IL19 (NM_013371) Human Untagged Clone

SKU SC304003

IL19 (untagged)-Human interleukin 19 (IL19), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: IL19
• Synonyms: IL-10C; MDA1; NG.1; ZMDA1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_013371 edited; ATTTCCTCTTATCCACCTGAATAAAAATGACCAGCCCTTTCCAAATGGCAGAGAGCACTG; AGAGGAGACACAAGGAGCAGCCCGCAAGCACCAAGTGAGAGGCATGAAGTTACAGTGTGT; TTCCCTTTGGCTCCTGGGTACAATACTGATATTGTGCTCAGTAGACAACCACGGTCTCAG; GAGATGTCTGATTTCCACAGACATGCACCATATAGAAGAGAGTTTCCAAGAAATCAAAAG; AGCCATCCAAGCTAAGGACACCTTCCCAAATGTCACTATCCTGTCCACATTGGAGACTCT; GCAGATCATTAAGCCCTTAGATGTGTGCTGCGTGACCAAGAACCTCCTGGCGTTCTACGT; GGACAGGGTGTTCAAGGATCATCAGGAGCCAAACCCCAAAATCTTGAGAAAAATCAGCAG; CATTGCCAACTCTTTCCTCTACATGCAGAAAACTCTGCGGCAATGTCAGGAACAGAGGCA; GTGTCACTGCAGGCAGGAAGCCACCAATGCCACCAGAGTCATCCATGACAACTATGATCA; GCTGGAGGTCCACGCTGCTGCCATTAAATCCCTGGGAGAGCTCGACGTCTTTCTAGCCTG; GATTAATAAGAATCATGAAGTAATGTTCTCAGCTTGATGACAAGGAACCTGTATAGTGAT; CCAGGGATGAACACCCCCTGTGCGGTTTACTGTGGGAGACAGCCCAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013371 unedited; NAGGTCACATTTGTATACGACTCATATAGGGCGGCCGCGATTCAAATCTGGTACCGAGCT; CGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTAGCATTTCCTCTTATC; CACCTGAATAAAAATGACCAGCCCTTTCCAAATGGCAGAGAGCACTGAGAGGAGACACAA; GGAGCAGCCCGCAAGCACCAAGTGAGAGGCATGAAGTTACAGTGTGTTTCCCTTTGGCTC; CTGGGTACAATACTGATATTGTGCTCAGTAGACAACCACGGTCTCAGGAGATGTCTGATT; TCCACAGACATGCACCATATAGAAGAGAGTTTCCAAGAAATCAAAAGAGCCATCCAAGCT; AAGGACACCTTCCCAAATGTCACTATCCTGTCCACATTGGAGACTCTGCAGATCATTAAG; CCCTTAGATGTGTGCTGCGTGACCAAGAACCTCCTGGCGTTCTACGTGGACAGGGTGTTC; AAGGATCATCAGGAGCCAAACCCCAAAATCTTGAGAAAAATCAGCAGCATTGCCAACTCT; TTCCTCTACATGCAGAAAACTCTGCGGCAATGTCAGGAACAGAGGCAGTGTCACTGCAGG; CAGGAAGCCACCAATGCCACCAGAGTCATCCATGACAACTATGATCAGCTGGAGGTCCAC; GCTGCTGCCATTAAATCCCTGGGAGAGCTCGACGTCTTTCTAGCCTGGATTAATAAGAAT; CATGAAGTAATGTTCTCAGCTTGATGACAAGGAACCTGTATAGTGATCCAGGGATGAACA; CCCNCTGTGCGGTTTACTGTGGGAGACAGCCCACAGGGGCGAATTCTGCAGATATCCATC; ACACTGGGCGGCCGCTCGACTCTAGATTGCNGGCCGCGTCATAGCTGTTTCCCTGACAC;
• Restriction Sites: Please inquire
• ACCN: NM_013371
• Insert Size: 700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This construct is a cloned PCR fragment that was fully sequenced. There are no nucleotide differences between the OriGene clone and the NCBI reference ORF.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013371.2, NP_037503.2
• RefSeq Size: 1831 bp
• RefSeq ORF: 534 bp
• Locus ID: 29949
• UniProt ID: Q9UHD0
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway
• Summary: The protein encoded by this gene is a cytokine that belongs to the IL10 cytokine subfamily. This cytokine is found to be preferentially expressed in monocytes. It can bind the IL20 receptor complex and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). A similar cytokine in mouse is reported to up-regulate the expression of IL6 and TNF-alpha and induce apoptosis, which suggests a role of this cytokine in inflammatory responses. Alternatively spliced transcript variants encoding the distinct isoforms have been described. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) contains two alternate 5' non-coding exons, resulting in translation initiation from an in-frame downstream AUG start codon compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1. Variants 2 and 3 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.