SH3KBP1 (NM_001024666) Human Untagged Clone

SKU SC302281

SH3KBP1 (untagged)-Human SH3-domain kinase binding protein 1 (SH3KBP1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: SH3KBP1
• Synonyms: AGMX2; CD2BP3; CIN85; GIG10; HSB-1; HSB1; IMD61; MIG18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC302281 representing NM_001024666.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGAGGTTTCAGCAGCCAAAGCCCCAAGTGCCGCAGACTTGTCTGAGATTGAAATAAAGAAAGAGATG; AAGAAAGACCCTCTCACCAACAAAGCTCCAGAAAAGCCCCTGCACGAAGTGCCCAGTGGAAACTCTTTG; CTGTCTTCTGAAACGATTTTAAGAACCAATAAGAGAGGCGAGCGACGGAGGCGCCGGTGCCAGGTGGCA; TTCAGCTACCTGCCCCAGAATGACGATGAACTTGAGCTGAAAGTTGGCGACATCATAGAGGTGGTAGGA; GAGGTAGAGGAAGGATGGTGGGAAGGTGTTCTCAACGGGAAGACTGGAATGTTTCCTTCCAACTTCATC; AAGGAGCTGTCAGGGGAGTCGGATGAGCTTGGCATTTCCCAGGATGAGCAGCTATCCAAGTCAAGTTTA; AGGGAAACCACAGGCTCCGAGAGTGATGGGGGTGACTCAAGCAGCACCAAGTCTGAAGGTGCCAACGGG; ACAGTGGCAACTGCAGCAATCCAGCCCAAGAAAGTTAAGGGAGTGGGCTTTGGAGACATTTTCAAAGAC; AAGCCAATCAAACTAAGACCAAGGTCAATTGAAGTAGAAAATGACTTTCTGCCGGTAGAAAAGACTATT; GGGAAGAAGTTACCTGCAACTACAGCAACTCCAGACTCATCAAAAACAGAAATGGACAGCAGGACAAAG; AGCAAGGATTACTGCAAAGTAATATTTCCATATGAGGCACAGAATGATGATGAATTGACAATCAAAGAA; GGAGATATAGTCACTCTCATCAATAAGGACTGCATCGACGTAGGCTGGTGGGAAGGAGAGCTGAACGGC; AGACGAGGCGTGTTCCCCGATAACTTCGTGAAGTTACTTCCACCGGACTTTGAAAAGGAAGGGAATAGA; CCCAAGAAGCCACCGCCTCCATCCGCTCCTGTCATCAAACAAGGGGCAGGCACCACTGAGAGAAAACAT; GAAATTAAAAAGATACCTCCTGAAAGACCAGAAATGCTTCCAAACAGAACAGAAGAAAAAGAAAGACCA; GAGAGAGAGCCAAAACTGGATTTACAGAAGCCCTCCGTTCCTGCCATACCGCCAAAAAAGCCTCGGCCA; CCTAAGACCAATTCTCTCAGCAGACCTGGCGCACTGCCCCCGAGAAGGCCGGAGAGACCGGTGGGTCCG; CTGACACACACCAGGGGTGACAGTCCAAAGATTGACTTGGCCGGCAGTTCGCTATCTGGCATCCTGGAC; AAAGATCTCTCGGACCGCAGCAATGACATTGACTTAGAAGGTTTTGACTCCGTGGTATCATCTACTGAG; AAACTCAGTCATCCGACCACAAGCAGACCAAAAGCTACAGGGAGGCGGCCTCCGTCCCAGTCCCTCACA; TCTTCATCCCTTTCAAGCCCTGATATCTTCGACTCCCCAAGTCCCGAAGAGGATAAGGAGGAACACATT; TCACTTGCGCACAGAGGAGTGGACGCGTCAAAGAAAACTTCCAAGACTGTTACCATATCCCAAGTGTCT; GACAACAAAGCATCCCTGCCGCCCAAGCCGGGGACCATGGCAGCAGGTGGCGGTGGGCCAGCCCCTCTG; TCCTCAGCGGCGCCCTCCCCCCTGTCATCCTCTTTGGGAACAGCTGGACACAGAGCCAACTCCCCGTCT; CTGTTCGGCACGGAAGGAAAACCAAAGATGGAGCCTGCGGCCAGCAGCCAGGCGGCCGTGGAGGAGCTA; AGGACACAGGTCCGCGAGCTGAGGAGCATCATCGAGACCATGAAGGACCAGCAGAAACGAGAGATTAAA; CAGTTATTGTCTGAGTTGGATGAAGAGAAGAAAATCCGGCTTCGGTTGCAGATGGAAGTGAACGACATA; AAGAAAGCTCTACAATCAAAATGA; ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAG; GATGACGACGATAAGGTTTAAACGGCCGGCCGCGGT;
• Restriction Sites: SgfI-NotI
• ACCN: NM_001024666
• Insert Size: 1887 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001024666.2
• RefSeq Size: 4454 bp
• RefSeq ORF: 1887 bp
• Locus ID: 30011
• UniProt ID: Q96B97
• Cytogenetics: Xp22.12
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis
• MW: 68.6 kDa
• Summary: This gene encodes an adapter protein that contains one or more N-terminal Src homology domains, a proline rich region and a C-terminal coiled-coil domain. The encoded protein facilitates protein-protein interactions and has been implicated in numerous cellular processes including apoptosis, cytoskeletal rearrangement, cell adhesion and in the regulation of clathrin-dependent endocytosis. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2017]; Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is shorter than isoform 1.