NKX2.8 (NKX2-8) (NM_014360) Human Untagged Clone

SKU SC304088

NKX2 (untagged)-Human NK2 homeobox 8 (NKX2-8)

Product Data

• Type: Human Untagged Clone
• Target Symbol: NKX2.8
• Synonyms: Nkx2-9; NKX2.8; NKX2H
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014360 edited; ATGGCCACCTCTGGACGCCTGAGCTTCACCGTGCGCAGCCTTCTAGATTTACCCGAGCAG; GACGCGCAACACCTGCCGAGGCGGGAGCCAGAACCACGCGCCCCCCAGCCCGACCCCTGC; GCCGCCTGGCTGGATTCGGAGCGCGGCCACTACCCTTCCTCGGACGAGAGCAGCCTGGAG; ACCAGCCCGCCAGACTCGTCGCAGCGGCCGTCCGCTAGGCCCGCGTCTCCGGGCTCGGAC; GCCGAGAAAAGGAAGAAGCGGCGGGTGCTATTCTCCAAGGCGCAGACGCTGGAGTTGGAG; CGGCGCTTCCGGCAGCAGCGGTACCTGTCTGCGCCCGAGCGCGAGCAGCTGGCGAGCCTG; CTTCGCCTCACGCCCACGCAGGTCAAGATCTGGTTCCAGAATCATCGCTACAAGCTGAAG; CGCGCTCGCGCTCCAGGGGCGGCGGAGTCGCCTGACCTGGCAGCATCCGCCGAGCTGCAC; GCCGCGCCCGGCCTGCTGCGTCGCGTGGTGGTGCCGGTGCTTGTTCGCGACGGGCAGCCG; TGCGGCGGCGGCGGCGGTGGCGAGGTGGGAACCGCCGCGGCCCAGGAGAAGTGCGGCGCC; CCTCCAGCCGCCGCCTGCCCTCTGCCGGGCTACCCTGCCTTCGGTCCCGGCTCGGCGCTT; GGCCTCTTCCCCGCCTACCAGCACTTAGCATCCCCCGCCCTGGTCTCCTGGAACTGGTGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014360 unedited; GGGGGCGCCATTGGGGGAATGGCAACTTNCCAGGNCCAGNAAAGCACTGGGGNAGGGTCA; CAGGGATGCCACCCGGGNATTCTGTTCAGGGAAACAGCTATGACCGCGGCCGCAATCTAG; AGTCGAGTCACCAGTTCCAGGAGACCAGGGCGGGGGATGCTAAGTGCTGGTAGGCGGGGA; AGAGGCCAAGCGCCGAGCCGGGACCGAAGGCAGGGTAGCCCGGCAGAGGGCAGGCGGCGG; CTGGAGGGGCGCCGCACTTCTCCTGGGCCGCGGCGGTTCCCACCTCGCCACCGCCGCCGC; CGCCGCACGGCTGCCCGTCGCGAACAAGCACCGGCACCACCACGCGACGCAGCAGGCCGG; GCGCGGCGTGCAGCTCGGCGGATGCTGCCAGGTCAGGCGACTCCGCCGCCCCTGGAGCGC; GAGCGCGCTTCAGCTTGTAGCGATGATTCTGGAACCAGATCTTGACCTGCGTGGGCGTGA; GGCGAAGCAGGCTCGCCAGCTGCTCGCGCTCGGGCGCAGACAGGTACCGCTGCTGCCGGA; AGCGCCGCTCCAACTCCAGCGTCTGCGCCTTGGAGAATAGCACCCGCCGCTTCTTCCTTT; TCTCGGCGTCCGAGCCCGGAGACGCGGGCCTAGCGGACGGCCGCTGCGACGAGTCTGGCG; GGCTGGTCTCCAGGCTGCTCTCGTCCGAGGAAGGGTAGTGGCCGCGCTCCGAATCCAGCC; AGGCGGCGCAAGGGTCGGGCTGGGGGGCGCGTGGTTCTGGCTCCCGCCTCGGCAGGTGTT; GCGCGTCCTGCTCGGGTAAATCTAGAAGGCTGCGCACGGTGAAGCTCAGGCGTCCAGAGG; TGGCCATGGCCNNAGAGGGGAAAGGAGCGGNGGCAGGGCAGGCTGGGGCACGNAGGGCNG; CCGGNACGCCCGCTCTN;
• Restriction Sites: Please inquire
• ACCN: NM_014360
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_014360.2, NP_055175.2
• RefSeq Size: 1857 bp
• RefSeq ORF: 720 bp
• Locus ID: 26257
• UniProt ID: O15522
• Cytogenetics: 14q13.3
• Protein Families: Druggable Genome, Transcription Factors
• Summary: The protein encoded by this gene is a homeobox-containing developmental regulator associated with liver development. The encoded protein binds to the alpha-fetoprotein (AFP) gene promoter and increases the expression of AFP. This gene is overexpressed in some lung cancers and is linked to poor patient survival, possibly due to its resistance to cisplatin. This gene is aberrantly methylated in pancreatic cancer, deleted in squamous cell lung carcinomas, and acts as a tumor suppressor in esophageal cancer. Mutations in this gene may also be a cause of neural tube defects. [provided by RefSeq, Dec 2015]