T (NM_003181) Human Untagged Clone

CAT#: SC303281

T (untagged)-Human T, brachyury homolog (mouse) (T)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: T
• Synonyms: SAVA; T; TFT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003181 edited
  TCTCAGGTAGCGAGTCTGGGCTTCGGGGACGGCGGGGAGGGGAGCCGGACGGGAGGATGA
  GCTCCCCTGGCACCGAGAGCGCGGGAAAGAGCCTGCAGTACCGAGTGGACCACCTGCTGA
  GCGCCGTGGAGAATGAGCTGCAGGCGGGCAGCGAGAAGGGCGACCCCACAGAGCGCGAAC
  TGCGCGTGGGCCTGGAGGAGAGCGAGCTGTGGCTGCGCTTCAAGGAGCTCACCAATGAGA
  TGATCGTGACCAAGAACGGCAGGAGGATGTTTCCGGTGCTGAAGGTGAACGTGTCTGGCC
  TGGACCCCAACGCCATGTACTCCTTCCTGCTGGACTTCGTGGCGGCGGACAACCACCGCT
  GGAAGTACGTGAACGGGGAATGGGTGCCGGGGGGCAAGCCGGAGCCGCAGGCGCCCAGCT
  GCGTCTACATCCACCCCGACTCGCCCAACTTCGGGGCCCACTGGATGAAGGCTCCCGTCT
  CCTTCAGCAAAGTCAAGCTCACCAACAAGCTCAACGGAGGGGGCCAGATCATGCTGAACT
  CCTTGCATAAGTATGAGCCTCGAATCCACATAGTGAGAGTTGGGGGTCCACAGCGCATGA
  TCACCAGCCACTGCTTCCCTGAGACCCAGTTCATAGCGGTGACTGCTTATCAGAACGAGG
  AGATCACAGCTCTTAAAATTAAGTACAATCCATTTGCAAAAGCTTTCCTTGATGCAAAGG
  AAAGAAGTGATCACAAAGAGATGATGGAGGAACCCGGAGACAGCCAGCAACCTGGGTACT
  CCCAATCAGGGGGGTGGCTTCTTCCTGGAACCAGCACCCTGTGTCCACCTGCAAATCCTC
  ATCCTCAGTTTGGAGGTGCCCTCTCCCTCCCCTCCACGCACAGCTGTGACAGGTACCCAA
  CCCTGAGGAGCCACCGGTCCTCACCCTACCCCAGCCCCTATGCTCATCGGAACAATTCTC
  CAACCTATTCTGACAACTCACCTGCATGTTTATCCATGCTGCAATCCCATGACAATTGGT
  CCAGCCTTGGAATGCCTGCCCATCCCAGCATGCTCCCCGTGAGCCACAATGCCAGCCCAC
  CTACCAGCTCCAGTCAGTACCCCAGCCTGTGGTCTGTGAGCAACGGCGCCGTCACCCCGG
  GCTCCCAGGCAGCAGCCGTGTCCAACGGGCTGGGGGCCCAGTTCTTCCGGGGCTCCCCCG
  CGCACTACACACCCCTCACCCATCCGGTCTCGGCGCCCTCTTCCTCGGGATCCCCACTGT
  ACGAAGGGGCGGCCGCGGCCACAGACATCGTGGACAGCCAGTACGACGCCGCAGCCCAAG
  GCCGCCTCATAGCCTCATGGACACCTGTGTCGCCACCTTCCATGTGAAGCAGCAAGGCCC
  AGGTCCCGAAAGATGCAGTGACTTTTTGTCGTGGCAGCCAGTGGTGACTGGATTGACCTA
  CTAGGTACCCAGTGGCAGTCTCAGGTTAAGAAGGAAATGCAGCCTCAGTAACTTCCTTTT
  CAAAGCAGTGGAGGAGCACAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003181 unedited
  GNNCGGCCGCGNAATTCGCCCTTGNAGCAGGNAGGTGGATTCTCAGTAGCGAGTCTGGGC
  TTCGGGGACGGCGGGGAGGGGAGCCGGACGGGAGGATGAGCTCCCCTGGCACCGAGAGCG
  CGGGAAAGAGCCTGCAGTACCGAGTGGACCACCTGCTGAGCGCCGTGGAGAATGAGCTGC
  AGGCGGGCAGCGAGAAGGGCGACCCCACAGAGCGCGAACTGCGCGTGGGCCTGGAGGAGA
  GCGAGCTGTGGCTGCGCTTCAAGGAGCTCACCAATGAGATGATCGTGACCAAGAACGGCA
  GGAGGATGTTTCCGGTGCTGAAGGTGAACGTGTCTGGCCTGGACCCCAACGCCATGTACT
  CCTTCCTGCTGGACTTCGTGGCGGCGGACAACCACCGCTGGAAGTACGTGAACGGNGAAT
  GGGTGCCGGGGGGCAAGCCGGAGCCGCAGGCGCCCAGCTGCGTCTACATCCACCCCGACT
  CGCCCAACTTCGGGGCCCACTGGATGAAGGCTCCCGTCTCCTTCAGCANAGTCAAGCTCA
  CCAACNAGCTCAACGGGAGGNGGCCAGATCATGCTGAACTCCTTTGCATAGTATGAGCCT
  CGAATCCACATAGTGAGAGTTGGGGGGTCCACAGCGCATGATCACCAGCCACTGCTTCCC
  TGAGACCCAGTTCATAGCGGTGACTGCTTATCAGAACGAGGAGATCACAGCTCTTAAAAT
  TAAGTANCATCCATTTGCAAAAGCTTTCCTTGATGCANAGGANAGAAGTGATCACAAAGA
  GATGAATGGANGACCCGNAGACAGCCAGCAACCTGGGTACTCCCATCA
  
