TLR10 (NM_001017388) Human Untagged Clone

SKU SC302028

TLR10 (untagged)-Human toll-like receptor 10 (TLR10), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: TLR10
• Synonyms: CD290
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC302028 representing NM_001017388.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGAGACTCATCAGAAACATTTACATATTTTGTAGTATTGTTATGACAGCAGAGGGTGATGCTCCAGAG; CTGCCAGAAGAAAGGGAACTGATGACCAACTGCTCCAACATGTCTCTAAGAAAGGTTCCCGCAGACTTG; ACCCCAGCCACAACGACACTGGATTTATCCTATAACCTCCTTTTTCAACTCCAGAGTTCAGATTTTCAT; TCTGTCTCCAAACTGAGAGTTTTGATTCTATGCCATAACAGAATTCAACAGCTGGATCTCAAAACCTTT; GAATTCAACAAGGAGTTAAGATATTTAGATTTGTCTAATAACAGACTGAAGAGTGTAACTTGGTATTTA; CTGGCAGGTCTCAGGTATTTAGATCTTTCTTTTAATGACTTTGACACCATGCCTATCTGTGAGGAAGCT; GGCAACATGTCACACCTGGAAATCCTAGGTTTGAGTGGGGCAAAAATACAAAAATCAGATTTCCAGAAA; ATTGCTCATCTGCATCTAAATACTGTCTTCTTAGGATTCAGAACTCTTCCTCATTATGAAGAAGGTAGC; CTGCCCATCTTAAACACAACAAAACTGCACATTGTTTTACCAATGGACACAAATTTCTGGGTTCTTTTG; CGTGATGGAATCAAGACTTCAAAAATATTAGAAATGACAAATATAGATGGCAAAAGCCAATTTGTAAGT; TATGAAATGCAACGAAATCTTAGTTTAGAAAATGCTAAGACATCGGTTCTATTGCTTAATAAAGTTGAT; TTACTCTGGGACGACCTTTTCCTTATCTTACAATTTGTTTGGCATACATCAGTGGAACACTTTCAGATC; CGAAATGTGACTTTTGGTGGTAAGGCTTATCTTGACCACAATTCATTTGACTACTCAAATACTGTAATG; AGAACTATAAAATTGGAGCATGTACATTTCAGAGTGTTTTACATTCAACAGGATAAAATCTATTTGCTT; TTGACCAAAATGGACATAGAAAACCTGACAATATCAAATGCACAAATGCCACACATGCTTTTCCCGAAT; TATCCTACGAAATTCCAATATTTAAATTTTGCCAATAATATCTTAACAGACGAGTTGTTTAAAAGAACT; ATCCAACTGCCTCACTTGAAAACTCTCATTTTGAATGGCAATAAACTGGAGACACTTTCTTTAGTAAGT; TGCTTTGCTAACAACACACCCTTGGAACACTTGGATCTGAGTCAAAATCTATTACAACATAAAAATGAT; GAAAATTGCTCATGGCCAGAAACTGTGGTCAATATGAATCTGTCATACAATAAATTGTCTGATTCTGTC; TTCAGGTGCTTGCCCAAAAGTATTCAAATACTTGACCTAAATAATAACCAAATCCAAACTGTACCTAAA; GAGACTATTCATCTGATGGCCTTACGAGAACTAAATATTGCATTTAATTTTCTAACTGATCTCCCTGGA; TGCAGTCATTTCAGTAGACTTTCAGTTCTGAACATTGAAATGAACTTCATTCTCAGCCCATCTCTGGAT; TTTGTTCAGAGCTGCCAGGAAGTTAAAACTCTAAATGCGGGAAGAAATCCATTCCGGTGTACCTGTGAA; TTAAAAAATTTCATTCAGCTTGAAACATATTCAGAGGTCATGATGGTTGGATGGTCAGATTCATACACC; TGTGAATACCCTTTAAACCTAAGGGGAACTAGGTTAAAAGACGTTCATCTCCACGAATTATCTTGCAAC; ACAGCTCTGTTGATTGTCACCATTGTGGTTATTATGCTAGTTCTGGGGTTGGCTGTGGCCTTCTGCTGT; CTCCACTTTGATCTGCCCTGGTATCTCAGGATGCTAGGTCAATGCACACAAACATGGCACAGGGTTAGG; AAAACAACCCAAGAACAACTCAAGAGAAATGTCCGATTCCACGCATTTATTTCATACAGTGAACATGAT; TCTCTGTGGGTGAAGAATGAATTGATCCCCAATCTAGAGAAGGAAGATGGTTCTATCTTGATTTGCCTT; TATGAAAGCTACTTTGACCCTGGCAAAAGCATTAGTGAAAATATTGTAAGCTTCATTGAGAAAAGCTAT; AAGTCCATCTTTGTTTTGTCTCCCAACTTTGTCCAGAATGAGTGGTGCCATTATGAATTCTACTTTGCC; CACCACAATCTCTTCCATGAAAATTCTGATCATATAATTCTTATCTTACTGGAACCCATTCCATTCTAT; TGCATTCCCACCAGGTATCATAAACTGAAAGCTCTCCTGGAAAAAAAAGCATACTTGGAATGGCCCAAG; GATAGGCGTAAATGTGGGCTTTTCTGGGCAAACCTTCGAGCTGCTATTAATGTTAATGTATTAGCCACC; AGAGAAATGTATGAACTGCAGACATTCACAGAGTTAAATGAAGAGTCTCGAGGTTCTACAATCTCTCTG; ATGAGAACAGATTGTCTATAA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001017388
• Insert Size: 2436 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001017388.2
• RefSeq Size: 3484 bp
• RefSeq ORF: 2436 bp
• Locus ID: 81793
• UniProt ID: Q9BXR5
• Cytogenetics: 4p14
• Protein Families: Druggable Genome, Transmembrane
• MW: 94.6 kDa
• Summary: The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is most highly expressed in lymphoid tissues such as spleen, lymph node, thymus, and tonsil. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Aug 2010]; Transcript Variant: This variant (2) lacks two exons in the 5' UTR, compared to variant 1. Variants 1-4 encode the same protein (isoform a).