IL7R alpha (IL7R) (NM_002185) Human Untagged Clone

CAT#: SC303141

IL7R (untagged)-Human interleukin 7 receptor (IL7R)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IL7R alpha
• Synonyms: CD127; CDW127; IL-7R-alpha; IL7RA; ILRA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002185 edited
  CCTCCCTCCCTTCCTCTTACTCTCATTCATTTCATACACACTGGCTCACACATCTACTCT
  CTCTCTCTATCTCTCTCAGAATGACAATTCTAGGTACAACTTTTGGCATGGTTTTTTCTT
  TACTTCAAGTCGTTTCTGGAGAAAGTGGCTATGCTCAAAATGGAGACTTGGAAGATGCAG
  AACTGGATGACTACTCATTCTCATGCTATAGCCAGTTGGAAGTGAATGGATCGCAGCACT
  CACTGACCTGTGCTTTTGAGGACCCAGATGTCAACATCACCAATCTGGAATTTGAAATAT
  GTGGGGCCCTCGTGGAGGTAAAGTGCCTGAATTTCAGGAAACTACAAGAGATATATTTCA
  TCGAGACAAAGAAATTCTTACTGATTGGAAAGAGCAATATATGTGTGAAGGTTGGAGAAA
  AGAGTCTAACCTGCAAAAAAATAGACCTAACCACTATAGTTAAACCTGAGGCTCCTTTTG
  ACCTGAGTGTCGTCTATCGGGAAGGAGCCAATGACTTTGTGGTGACATTTAATACATCAC
  ACTTGCAAAAGAAGTATGTAAAAGTTTTAATGCACGATGTAGCTTACCGCCAGGAAAAGG
  ATGAAAACAAATGGACGCATGTGAATTTATCCAGCACAAAGCTGACACTCCTGCAGAGAA
  AGCTCCAACCGGCAGCAATGTATGAGATTAAAGTTCGATCCATCCCTGATCACTATTTTA
  AAGGCTTCTGGAGTGAATGGAGTCCAAGTTATTACTTCAGAACTCCAGAGATCAATAATA
  GCTCAGGGGAGATGGATCCTATCTTACTAACCATCAGCATTTTGAGTTTTTTCTCTGTCG
  CTCTGTTGGTCATCTTGGCCTGTGTGTTATGGAAAAAAAGGATTAAGCCTATCGTATGGC
  CCAGTCTCCCCGATCATAAGAAGACTCTGGAACATCTTTGTAAGAAACCAAGAAAAAATT
  TAAATGTGAGTTTCAATCCTGAAAGTTTCCTGGACTGCCAGATTCATAGGGTGGATGACA
  TTCAAGCTAGAGATGAAGTGGAAGGTTTTCTGCAAGATACGTTTCCTCAGCAACTAGAAG
  AATCTGAGAAGCAGAGGCTTGGAGGGGATGTGCAGAGCCCCAACTGCCCATCTGAGGATG
  TAGTCATCACTCCAGAAAGCTTTGGAAGAGATTCATCCCTCACATGCCTGGCTGGGAATG
  TCAGTGCATGTGACGCCCCTATTCTCTCCTCTTCCAGGTCCCTAGACTGCAGGGAGAGTG
  GCAAGAATGGGCCTCATGTGTACCAGGACCTCCTGCTTAGCCTTGGGACTACAAACAGCA
  CGCTGCCCCCTCCATTTTCTCTCCAATCTGGAATCCTGACATTGAACCCAGTTGCTCAGG
  GTCAGCCCATTCTTACTTCCCTGGGATCAAATCAAGAAGAAGCATATGTCACCATGTCCA
  GCTTCTACCAAAACCAGTGAAGTGTAAGAAACCCAGACTGAACTTACCGTGAGCGACAAA
  GATGATTTAAAAGGGAAGTCTAGAGTTCCTAGTCTCCCTCACAGCACAGAGAAGACAAAA
  TTAGCAAAACCCCACTACACAGTCTGCAAGATTCTGAAACATTGCTTTGACCACTCTTCC
  TGAGTTCAGTGGCACTCAACATGAGTCAAGAGCATCCTGCTTCTACCATGTGGATTTGGT
  CAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002185 unedited
  CGTCATTATTGTATACGACTCATATAGGCGGCCGCGNATTCGCCCTTCCTCCCTCCCTTC