  3' Read Nucleotide Sequence

  >Reverse primer walk for NM_003181 unedited
  GACTTCTGGATAGTCAATCCATTCCCACTGGCTGCCACGAAAAAATCACTGCATCTTTCG
  GGACCTGGGCCTTGCTGCTTCACATGGAAGGTGGCGACACAGGTGTCCATGAGGCTATGA
  GGCGGCCTTGGGCTGCGGCGTCGTACTGGCTGTCCACGATGTCTGTGGCCGCGGCCGCCC
  CTTCGTACAGTGGGGATCCCGAGGAAGAGGGCGCCGAGACCGGATGGGTGAGGGGTGTGT
  AGTGCGCGGGGGAGCCCCGGAAGAACTGGGCCCCCAGCCCGTTGGACACGGCTGCTGCCT
  GGGAGCCCGGGGTGACGGCGCCGTTGCTCACAGACCACAGGCTGGGGTACTGACTGGAGC
  TGGTAGGTGGGCTGGCATTGTGGCTCACGGGGAGCATGCTGGGATGGGCAGGCATTCCAA
  GGCTGGACCAATTGTCATGGGATTGCAGCATGGATAAACATGCAGGTGAGTTGTCAGAAT
  AGGTTGGAGAATTGTTCCGATGAGCATAGGGGCTGGGGTAGGGTGAGGACCGGTGGCTCC
  TCAGGGTTGGGTACCTGTCACAGCTGTGCGTGGAGGGGAGGGAGAGGGCACCTCCAAACT
  GAGGATGAGGATTTGCAGGTGGACACAGGGTGCTGGTTCCAGGAAGAAGCCACCCCCCTG
  ATTGGGAGTACCCAGGTTGCTGGCTGTCTCCGGGTTCCTCCATCATCTCTTTGTGATCAC
  TTCTTTCCTTTGCATCAAGGAAAGCTTTTGCAAATGGATTGTACTTAATTTTAAGAGCTG
  TGATCTCCTCGTTCTGATAAGCAGTCACCGCTATGAACTGGGTCTCAGGGAAGCAGTGGC
  TA
  
• Restriction Sites: Please inquire
• ACCN: NM_003181
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There is 4 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the insertion of 1aa and substituion of 1 aa.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003181.2, NP_003172.1
• RefSeq Size: 2518 bp
• RefSeq ORF: 1308 bp
• Locus ID: 6862
• UniProt ID: O15178
• Cytogenetics: 6q27
• Protein Families: ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies. [provided by RefSeq, Sep 2018]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.