGCAAGTGAGGACGTGGCCGCTTTGGAGAGTCA
  GCCTTTATTAGGATTCACTGTTATTCAAGTTAAAGATGAGAATTCCGAGTCTAAAGTATTTCAGTTACTG
  CACAAAAACATGTTATTTTATGTATTCAAAGCAGAGGATGCTCATTCGGCTCAGAAGTGGATAGAAGCAT
  TTCAGGAAGGCACAATATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213023 representing NM_018351
  Red=Cloning site Green=Tags(s)
  
  MTSAAEIKKPPVAPKPKFVVANNKPAPPPIAPKPDIVISSVPQSTKKMKPAIAPKPKVLKTSPVREIGQS
  PSRKIMLNLEGHKQELAESTDNFNCKYEGNQSNDYISPMCSCSSECIHKLGHRENLCVKQLVLEPLEMNE
  NLENSKIDETLTIKTRSKCDLYGEKAKNQGGVVLKASVLEEELKDALIHQMPPFISAQKHRPTDSPEMNG
  GCNSNGQFRIEFADLSPSPSSFEKVPDHHSCHLQLPSDECEHFETCQDDSEKSNNCFQSSELEALENGKR
  STLISSDGVSKKSEVKDLGPLEIHLVPYTPKFPTPKPRKTRTARLLRQKCVDTPSESTEEPGNSDSSSSC
  LTENSLKINKISVLHQNVLCKQEQVDKMKLGNKSELNMESNSDAQDLVNSQKAMCNETTSFEKMAPSFDK
  DSNLSSDSTTVDGSSMSLAVDEGTGFIRCTVSMSLPKQLKLTCNEHLQSGRNLGVSAPQMQKESVIKEEN
  SLRIVPKKPQRHSLPATGVLKKAASEELLEKSSYPSSEEKSSEKSLERNHLQHLCAQNRGVSSSFDMPKR
  ASEKPVWKLPHPILPFSGNPEFLKSVTVSSNSEPSTALTKPRAKSLSAMDVEKCTKPCKDSTKKNSFKKL
  LSMKLSICFMKSDFQKFWSKSSQLGDTTTGHLSSGEQKGIESDWQGLLVGEEKRSKPIKAYSTENYSLES
  QKKRKKSRGQTSAANGLRAESLDDQMLSRESSSQAPYKSVTSLCAPEYENIRHYEEIPEYENLPFIMAIR
  KTQELEWQNSSSMEDADANVYEVEEPYEAPDGQLQLGPRHQHSSSGASQEEQNDLGLGDLPSDEEEIINS
  SDEDDVSSESSKGEPDPLEDKQDEDNGMKSKVHHIAKEIMSSEKVFVDVLKLLHIDFRDAVAHASRQLGK
  PVIEDRILNQILYYLPQLYELNRDLLKELEERMLHWTEQQRIADIFVKKGPYLKMYSTYIKEFDKNIALL
  DEQCKKNPGFAAVVREFEMSPRCANLALKHYLLKPVQRIPQYRLLLTDYLKNLIEDAGDYRDTQDALAVV
  IEVANHANDTMKQGDNFQKLMQIQYSLNGHHEIVQPGRVFLKEGILMKLSRKVMQPRMFFLFNDALLYTT
  PVQSGMYKLNNMLSLAGMKVRKPTQEAYQNELKIESVERSFILSASSATERDEWLEAISRAIEEYAKKRI
  TFCPSRSLDEADSENKEEVSPLGSKAPIWIPDTRATMCMICTSEFTLTWRRHHCRACGKIVCQACSSNKY
  GLDYLKNQPARVCEHCFQELQKLDHQHSPRIGSPGNHKSPSSALSSVLHSIPSGRKQKKIPAALKEVSAN
  TEDSSMSGYLYRSKGNKKPWKHFWFVIKNKVLYTYAASEDVAALESQPLLGFTVIQVKDENSESKVFQLL
  HKNMLFYVFKAEDAHSAQKWIEAFQEGTIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018351
• ORF Size: 4290 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018351.4
• RefSeq Size: 4996 bp
• RefSeq ORF: 4293 bp
• Locus ID: 55785
• UniProt ID: Q6ZV73
• Cytogenetics: 12q22
• Domains: FYVE, PH
• MW: 160.6 kDa
• Gene Summary: May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. May play a role in regulating the actin cytoskeleton and cell shape (By similarity).[UniProtKB/Swiss-Prot Function]