DKK1 (NM_012242) Human Untagged Clone

CAT#: SC303946

DKK1 (untagged)-Human dickkopf homolog 1 (Xenopus laevis) (DKK1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DKK1
• Synonyms: DKK-1; SK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012242 edited
  CCCTGCAGTCAGGACTCTGGGACCGCAGGGGGCTCCCGGACCCTGACTCTGCAGCCGAAC
  CGGCACGGTTTCGTGGGGACCCAGGCTTGCAAAGTGACGGTCATTTTCTCTTTCTTTCTC
  CCTCTTGAGTCCTTCTGAGATGATGGCTCTGGGCGCAGCGGGAGCTACCCGGGTCTTTGT
  CGCGATGGTAGCGGCGGCTCTCGGCGGCCACCCTCTGCTGGGAGTGAGCGCCACCTTGAA
  CTCGGTTCTCAATTCCAACGCTATCAAGAACCTGCCCCCACCGCTGGGCGGCGCTGCGGG
  GCACCCAGGCTCTGCAGTCAGCGCCGCGCCGGGAATCCTGTACCCGGGCGGGAATAAGTA
  CCAGACCATTGACAACTACCAGCCGTACCCGTGCGCAGAGGACGAGGAGTGCGGCACTGA
  TGAGTACTGCGCTAGTCCCACCCGCGGAGGGGACGCAGGCGTGCAAATCTGTCTCGCCTG
  CAGGAAGCGCCGAAAACGCTGCATGCGTCACGCTATGTGCTGCCCCGGGAATTACTGCAA
  AAATGGAATATGTGTGTCTTCTGATCAAAATCATTTCCGAGGAGAAATTGAGGAAACCAT
  CACTGAAAGCTTTGGTAATGATCATAGCACCTTGGATGGGTATTCCAGAAGAACCACCTT
  GTCTTCAAAAATGTATCACACCAAAGGACAAGAAGGTTCTGTTTGTCTCCGGTCATCAGA
  CTGTGCCTCAGGATTGTGTTGTGCTAGACACTTCTGGTCCAAGATCTGTAAACCTGTCCT
  GAAAGAAGGTCAAGTGTGTACCAAGCATAGGAGAAAAGGCTCTCATGGACTAGAAATATT
  CCAGCGTTGTTACTGTGGAGAAGGTCTGTCTTGCCGGATACAGAAAGATCACCATCAAGC
  CAGTAATTCTTCTAGGCTTCACACTTGTCAGAGACACTAAACCAGCTATCCAAATGCAGT
  GAACTCCTTTTATATAATAGATGCTATGAAAACCTTTTATGACCTTCATCAACTCAATCC
  TAAGGATATACAAGTTCTGTGGTTTCAGTTAAGCATTCCAATAACACCTTCCAAAAAAAA
  AAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012242 unedited
  NAAGTCAACAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCCTGCA
  GTCAGGACTCTGGGACCGCAGGGGCTCCCGGACCCTGACTCTGCAGCCGAACCGGCACGG
  TTTCGTGGGGACCCAGGCTTGCAAAGTGACGGTCATTTTCTCTTTCTTTCTCCCTCTTGA
  GTCCTTCTGAGATGATGGCTCTGGGCGCAGCGGGAGCTACCCGGGTCTTTGTCGCGATGG
  TAGCGGCGGCTCTCGGCGGCCACCCTCTGCTGGGAGTGAGCGCCACCTTGAACTCGGTTC
  TCAATTCCAACGCTATCAAGAACCTGCCCCCACCGCTGGGCGGCGCTGCGGGGCACCCAG
  GCTCTGCAGTCAGCGCCGCGCCGGGAATCCTGTACCCGGGCGGGAATAAGTACCAGACCA
  TTGACAACTACCAGCCGTACCCGTGCGCAGAGGACGAGGAGTGCGGCACTGATGAGTACT
  GCGCTAGTCCCACCCGCGGAGGGGACGCATGCGTGCAAATCTGTCTCGCCTGCAGGAAGC
  GCCGAANACGCTGCATGCGTCACGCTATGTGCTGCCCCGGGAATTACTGCAAAAATGGAA
  TATGTGTGTCTTCTGATCAAAATCATTTCCGAGGAGAAATTGANGAAACCATCACTGANA
  GCTTTGGTAATGATCATAGCACCTTGGATGGGTATTCCAGAAGAACCACCTTGTCTTCAA
  AAATGTATCACACCANAGGACAAGAAGGTTCTGTTTGTCTCCGGTCATCAGACTGTGCCT
  CAAGATTGTGTTGTGCTAGACACTTCTGGGTCAAGATCTGNTAACCTGTCCTGAAAGAAG
  GTCAAGTGTGTACCAAGCATAGGAGGAAAGGCTCTCATGGACTAGAAATATTCCAGCGTT
  GNTACTGTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012242 unedited
  NACTTGTATATCCTTAGGATTGAGTTGATGAAGGTCATAAAAGGTTTTCATAGCATCTAT
  TATATAAAAGGAGTTCACTGCATTTGGATAGCTGGTTTAGTGTCTCTGACAAGTGTGAAG
  CCTAGAAGAATTACTGGCTTGATGGTGATCTTTCTGTATCCGGCAAGACAGACCTTCTCC
  ACAGTAACAACGCTGGAATATTTCTAGTCCATGAGAGCCTTTTCTCCTATGCTTGGTACA
  CACTTGACCTTCTTTCAGGACAGGTTTACAGATCTTGGACCAGAAGTGTCTAGCACAACA
  CAATCCTGAGGCACAGTCTGATGACCGGAGACAAACAGAACCTTCTTGTCCTTTGGTGTG
  ATACATTTTTGAAGACAAGGTGGTTCTTCTGGAATACCCATCCAAGGTGCTATGATCATT
  ACCAAAGCTTTCAGTGATGGTTTCCTCAATTTCTCCTCGGAAATGATTTTGATCAGAAGA
  CACACATATTCCATTTTTGCAGTAATTCCCGGGGCAGCACATAGCGTGACGCATGCAGCG
  TTTTCGGCGCTTCCTGCAGGCGAGACAGATTTGCACGCCTGCGTCCCCTCCGCGGGTGGG
  ACTAGCGCAGTACTCATCAGTGCCGCACTCCTCGTCCTCTGCGCACGGGTACGGCTGGTA
  GTTGTCAATGGTCTGGTACTTATTCCCGCCCGGGTACAGGATTCCCGGCGCGGCGCTGAC
  TGCAGAGCCTGGGTGCCCCGCAGCGCCGCCCCAGCGTGGGGGCAGGTTCTTGATAGCGTT
  GGAATTGAGAACCGAGTTCAAGGTGGCGCTCACTCCCAGCAGAGGGTGGCCGCCGAGAGC
  CGCCGCTACCATCGCGACAAAACCCGGGTAGCTCCCGCTGCGCCCAGAACATCA
  
• Restriction Sites: Please inquire
• ACCN: NM_012242
• Insert Size: 1103 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012242.2, NP_036374.1
• RefSeq Size: 1815 bp
• RefSeq ORF: 801 bp
• Locus ID: 22943
• UniProt ID: O94907
• Cytogenetics: 10q21.1
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Wnt signaling pathway
• Gene Summary: This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines. [provided by RefSeq, Sep 2017]