FCHO2 (NM_138782) Human Untagged Clone

SKU SC306080

FCHO2 (untagged)-Human FCH domain only 2 (FCHO2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: FCHO2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC306080 representing NM_138782.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGTCATGGCGTATTTCGTCGAGAATTTTTGGGGGGAAAAAAATAGTGGCTTTGATGTCCTCTACCAT; AATATGAAACATGGACAGATATCAACAAAAGAACTAGCAGATTTTGTAAGGGAACGAGCTACCATAGAG; GAGGCATACTCCAGGTCAATGACAAAACTAGCAAAATCTGCAAGCAATTATTCACAACTTGGAACATTT; GCACCAGTATGGGATGTATTCAAAACATCTACAGAGAAATTAGCAAATTGTCACTTGGATCTTGTTAGA; AAATTACAAGAATTAATAAAGGAAGTTCAGAAGTATGGAGAAGAACAAGTAAAGTCTCATAAAAAGACT; AAAGAAGAAGTTGCAGGAACTCTGGAAGCTGTCCAAACCATTCAGAGCATAACTCAGGCCCTCCAGAAA; TCCAAGGAAAATTACAATGCCAAGTGTGTAGAACAGGAGCGTTTGAAAAAGGAAGGAGCTACACAAAGA; GAAATAGAAAAGGCAGCTGTTAAATCTAAGAAAGCTACAGATACCTATAAACTCTATGTGGAAAAATAT; GCATTAGCAAAAGCTGATTTCGAACAGAAAATGACAGAAACAGCTCAGAAATTTCAAGATATTGAAGAA; ACTCATCTCATTCACATAAAGGAAATTATAGGATCCTTGTCAAATGCTATAAAGGAAATTCATTTGCAG; ATAGGCCAGGTCCATGAAGAATTTATAAATAACATGGCTAATACTACAGTTGAAAGTTTGATACAAAAA; TTTGCTGAGTCAAAAGGCACTGGGAAGGAAAGACCTGGCCTCATTGAATTTGAAGAATGTGACACTGCT; AGTGCAGTTGAAGGTATAAAACCAAGGAAAAGAAAGACCTTTGCTTTGCCAGGAATCATTAAAAAGGAA; AAAGATGCAGAATCTGTGGAATGTCCTGATGCAGATTCATTGAACATTCCTGATGTAGATGAAGAAGGC; TACAGTATTAAACCAGAAACAAATCAGAATGATACCAAAGAGAACCATTTCTACTCATCTAGTGATTCT; GACTCCGAAGATGAAGAACCAAAGAAGTATCGGATAGAAATTAAGCCTATGCATCCAAATAACTCACAT; CACACAATGGCTTCTTTGGATGAATTAAAAGTATCTATAGGGAATATAACACTCTCCCCAGCAATATCT; AGACACAGTCCAGTACAGATGAATCGGAATTTGTCTAATGAGGAGTTAACAAAATCAAAGCCATCTGCT; CCACCCAATGAAAAAGGAACCAGTGATTTACTTGCTTGGGACCCCCTATTTGGACCATCTCTTGATTCA; TCTTCTTCATCTTCACTAACTTCATCATCATCAGCCAGGCCCACAACTCCTCTTTCTGTAGGCACCATT; GTCCCACCTCCGAGGCCTGCTTCCAGACCAAAGCTTACTTCAGGCAAACTCAGTGGGATTAATGAAATA; CCCAGGCCATTCAGCCCACCTGTAACTTCCAACACCAGCCCACCTCCTGCTGCACCATTAGCCCGGGCA; GAAAGTTCTTCTTCTATCTCATCATCTGCTTCATTGAGTGCTGCCAATACTCCAACAGTAGGTGTGTCA; CGGGGTCCCAGCCCTGTCAGCCTTGGAAATCAGGATACCTTACCTGTGGCAGTTGCCCTTACAGAATCT; GTTAATGCCTACTTTAAAGGAGCAGATCCCACCAAGTGTATTGTGAAGATCACTGGTGATATGACAATG; TCATTTCCAAGTGGAATTATTAAAGTCTTCACCAGCAATCCAACTCCAGCTGTGTTGTGCTTCAGGGTG; AAAAATATCAGCAGACTAGAGCAGATTCTTCCAAATGCACAGCTTGTGTTCAGTGATCCATCACAATGT; GATTCCAACACAAAAGATTTTTGGATGAACATGCAAGCTGTTACAGTCTACCTCAAGAAGCTGTCAGAG; CAAAATCCAGCTGCTTCTTATTATAATGTAGATGTATTAAAGTATCAGGTATCATCAAATGGTATTCAG; TCCACTCCTCTGAATCTTGCAACATACTGGAAATGTAGTGCTAGCACCACAGATCTTAGAGTGGATTAT; AAATACAATCCAGAAGCTATGGTGGCACCTAGTGTGCTTTCCAACATACAGGTGGTGGTACCAGTGGAT; GGAGGAGTAACGAACATGCAGTCCCTTCCCCCTGCAATATGGAATGCAGAACAAATGAAAGCCTTTTGG; AAATTGTCTAGTATTTCAGAAAAATCAGAAAATGGAGGTTCTGGGTCCCTCCGAGCAAAATTTGATCTT; TCAGAAGGACCTAGTAAACCCACGACACTTGCAGTACAATTCCTCAGCGAGGGAAGTACCCTTTCAGGA; GTAGATTTCGAACTTGTGGGCACTGGCTATAGGCTTTCCTTAATAAAGAAGCGGTTTGCTACTGGACGA; TACCTGGCGGATTGTTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_138782
• Insert Size: 2433 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_138782.2
• RefSeq Size: 4994 bp
• RefSeq ORF: 2433 bp
• Locus ID: 115548
• UniProt ID: Q0JRZ9
• Cytogenetics: 5q13.2
• MW: 88.9 kDa
• Summary: Functions in an early step of clathrin-mediated endocytosis. Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a lipid-binding activity with a preference for membranes enriched in phosphatidylserine and phosphoinositides (Pi(4,5) biphosphate) like the plasma membrane. Its membrane-bending activity might be important for the subsequent action of clathrin and adaptors in the formation of clathrin-coated vesicles. Involved in adaptor protein complex AP-2-dependent endocytosis of the transferrin receptor, it also functions in the AP-2-independent endocytosis of the LDL receptor.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).