ERK1 (MAPK3) (NM_002746) Human Untagged Clone

CAT#: SC303240

MAPK3 (untagged)-Human mitogen-activated protein kinase 3 (MAPK3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ERK1
• Synonyms: ERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002746 edited
  CGGCTCAGATGGCGGCGGCGGCGGCTCAGGGGGGCGGGGGCGGGGAGCCCCGTAGAACCG
  AGGGGGTCGGCCCGGGGGTCCCGGGGGAGGTGGAGATGGTGAAGGGGCAGCCGTTCGACG
  TGGGCCCGCGCTACACGCAGTTGCAGTACATCGGCGAGGGCGCGTACGGCATGGTCAGCT
  CGGCCTATGACCACGTGCGCAAGACTCGCGTGGCCATCAAGAAGATCAGCCCCTTCGAAC
  ATCAGACCTACTGCCAGCGCACGCTCCGGGAGATCCAGATCCTGCTGCGCTTCCGCCATG
  AGAATGTCATCGGCATCCGAGACATTCTGCGGGCGTCCACCCTGGAAGCCATGAGAGATG
  TCTACATTGTGCAGGACCTGATGGAGACTGACCTGTACAAGTTGCTGAAAAGCCAGCAGC
  TGAGCAATGACCATATCTGCTACTTCCTCTACCAGATCCTGCGGGGCCTCAAGTACATCC
  ACTCCGCCAACGTGCTCCACCGAGATCTAAAGCCCTCCAACCTGCTCATCAACACCACCT
  GCGACCTTAAGATTTGTGATTTCGGCCTGGCCCGGATTGCCGATCCTGAGCATGACCACA
  CCGGCTTCCTGACGGAGTATGTGGCTACGCGCTGGTACCGGGCCCCAGAGATCATGCTGA
  ACTCCAAGGGCTATACCAAGTCCATCGACATCTGGTCTGTGGGCTGCATTCTGGCTGAGA
  TGCTCTCTAACCGGCCCATCTTCCCTGGCAAGCACTACCTGGATCAGCTCAACCACATTC
  TGGGCATCCTGGGCTCCCCATCCCAGGAGGACCTGAATTGTATCATCAACATGAAGGCCC
  GAAACTACCTACAGTCTCTGCCCTCCAAGACCAAGGTGGCTTGGGCCAAGCTTTTCCCCA
  AGTCAGACTCCAAAGCCCTTGACCTGCTGGACCGGATGTTAACCTTTAACCCCAATAAAC
  GGATCACAGTGGAGGAAGCGCTGGCTCACCCCTACCTGGAGCAGTACTATGACCCGACGG
  ATGAGCCAGTGGCCGAGGAGCCCTTCACCTTCGCCATGGAGCTGGATGACCTACCTAAGG
  AGCGGCTGAAGGAGCTCATCTTCCAGGAGACAGCACGCTTCCAGCCCGGAGTGCTGGAGG
  CCCCCTAGCCCAGACAGACATCTCTGCACCCTGGGGCC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002746 unedited
  GATTTGTATACGACTGACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCTCAGATGGC
  GGCGGCGGCGGCTCAGGGGGGCGGGGGCGGGGAGCCCCGTAGAACCGAGGGGGTCGGCCC
  GGGGGTCCCGGGGGAGGTGGAGATGGTGAAGGGGCAGCCGTTCGACGTGGGCCCGCGCTA
  CACGCAGTTGCAGTACATCGGCGAGGGCGCGTACGGCATGGTCAGCTCGGCCTATGACCA
  CGTGCGCAAGACTCGCGTGGCCATCAAGAAGATCAGCCCCTTCGAACATCAGACCTACTG
  CCAGCGCACGCTCCGGGAGATCCAGATCCTGCTGCGCTTCCGCCATGAGAATGTCATCGG
  CATCCGAGACATTCTGCGGGCGTCCACCCTGGAAGCCATGAGAGATGTCTACATTGTGCA
  GGACCTGATGGAGACTGACCTGTACAAGTTGCTGAAAAGCCAGCAGCTGAGCAATGACCA
  TATCTGCTACTTCCTCTACCAGATCCTGCGGGGCCTCAAGTACATCCACTCCGCCAACGT
  GCTCCACCGAGATCTAAAGCCCTCCCACCTGCTCATCAACACCACCTGCGACCTTAAGAA
  TTGTGATTTCCGCCTGCCCGGTATGCCGATCCTGAGCATGACCACACCGGCTTCCTGACG
  GAGTATGTGGCTACGCGCCTGTACCGGGCCCCAGAAATATGCCGAACTCCAGGGCTATAC
  CAAGTCATCGACTCTGTCCGTGGCTCGCTTCTGGTGANAGCTTTTACCGCCCATCTTCTG
  GAGGCCTTACTGTTACTAACCCATTTGGCATCCTGGGTCCATCCGGAGGCTGGATGGTCT
  AATTGAGGCCGAAT
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_002746 unedited
  GCTCATCTTCCAGAGACAGCACGCTTCCAGCCCGGAGTGNTAGAGGCCCCCTAGCCCAGA
  CAGACATCTCTGCACCCTGGGGCCTGGACCTGCCTCCTGCCTGCCCCTCTCCCGCCAGAC
  TGTTAGAAAATGGACACTGTGCCCAGCCCGGACCTTGGCAGCCCAGGCCGGGGTGGAGCA
  TGGGCCTGGCCACCTCTCTCCTTTGCTGAGGCCTCCAGCTTCAGGCAGGCCAAGGCCTTC
  TCCTCCCCACCCGCCCTCCCCACGGGGCCTCGGGACCTCAGGTGGCCCCAGTTCAATCTC
  CCGCTGCTGCTGCTGCGCCCTTACCTTCCCCAGCGTCCCAGTCTCTGGCAGTTCTGGAAT
  GGAAGGGTTCTGGCTGCCCCAACCTGCTGAAGGGCAAAGGTGGAGGGTGGGGGGCGCTGA
  GTAGGGACTCAGGGCCATGCCTGCCCCCCTCATCTCATTCAAACCCCACCCTAGTTTCCC
  TGAAGGAACATTCCTTAGTCTCAAGGGCTAGCATCCCTGAGGAGCCAGGCCGGGCCGAAT
  CCCCTCCCTGTCCAAACTGTGACCTTTCGTGGCCTTGGTGTGCTTCTGTGTGTGGTGAGC
  CAAAATTGGACCTGGGGGCGTGNAAAGCCCGGCGCCCCTGCAACCCTCCTGACCCGTCTA
  ATATATAATTATAGAGATGTGTCTATGAAAAAAAAAAAAAAAAACTTCGACTCTAGATTG
  CGGCCGCGGGCATACTTGTTCCTGAACAGTATCGGC
  
• Restriction Sites: Please inquire
• ACCN: NM_002746
• Insert Size: 2200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers this to be a polymorphism and to reflect the natural differences between individuals. These result in the substitution of 1 amino acid.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002746.1, NP_002737.1
• RefSeq Size: 1866 bp
• RefSeq ORF: 1140 bp
• Locus ID: 5595
• UniProt ID: P27361
• Cytogenetics: 16p11.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, Adherens junction, Alzheimer's disease, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Non-small cell lung cancer, Oocyte meiosis, Pancreatic cancer, Pathways in cancer, Prion diseases, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, TGF-beta signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Type II diabetes mellitus, Vascular smooth muscle contraction, VEGF signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the most common transcript and encodes isoform 1.