RNF90 (TRIM7) (NM_203297) Human Untagged Clone

SKU SC308108

TRIM7 (untagged)-Human tripartite motif containing 7 (TRIM7), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: RNF90
• Synonyms: GNIP; RNF90
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308108 representing NM_203297.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGACTCAGGCCACTGGCCAAATGTTATGTCTCCATGTTCAAGTCCCTCTCCAACTTCTCCTCCTGGGA; CAGAAACAGATGGCAGCGGAGCAGGAGAAGGTGGGGGCAGAGTTCCAGGCACTGAGGGCTTTCCTGGTG; GAGCAGGAGGGTCGGCTGCTAGGCCGCCTGGAGGAACTGTCCCGGGAGGTGGCACAGAAGCAGAATGAG; AACCTGGCCCAGCTCGGGGTTGAGATCACCCAGCTGTCCAAGCTCAGCAGCCAGATCCAGGAGACAGCT; CAAAAGCCTGACCTTGACTTTCTCCAGGAATTCAAAAGCACGCTGAGCAGGTGTAGCAATGTGCCTGGC; CCCAAGCCAACCACAGTCTCTTCTGAGATGAAGAATAAAGTCTGGAATGTTTCTCTCAAGACCTTTGTC; TTAAAAGGGATGCTGAAGAAGTTCAAAGAGGACCTTCGGGGAGAGCTGGAGAAAGAGGAGAAAGTGGAG; CTCACCTTGGATCCCGACACGGCCAACCCGCGCCTCATCCTCTCTCTGGATCTTAAGGGCGTGCGCCTC; GGCGAGCGGGCCCAGGACCTGCCCAACCACCCCTGCCGCTTCGACACCAACACCCGCGTCCTGGCGTCC; TGCGGCTTCTCCTCGGGCCGGCATCACTGGGAGGTGGAGGTGGGCTCTAAGGACGGCTGGGCCTTTGGC; GTGGCCCGCGAGAGCGTGCGCCGAAAGGGCCTGACGCCCTTCACTCCCGAGGAGGGCGTCTGGGCCCTG; CAGCTCAACGGCGGCCAGTACTGGGCCGTGACCAGCCCCGAGCGGTCGCCCCTCAGCTGCGGGCACCTG; TCGCGCGTGCGGGTGGCCCTGGACCTGGAGGTGGGAGCCGTGTCCTTCTACGCTGTGGAGGACATGCGC; CACCTCTACACCTTCCGCGTCAACTTCCAGGAGCGCGTGTTCCCGCTTTTCTCTGTTTGCTCCACGGGC; ACCTACTTGCGAATCTGGCCTTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_203297
• Insert Size: 990 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_203297.1
• RefSeq Size: 3026 bp
• RefSeq ORF: 990 bp
• Locus ID: 81786
• UniProt ID: Q9C029
• Cytogenetics: 5q35.3
• Protein Families: Druggable Genome
• MW: 37.1 kDa
• Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1, a B-box type 2, and a coiled-coil region. The protein localizes to both the nucleus and the cytoplasm, and may represent a participant in the initiation of glycogen synthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]; Transcript Variant: This variant (2, also known as GNIP3) differs in the 5' UTR and the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 2, which has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.