KIAA0100 (NM_014680) Human Untagged Clone

SKU SC304154

KIAA0100 (untagged)-Human KIAA0100 (KIAA0100)

Product Data

• Type: Human Untagged Clone
• Target Symbol: KIAA0100
• Synonyms: BCOX; BCOX1; CT101; FMP27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304154 representing NM_014680.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCCTCTGTTCTTCTCCGCGCTGTTGGTCTTGCTGCTAGTTGCGCTTAGCGCCCTCTTTCTAGGCCGG; TGGCTTGTGGTCCGGTTGGCCACCAAGTGGTGTCAGCGGAAGCTGCAGGCGGAGCTAAAGATTGGCTCC; TTCCGCTTTTTTTGGATCCAGAATGTCAGTCTTAAGTTTCAGCAACACCAGCAAACAGTGGAAATTGAT; AACCTGTGGATTTCCAGCAAACTCCTTAGCCATGATCTTCCACACTATGTGGCATTGTGCTTTGGAGAA; GTGCGTATCAGAACGGACCTACAGAAAGTTTCTGACCTGTCTGCCCCATTCTCCCAGAGCGCTGGGGTG; GATCAAAAGGAACTGTCCTTCAGCCCATCCTTATTGAAGATCTTCTGCCAACTATTCTCCATTCATGTA; GATGCTATAAACATCATGGTTCTCAAGGTGGATACCTCTGAGTCCTTATGGCATATTCAGATCAGTAGA; AGCAGATTTCTTTTGGATAGTGATGGGAAAAGGCTAATCTGTGAGGTGAGCTTATGTAAGATCAACAGC; AAAGTTCTAAAGAGTGGTCAGCTGGAGGACACCTGCCTAGTGGAGCTTTCACTGGCCCTGGACCTGTGT; CTAAAGGTGGGCATTAGCAGTCGGCATCTCACTGCTATCACTGTGGATGTGTGGACACTCCATGCTGAA; CTGCATGAGGGCCTCTTCCAGAGCCAACTGCTGTGCCAGGGCCCAAGCCTAGCATCTAAGCCTGTTCCC; TGTTCAGAGGTGACAGAAAACTTAGTTGAGCCAACTCTGCCTGGCCTATTCCTTCTCCAGCAGCTGCCA; GACCAGGTCAAGGTTAAGATGGAGAACACAAGCGTGGTATTGTCCATGAATAGTCAAAAGAGGCACCTG; ACTTGGACTCTGAAGCTGCTGCAGTTCCTGTACCACCGTGATGAGGATCAGCTGCCCCTTCGAAGCTTC; ACAGCAAACTCTGATATGGCACAGATGAGCACTGAACTGCTGCTGGAAGATGGGTTGTTGTTGTCCCAG; AGTCGCCAACGCATTGTCTGCCTCAACTCCCTCAAGGCTAGTGTGCAGGTGACCACCATTGACCTCTCA; GCCTCCCTAGTTCTGAACACTTGCATCATTCACTACCGGCACCAGGAATTCTCTCACTGGCTGCACCTG; CTAGCACTGGAAACCCAAGGGTCTAGTTCACCTGTTCTAAAGCAAAGGAAAAAAAGAACCTTCCCCCAA; ATCCTGGCTCCCATCATCTTTAGCACCTCCATCTCCAATGTCAACATTTCCATTCAACTTGGAGATACA; CCACCTTTTGCCTTGGGATTCAATTCTATCTCTCTGGATTACCAGCACCTCAGGCCACAAAGCATCCAT; CAGCGGGGCGTCCTAACTGTGGACCACCTCTGCTGGCGTGTGGGCAGTGACTCCCACATTCAGCGGGCG; CCACACCCACCCAATATGCATGTTTGGGGTGAGGCACTTGTTCTGGACTCCTTCACACTACAGGGTAGC; TATAACCAGCCTCTGGGCCTGTCCAGCACCCAGTCAGATACCCTTTTTCTTGATTGTACCATTCGAGGA; CTTCAGGTGGAAGCATCAGATACCTGTGCCCAATGTCTGTCTCGTATCTTATCCCTGATGGGTCCACAA; TCTGGGAAGTCAGCTGTCTCTAGGCACTCTTCATTTGGGGAATCTGTGTCATTACTGTGGAAGGTGGAC; TTGAAGGTCGAAGACATGAACTTGTTTACCCTTTCTGCCTTGGTTGGTGCTTCAGAGGTACGACTGGAC; ACCCTAACTATCCTGGGCAGTGCAGAGACGTCCACTGTGGGGATTCAAGGACTTGTGTTAGCGCTGGTG; AAATCAGTCACGGAGAAGATGCAACCCTGTTGCAAGGCCCCTGACATCCCTACCCCAGTGCTCAGCCTT; TCCATGCTCTCCATCACCTATCACAGCAGCATCCGCTCTCTGGAGGTTCAGTGTGGTGCAGGGCTGACC; TTACTTTGGAGCCCCCCAGATCACATGTACCTGTACCAGCATGTCCTGGCCACTCTACAGTGCCGAGAC; CTACTAAGAGCCACTGTGTTTCCTGAGACTGTACCATCCCTTGCACTAGAGACTTCAGGAACTACTTCT; GAGCTAGAAGGCCGTGCCCCTGAGCCATTACCCCCAAAGCGGCTGCTAAACCTAACCCTGGAGGTGAGC; ACAGCCAAGCTCACAGCTTTTGTAGCTGAGGACAAGTTCATTACCCTGGCTGCAGAGAGTGTGTCACTG; AGCCGGCATGGAGGTTCCCTGCAGGCATACTGTCCAGAGCTGGCTGCTGGCTTTGATGGCAATAGTATC; TTCAACTTCAAGGAGGTGGAGGTGCAGCTGCTACCTGAGCTGGAAGAGATGATCCTCCACCGGAACCCC; TTCCCTGCGCTGCAGACCCTCCGGAACCGTGTTTGGCTCCTCTCTTTCGGCTCAGTCTCGGTGGAGTTT; CCTTATCAGTATGACTTTTCTCGAACTCTAGATGAGGCTGTGGGAGTTCAGAAGTGGCTGAAGGGACTA; CATCAAGGGACTCGTGCTTGGGCCTCTCCAAGCCCTGTCCCACTCCCACCTGATCTACTCTTAAAGGTT; GAGCACTTCTCATGGGTTTTCTTGGATGATGTTTTTGAGGTGAAACTTCATGATAACTACGAGCTGATG; AAGGATGAAAGTAAGGAGAGTGCCAAAAGACTACAGCTACTGGATGCTAAAGTGGCCGCCCTTCGGAAG; CAGCATGGGGAGTTGTTGCCTGCCCGCAAAATTGAGGAGCTCTATGCCTCTTTGGAACGCAAAAACATT; GAAATCTACATCCAGCGTTCCCGTCGTCTCTATGGCAACACACCCATGCGCCGGGCACTGCTTACTTGG; AGCTTAGCAGGGCTAGAACTGGTAGCTCTGGCAGATGCCTCCTTCCATGGTCCTGAGCATGTGGTAGAA; CAGGTTCAAGAGCTTGATCCAGGCAGCCCTTTTCCCCCTGAGGGATTAGATCTTGTCATTCAGTGGTGT; CGAATGCTCAAGTGCAATGTCAAGAGCTTTCTGGTTCGGATCAGGGACTATCCACGGTACCTGTTTGAG; ATCCGTGACTGGCGGCTAATGGGTCGACTTGTGGGCACCGAGCAGAGTGGTCAGCCTTGCTCCCGTCGG; CGTCAGATCTTGCACTTGGGGCTTCCGTGGGGTAACGTGGCAGTGGAGAGGAACATGCCCCCACTCAAA; TTCTACCATGACTTTCACTCGGAAATATTCCAGTACACAGTGGTGTGGGGCCCATGCTGGGATCCAGCC; TGGACACTAATTGGCCAGTGTGTGGACCTCTTGACCAAGCCCTCAGCTGACCCCAGCCCACCTTTGCCC; TGGTGGGACAAGAGCCGTCTTCTGTTCCATGGAGACTGGCACATGGACATTGAACAGGCGAACCTGCAC; CAGCTGGCCACTGAGGATCCATACAACACAACTGAAAATATGCACTGGGAGTGGAGCCACCTGTCTTTT; CATTGGAAACCTGGTCAGTTTGTGTTCAAGGGTGACTTGGATATCAACGTGAGAACAGCCTCTAAGTAT; GACGACTGCTGCTTCCTTCACCTGCCTGACCTCTGCATGACACTGGACCTGCAGTGGCTGTGCCATGGG; AACCCCCATGATCACCATAGTGTCACTCTGCGGGCCCCAGAGTTCCTGCCTGAGGTGCCCTTGGGCCAG; CTTCATGACTCCTACCGGGCCTTTCGCTCGGAGAACCTCAATCTCTCCATCAAGATGGATCTGACTCGG; CACAGTGGAACAATATCCCAGCCCCGAATTCTGCTATATAGTAGTACCCTGCGCTGGATGCAAAACTTC; TGGGCAACTTGGACAAGTGTCACAAGGCCTATCTGCAGGGGAAAGCTCTTCAATAACCTGAAACCCAGC; AAGAAGAAACTTGGTCAGCACTACAAGCAACTTTCCTATACAGCCCTCTTTCCCCAGCTGCAGGTACAT; TATTGGGCCTCATTTGCCCAGCAACGGGGCATCCAGATTGAGTGCAGTCAGGGCCATGTCTTCACTCGG; GGGACTCAGCGGCTTATACCTCAAGCAGGCACAGTGATGCGGCGCCTTATCTCTGATTGGAGTGTTACC; CAGATGGTGAGTGACCTAAGTCAGGTGACCGTTCACCTGATGGCCTCACCCACTGAAGAGAATGCTGAT; CACTGTCTTGATCCCTTGGTAACAAAGACCCACCTGCTGAGCTTGTCCTCCCTCACCTACCAACGGCAT; AGCAATCGCACAGCTGAGGAGGAGCTCTCTGCTCGTGATGGGGATCCTACCTTTCATACACATCAGCTG; CACTTAGTAGATTTACGGATTTCCTGGACAACTACCAATCGAGACATTGCCTTTGGGTTATATGATGGG; TACAAAAAGGCAGCTGTACTCAAACGTAATCTTTCTACTGAGGCCCTGAAGGGGTTAAAGATTGATCCA; CAGATGCCAGCCAAAAAGCCAAAGCGGGGTGTCCCAACTAGTGCCTCAGCCCCACCTCGTGTTAACACT; CCCAGCTTCAGTGGACAACCTGATAAGGGGTCATCAGGAGGTGCTTACATGTTGCAGAAGCTAATTGAA; GAGACAGATAGGTTTGTAGTGTTCACAGAAGAGGAATCAGGCATGAGTGACCAGTTGTGTGGCATTGCT; GCCTGCCAGACGGATGACATATACAACCGAAACTGCCTTATTGAATTGGTCAACTGTCAGATGGTTCTT; CGTGGAGCAGAGACAGAAGGCTGTGTCATTGTGTCAGCTGCCAAAGCCCAACTGCTGCAGTGCCAGCAC; CATCCAGCCTGGTATGGTGATACATTGAAGCAAAAGACATCCTGGACTTGCCTCTTGGATGGCATGCAG; TACTTTGCCACCACTGAAAGCAGCCCCACAGAGCAGGATGGCCGACAGCTCTGGTTAGAGGTGAAGAAT; ATCGAGGAGCACCGGCAGCGTAGTCTGGACTCTGTGCAGGAGCTGATGGAGAGTGGGCAGGCAGTGGGC; GGCATGGTTACCACAACCACAGATTGGAACCAGCCAGCTGAGGCACAGCAAGCCCAGCAAGTCCAGCGG; ATCATTTCGCGTTGCAACTGCCGAATGTACTATATTAGTTACAGCCATGACATTGATCCTGAACTAGCA; ACTCAGATTAAGCCACCTGAAGTTCTTGAGAACCAGGAAAAGGAAGATCTCCTAAAGAAGCAGGAAGGG; GCTGTGGATACCTTCACCCTTATCCACCATGAGCTGGAAATTTCCACCAACCCAGCTCAGTATGCCATG; ATCCTGGACATTGTCAACAACCTGCTGCTCCATGTAGAACCTAAGCGGAAGGAACATAGTGAGAAGAAG; CAACGGGTCAGGTTCCAGCTTGAGATCTCTAGCAATCCAGAGGAGCAACGCAGCAGCATACTGCATTTG; CAGGAGGCTGTGCGGCAGCATGTGGCCCAAATACGACAGCTGGAGAAGCAGATGTATTCTATCATGAAG; TCTTTGCAGGATGACAGCAAGAATGAGAATCTGCTTGACCTGAACCAGAAGCTTCAGTTGCAGCTAAAC; CAGGAGAAGGCCAACCTGCAGCTGGAAAGTGAAGAACTGAATATCCTCATCAGGTGTTTTAAGGATTTC; CAACTGCAGCGGGCTAACAAGATGGAGCTGCGAAAGCAGCAAGAAGATGTGAGTGTGGTCCGTCGCACT; GAGTTTTACTTTGCTCAGGCACGGTGGCGCCTGACAGAGGAAGATGGACAGCTGGGAATTGCTGAATTA; GAACTGCAGAGGTTCCTCTACAGCAAGGTGAATAAGTCTGATGACACAGCAGAACATCTTCTGGAGTTG; GGCTGGTTTACCATGAACAACCTCCTCCCCAATGCTGTCTATAAGGTAGTACTGCGGCCCCAGAGCTCC; TGCCAGTCTGGGCGACAGCTAGCTCTCCGCCTCTTCAGCAAAGTTCGGCCCCCTGTTGGGGGTATCTCT; GTTAAGGAGCATTTTGAGGTAAATGTGGTGCCTCTCACCATCCAGCTGACACACCAGTTCTTCCACAGA; ATGATGGGCTTTTTCTTTCCTGGCCGAAGTGTGGAAGATGATGAAGTTGGTGATGAAGAGGATAAGTCC; AAACTGGTGACTACTGGAATACCAGTGGTGAAGCCTCGGCAGCTGATTGCAACAGATGATGCAGTACCA; CTGGGCCCTGGGAAGGGTGTGGCACAGGGTTTGACTCGGAGTTCTGGGGTCAGAAGGTCATTTCGCAAA; TCGCCAGAGCACCCTGTGGATGACATTGACAAGATGAAAGAGCGAGCTGCCATGAACAACTCCTTCATC; TACATAAAGATTCCACAGGTTCCACTGTGTGTCAGCTACAAGGGTGAGAAGAACAGTGTGGACTGGGGT; GACCTTAACCTGGTGCTGCCCTGTCTGGAGTACCACAACAACACATGGACATGGCTAGACTTTGCCATG; GCTGTCAAAAGGGACAGCCGCAAAGCCCTGGTTGCCCAGGTAATCAAAGAGAAGCTAAGGCTGAAGTCT; GCAACAGGCTCTGAGGTCCGGGGAAAGCTAGAAACTAAATCGGACCTGAACATGCAACAGCAGGAAGAG; GAGGAGAAAGCCCGGCTCCTCATTGGTTTAAGTGTGGGCGACAAGAACCCTGGCAAGAAGTCCATCTTT; GGCAGGCGCAAATGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_014680
• Insert Size: 6708 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014680.3
• RefSeq Size: 7452 bp
• RefSeq ORF: 6708 bp
• Locus ID: 9703
• UniProt ID: Q14667
• Cytogenetics: 17q11.2
• Protein Families: Transmembrane
• MW: 253.7 kDa
• Summary: This gene was initially characterized in human as having high expression levels in breast carcinomas and breast cancer cell lines. This gene also has increased expression in prostrate cancer cells relative to normal prostrate tissues. Expression of this gene is negatively regulated by direct binding of the microRNA miR-195 to its 3' UTR. miR-195 has been shown to modulate the invasiveness of prostrate cancer cells and xenograft metastases by downgrading expression of this gene. In mouse, the protein encoded by this gene was identified as an antigen on acute monocytic leukemia cells. In human, alternative splicing results in multiple transcript variants encoding distinct isoforms; some of these isoforms are predicted to contain an RNA pol II promoter FMP27 protein domain and a Golgi-body-localization APT1 domain. [provided by RefSeq, Apr 2017]; Transcript Variant: This variant (1) encodes the longest isoform (1).