  CTCTTACTCTCATTCATTTCATACACACTGGCTCACACATCTACTCTCTCTCTCTATCTC
  TCTCAGAATGACAATTCTAGGTACAACTTTTGGCATGGTTTTTTCTTTACTTCAAGTCGT
  TTCTGGAGAAAGTGGCTATGCTCAAAATGGAGACTTGGAAGATGCAGAACTGGATGACTA
  CTCATTCTCATGCTATAGCCAGTTGGAAGTGAATGGATCGCAGCACTCACTGACCTGTGC
  TTTTGAGGACCCAGATGTCAACATCACCAATCTGGAATTTGAAATATGTGGGGCCCTCGT
  GGAGGTAAAGTGCCTGAATTTCAGGAAACTACAAGAGATATATTTCATCGAGACAAAGAA
  ATTCTTACTGATTGGAAAGAGCAATATATGTGTGAAGGTTGGAGAAAAGAGTCTAACCTG
  CAAAAAAATAGACCTAACCACTATAGTTAAACCTGAGGCTCCTTTTGACCTGAGTGTCGT
  CTATCGGGAAGGAGCCAATGACTTTGTGGTGACATTTAATACATCACACTTGCAAAAGAA
  GTATGTAAAAGTTTTAATGCACGATGTAGCTTACCGCCAGGAAAAGGATGAAAACAAATG
  GACGCATGTGAATTTATCCAGCACAAAGCTGACACTCCTGCAGAGAAAGCTCCAACNNCG
  CAGCATGTATGAGATTAAAAGTCGATCCATCCCTGATCACTATTTTTAAAGGCTTCTGGA
  GTGAATGGAGTCCAAGTTATTACTTCAGAACTCCAGAGATCAATATC
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_002185 unedited
  CACTATCTNAGGTGGNTGACATTCAAGCTAGNANATGAAGTGGAAGTTTTCTGCAAGAAC
  GTTTCCTCAGCAACTAGAAGAATCTGAGAAGCAGAGGCTTGGAGGGGATGTGCAGAGCCC
  CAACTGCCCATCTGAGGATGTAGTCATCACTCCAGAAAGCTTTGGAAGAGATTCATCCCT
  CACATGCCTGGCTGGGAATGTCAGTGCATGTGACGCCCCTATTCTCTCCTCTTCCAGGTC
  CCTAGACTGCAGGGAGAGTGGCAAGAATGGGCCTCATGTGTACCAGGACCTCCTGCTTAG
  CCTTGGGACTACAAACAGCACGCTGCCCCCTCCATTTTCTCTCCAATCTGGAATCCTGAC
  ATTGAACCCAGTTGCTCAGGGTCAGCCCATTCTTACTTCCCTGGGATCAAATCAAGAAGA
  AGCATATGTCACCATGTCCAGCTTCTACCAAAACCAGTGAAGTGTAAGAAACCCAGACTG
  AACTTACCGTGAGCGACAAAGATGATTTAAAAGGGAAGTCTAGAGTTCCTAGTCTCCCTC
  ACAGCACAGAGAAGACAAAATTAGCAAAACCCCACTACACAGTCTGCAAGATTCTGAAAC
  ATTGCTTTGACCACTCTTCCTGAGTTCAGTGGCACTCAACATGAGTCAAGAGCATCCTGC
  TTCTACCATGTGGATTTGGTCAAAGGGCGAATTCAGATCTGGTACCGATATCAAGCTTGT
  CGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCN
  TGTGACCCCTNCCCAGTGCCTCTCCTGGCCCTGGNAAGTGCCACTCCAGTGCN
  
• Restriction Sites: Please inquire
• ACCN: NM_002185
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002185.2, NP_002176.2
• RefSeq Size: 1809 bp
• RefSeq ORF: 1380 bp
• Locus ID: 3575
• UniProt ID: P16871
• Cytogenetics: 5p13.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway, Primary immunodeficiency
• Gene Summary: The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (1) encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.