NLRP8 (NM_176811) Human Untagged Clone

CAT#: SC307068

NLRP8 (untagged)-Human NLR family, pyrin domain containing 8 (NLRP8)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NLRP8
• Synonyms: CLR19.2; NALP8; NOD16; PAN4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC307068 representing NM_176811.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGTGACGTGAATCCACCCTCTGACACCCCCATTCCCTTTTCATCCTCCTCCACTCACAGTTCTCAT
  ATTCCGCCCTGGACATTCTCTTGCTACCCCGGCTCCCCATGTGAAAATGGGGTCATGCTGTACATGAGA
  AACGTGAGCCATGAGGAGCTACAACGGTTCAAGCAGCTCTTACTGACTGAGCTCAGTACTGGCACCATG
  CCCATCACCTGGGACCAGGTCGAGACAGCCAGCTGGGCAGAGGTGGTTCATCTCTTGATAGAGCGTTTC
  CCTGGACGACGCGCTTGGGATGTGACTTCGAACATCTTTGCCATTATGAACTGTGATAAAATGTGTGTT
  GTAGTCCGCAGAGAGATAAATGCCATTCTGCCTACCTTGGAACCAGAGGACTTGAATGTGGGAGAAACA
  CAGGTGAATCTGGAGGAAGGAGAATCTGGTAAAATACGGCGGTATAAATCGAATGTGATGGAAAAGTTT
  TTCCCCATATGGGACATTACGACTTGGCCTGGAAACCAGAGGGACTTCTTCTACCAAGGTGTACACAGG
  CACGAGGAGTACTTACCATGTCTGCTTCTGCCCAAAAGACCCCAGGGTAGACAGCCCAAGACCGTGGCC
  ATACAGGGAGCTCCTGGGATCGGAAAAACAATCCTGGCCAAAAAGGTGATGTTTGAGTGGGCCAGAAAC
  AAGTTCTACGCCCACAAGCGCTGGTGTGCTTTCTACTTCCATTGCCAAGAGGTGAACCAGACGACAGAC
  CAGAGCTTCTCCGAGCTGATTGAGCAAAAGTGGCCTGGATCTCAGGACCTCGTGTCAAAGATTATGTCC
  AAACCCGACCAACTTCTGCTGCTCTTGGATGGCTTTGAGGAGCTCACATCTACCCTCATTGACAGACTG
  GAGGACCTGAGTGAAGACTGGAGGCAGAAATTGCCTGGGTCTGTCCTACTGAGCAGTTTGCTGAGCAAA
  ACGATGCTTCCAGAGGCCACGCTACTGATCATGATAAGATTTACCTCTTGGCAGACATGCAAGCCCTTG
  CTGAAATGTCCCTCTCTCGTAACCCTTCCGGGGTTTAATACGATGGAAAAAATCAAGTATTTCCAGATG
  TATTTTGGACACACAGAGGAGGGAGACCAAGTCTTGAGTTTCGCCATGGAAAACACCATTCTCTTCTCC
  ATGTGCCGGGTCCCTGTGGTTTGCTGGATGGTCTGCTCTGGTCTGAAACAGCAAATGGAGAGAGGAAAC
  AATCTCACACAGTCATGTCCAAATGCCACCTCTGTGTTCGTCCGGTATATTTCTAGCTTGTTTCCCACC
  AGAGCTGAGAACTTTTCCAGAAAGATCCACCAAGCACAACTGGAAGGTCTGTGTCACTTGGCCGCAGAC
  AGCATGTGGCACAGGAAATGGGTGTTAGGTAAAGAAGATCTTGAGGAAGCCAAGCTGGATCAGACGGGA
  GTCACCGCCTTCCTTGGCATGAGTATTCTTCGGAGAATTGCAGGTGAGGAAGACCACTATGTCTTTACC
  CTCGTGACTTTTCAGGAATTTTTTGCGGCCTTGTTTTATGTTCTCTGTTTCCCACAAAGACTCAAAAAT
  TTTCATGTGTTGAGCCACGTGAATATCCAGCGCCTGATAGCGAGTCCCAGAGGAAGCAAAAGCTATCTC
  TCTCACATGGGACTTTTCTTATTCGGTTTTCTGAACGAGGCCTGCGCTTCGGCCGTGGAACAGTCATTC
  CAATGCAAGGTGTCTTTCGGTAATAAGAGGAAACTGCTGAAAGTCATACCTCTGTTGCATAAATGTGAC
  CCACCTTCTCCGGGCAGTGGGGTCCCGCAGTTATTCTACTGTCTGCATGAAATCCGGGAGGAAGCCTTT
