GGA1 (NM_001001560) Human Untagged Clone

SKU SC300217

GGA1 (untagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: GGA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC300217 representing NM_001001560.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGAGCCCGCGATGGAGCCGGAGACTCTGGAGGCGCGAATCAATAGAGCCACGAACCCCCTGAACAAG; GAGCTCGACTGGGCCAGCATCAACGGCTTCTGCGAGCAGCTCAACGAGGACTTTGAGGGGCCTCCACTC; GCCACCCGGCTGCTGGCCCACAAGATCCAGTCCCCACAGGAGTGGGAGGCGATCCAGGCCTTGACGGTG; CTGGAAACATGCATGAAGAGCTGCGGCAAGCGGTTCCACGACGAAGTGGGCAAGTTCCGCTTTCTCAAC; GAGCTCATCAAGGTCGTGTCTCCCAAGTATCTGGGCTCTCGGACATCGGAGAAGGTGAAGAACAAGATC; TTGGAGCTCCTCTACAGCTGGACAGTGGGCCTGCCCGAGGAGGTGAAAATCGCAGAGGCCTACCAGATG; CTAAAGAAGCAGGGGATTGTAAAGTCCGACCCCAAGCTTCCAGATGACACTACCTTTCCCCTTCCTCCT; CCACGGCCGAAGAATGTGATCTTTGAAGATGAGGAGAAATCCAAGATGCTGGCCCGCCTGCTGAAGAGC; TCCCATCCCGAAGACCTCCGCGCAGCCAATAAGCTCATCAAAGAGATGGTGCAGGAGGACCAGAAGCGG; ATGGAGAAGATCTCGAAGAGGGTGAATGCCATCGAGGAGGTGAACAACAATGTGAAACTGCTCACGGAG; ATGGTGATGAGCCACAGCCAGGGCGGCGCAGCAGCTGGCAGCAGCGAGGACCTCATGAAGGAACTGTAC; CAGCGCTGTGAGCGGATGCGGCCCACGCTCTTCCGACTGGCGAGTGACACAGAGGACAATGATGAGGCC; TTAGGCCTCAGTGACCCCACACCCCCTTCAGGCCCAAGCCTGGATGGTACCGGATGGAACAGCTTCCAG; TCGTCGGATGCCACTGAGCCCCCAGCCCCTGCTCTGGCCCAGGCCCCCAGTATGGAAAGCCGACCCCCA; GCGCAGACATCCCTGCCAGCAAGCAGCGGTCTGGACGACCTAGACCTCCTGGGGAAGACCCTCCTGCAG; CAGTCGCTGCCCCCGGAATCCCAGCAAGTGCGGTGGGAGAAGCAGCAGCCAACCCCCCGGCTCACACTC; CGGGACCTGCAGAATAAGAGCAGCAGCTGCAGCTCCCCCAGCTCCAGCGCCACCAGCCTTCTCCACACC; GTGTCCCCAGAGCCCCCCAGGCCTCCGCAGCAGCCCGTACCAACCGAGCTCTCACTGGCCAGCATCACT; GTGCCCCTGGAGTCCATCAAACCCAGCAACATCCTGCCCGTGACTGTGTATGACCAGCACGGCTTCCGC; ATCCTCTTCCATTTTGCCCGGGACCCACTGCCAGGGCGCTCCGACGTGCTGGTGGTGGTGGTTTCCATG; CTGAGCACCGCCCCCCAGCCCATCCGCAACATCGTGTTCCAGTCAGCTGTCCCCAAGGTTATGAAGGTG; AAGCTGCAGCCACCCTCGGGCACGGAGCTGCCAGCTTTTAACCCCATCGTCCACCCCTCAGCAATCACC; CAGGTCCTGCTGCTTGCCAACCCCCAGAAGGAGAAGGTTCGCCTCCGCTACAAGCTCACCTTCACCATG; GGTGACCAGACCTACAACGAGATGGGGGATGTGGACCAGTTCCCCCCACCTGAAACCTGGGGTAGCCTC; TAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001001560
• Insert Size: 1659 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001001560.2
• RefSeq Size: 2924 bp
• RefSeq ORF: 1659 bp
• Locus ID: 26088
• UniProt ID: Q9UJY5
• Cytogenetics: 22q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• MW: 61.4 kDa
• Summary: This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) lacks 2 in-frame exons in the coding region, as compared to variant 1. The encoded isoform 2 thus lacks an internal segment, as compared to isoform 1.