Pancreatic Lipase (PNLIP) (NM_000936) Human Untagged Clone

SKU SC300155

PNLIP (untagged)-Human pancreatic lipase (PNLIP)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Pancreatic Lipase
• Synonyms: PL; PNLIPD; PTL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC300155 representing NM_000936.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCTGCCACTTTGGACTCTTTCACTGCTGCTGGGAGCAGTAGCAGGAAAAGAAGTTTGCTACGAAAGA; CTCGGCTGCTTCAGTGATGACTCCCCATGGTCAGGAATTACGGAAAGACCCCTCCATATATTGCCTTGG; TCTCCAAAAGATGTCAACACCCGCTTCCTCCTATATACTAATGAGAACCCAAACAACTTTCAAGAAGTT; GCCGCAGATTCATCAAGCATCAGTGGCTCCAATTTCAAAACAAATAGAAAAACTCGCTTTATTATTCAT; GGATTCATAGACAAGGGAGAAGAAAACTGGCTGGCCAATGTGTGCAAGAATCTGTTCAAGGTGGAAAGT; GTGAACTGTATCTGTGTGGACTGGAAAGGTGGCTCCCGAACTGGATACACACAAGCCTCGCAGAACATC; AGGATCGTGGGAGCAGAAGTGGCATATTTTGTTGAATTTCTTCAGTCGGCGTTCGGTTACTCACCTTCC; AACGTGCATGTCATTGGCCACAGCCTGGGTGCCCACGCTGCTGGGGAGGCTGGAAGGAGAACCAATGGG; ACCATTGGACGCATCACAGGGTTGGACCCAGCAGAACCTTGCTTTCAGGGCACACCTGAATTAGTCCGA; TTGGACCCCAGCGATGCCAAATTTGTGGATGTAATTCACACGGATGGTGCCCCCATAGTCCCCAATTTG; GGGTTTGGAATGAGCCAAGTCGTGGGCCACCTAGATTTCTTTCCAAATGGAGGAGTGGAAATGCCTGGA; TGTAAAAAGAACATTCTCTCTCAGATTGTGGACATAGACGGAATCTGGGAAGGGACTCGAGACTTTGCG; GCCTGTAATCACTTAAGAAGCTACAAATATTACACTGATAGCATCGTCAACCCTGATGGCTTTGCTGGA; TTCCCCTGTGCCTCTTACAACGTCTTCACTGCAAACAAGTGTTTCCCTTGTCCAAGTGGAGGCTGCCCA; CAGATGGGTCACTATGCTGATAGATATCCTGGGAAAACAAATGATGTGGGCCAGAAATTTTATCTAGAC; ACTGGTGATGCCAGTAATTTTGCACGTTGGAGGTATAAGGTATCTGTCACACTGTCTGGAAAAAAGGTT; ACAGGACACATACTAGTTTCTTTGTTCGGAAATAAAGGAAACTCTAAGCAGTATGAAATTTTCAAGGGC; ACTCTCAAACCAGATAGTACTCATTCCAATGAATTTGACTCAGATGTGGATGTTGGGGACTTGCAGATG; GTTAAATTTATTTGGTATAACAATGTGATCAACCCAACTTTACCTAGAGTGGGAGCATCCAAGATTATA; GTGGAGACAAATGTTGGAAAACAGTTCAACTTCTGTAGTCCAGAAACCGTCAGGGAGGAAGTTCTGCTC; ACCCTCACACCGTGTTAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_000936
• Insert Size: 1398 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000936.3
• RefSeq Size: 1510 bp
• RefSeq ORF: 1398 bp
• Locus ID: 5406
• UniProt ID: P16233
• Cytogenetics: 10q25.3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways
• MW: 51.2 kDa
• Summary: This gene encodes a member of the lipase family of proteins. The encoded enzyme is secreted by the pancreas and hydrolyzes triglycerides in the small intestine, and is essential for the efficient digestion of dietary fats. Inhibition of the encoded enzyme may prevent high-fat diet-induced obesity in mice and result in weight loss in human patients with obesity. Mutations in this gene cause congenital pancreatic lipase deficiency, a rare disorder characterized by steatorrhea. [provided by RefSeq, Jul 2016]