  GTAAGCCAAGCCCTAAATGATTATCATAAAGTTGTCTTGAGAATTGGCAACAACAAAGAAGTTCAAGTG
  TCTGCTTTTTGCCTGAAGCGGTGTCAATATTTGCATGAGGTGGAACTGACCGTCACCCTGAACTTCATG
  AACGTGTGGAAGCTCAGCTCCAGCTCCCATCCTGGCTCTGAAGCGCCAGAGAGCAATGGGCTGCATCGT
  TGGTGGCAAGACTTATGCTCTGTGTTTGCAACGAATGATAAGCTGGAAGTCCTGACTATGACCAACAGT
  GTTTTGGGGCCTCCTTTTTTGAAGGCTCTCGCGGCCGCACTGAGGCACCCTCAGTGCAAACTGCAAAAG
  CTACTCCTAAGGCGTGTGAATAGCACCATGTTGAACCAGGACTTAATCGGTGTTTTGACGGGGAACCAG
  CATCTGAGATACTTGGAAATACAACATGTGGAAGTGGAGTCCAAAGCTGTGAAGCTTCTATGCAGGGTG
  CTGAGATCCCCCCGGTGCCGTCTGCAGTGTCTCAGGTTGGAAGACTGCTTGGCCACCCCTAGAATTTGG
  ACTGATCTTGGCAATAATCTTCAAGGTAACGGGCATCTAAAGACTCTCATACTAAGAAAAAACTCCCTG
  GAGAACTGTGGGGCGTATTACCTGTCTGTGGCCCAGCTGGAGAGGCTGTCGATAGAGAACTGCAACCTT
  ACACAGCTTACTTGTGAAAGCCTTGCCTCCTGTCTCAGGCAGAGTAAGATGCTGACCCACCTGAGCTTG
  GCAGAAAACGCCTTGAAAGATGAAGGGGCCAAGCATATTTGGAATGCCCTGCCACACCTGAGATGTCCT
  CTGCAGAGGCTGGTACTGAGAAAGTGTGACTTGACCTTTAATTGCTGTCAGGATATGATCTCTGCGCTC
  TGTAAAAATAAAACCCTGAAAAGTCTTGACCTAAGTTTTAATAGCCTGAAGGATGATGGGGTGATCCTG
  CTGTGTGAGGCCCTGAAGAACCCTGACTGTACATTACAGATCCTGGAGCTGGAAAACTGCCTGTTCACC
  TCCATCTGCTGCCAGGCCATGGCTTCCATGCTCCGCAAAAACCAACATCTGAGACATCTGGACTTGAGC
  AAGAATGCGATTGGAGTCTATGGTATTCTGACCTTGTGCGAGGCCTTCTCAAGCCAAAAGAAGAGAGAA
  GAGGTCATTTTCTGTATTCCTGCCTGGACTCGAATAACTAGCTTCTCCCCAACTCCTCACCCACCCGAC
  TTCACGGGAAAAAGTGACTGCCTATCCCAGATTAATCCTTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_176811
• Insert Size: 3147 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176811.2
• RefSeq Size: 3934 bp
• RefSeq ORF: 3147 bp
• Locus ID: 126205
• UniProt ID: Q86W28
• Cytogenetics: 19q13.43
• MW: 119.4 kDa
• Gene Summary: This gene encodes a member of the nucleotide-binding oligomerization domain/ leucine rich repeat/ pyrin domain containing (NLRP) subfamily, which belongs to the Nod-like receptor family of proteins. NLRP genes play roles in the mammalian innate immune system through inflammasome formation and activation of caspases. In addition, NLRP genes have been found to function during mammalian reproduction. Consistent with a function during human preimplantation development, this gene is expressed at high levels in oocytes with decreased levels in embryos. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).