Collagen IV (COL4A3) (NM_000091) Human Untagged Clone

SKU SC300008

COL4A3 (untagged)-Human collagen, type IV, alpha 3 (Goodpasture antigen) (COL4A3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Collagen IV
• Synonyms: ATS2; ATS3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC300008 representing NM_000091.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTA; CCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCC; ATGAGCGCCCGGACCGCCCCCAGGCCGCAGGTGCTCCTGCTGCCGCTCCTGCTGGTGCTCCTGGCGGCG; GCGCCCGCAGCCAGCAAGGGTTGTGTCTGTAAAGACAAAGGCCAGTGCTTCTGTGACGGGGCCAAAGGG; GAGAAGGGGGAGAAGGGCTTTCCTGGACCCCCCGGTTCTCCTGGCCAGAAAGGATTCACAGGTCCTGAA; GGCTTGCCTGGACCGCAGGGACCCAAGGGCTTTCCAGGACTTCCAGGACTCACGGGTTCCAAAGGTGTA; AGGGGAATAAGTGGATTGCCAGGATTTTCTGGTTCTCCTGGACTTCCAGGCACCCCAGGCAATACCGGG; CCTTACGGACTTGTCGGTGTACCAGGATGCAGTGGTTCTAAGGGTGAGCAGGGGTTTCCAGGACTCCCA; GGGACACTGGGCTACCCAGGGATCCCGGGTGCTGCTGGTTTGAAAGGACAAAAGGGTGCTCCTGCTAAA; GAAGAAGATATAGAACTTGATGCAAAAGGCGACCCCGGGTTGCCAGGGGCTCCAGGACCCCAGGGTTTG; CCAGGCCCTCCAGGTTTTCCTGGGCCTGTTGGCCCACCTGGTCCTCCGGGATTCTTTGGCTTTCCAGGA; GCCATGGGACCTAGAGGACCTAAGGGTCACATGGGTGAAAGAGTGATAGGACATAAAGGAGAGCGGGGT; GTGAAAGGGTTAACAGGACCCCCGGGACCACCAGGAACAGTTATTGTGACCCTAACTGGCCCAGATAAC; AGAACGGACCTCAAGGGGGAAAAGGGAGACAAGGGAGCAATGGGCGAGCCTGGACCTCCTGGACCCTCA; GGACTGCCTGGAGAATCATATGGATCTGAAAAGGGTGCTCCTGGAGACCCTGGCCTGCAGGGAAAACCC; GGAAAAGATGGTGTTCCTGGCTTCCCTGGAAGTGAGGGAGTCAAGGGCAACAGGGGTTTCCCTGGGTTA; ATGGGTGAAGATGGCATTAAGGGACAGAAAGGGGACATTGGCCCTCCAGGATTTCGTGGTCCAACAGAA; TATTATGACACATACCAGGAAAAGGGAGATGAAGGCACTCCAGGCCCACCAGGGCCCAGAGGAGCTCGT; GGCCCACAAGGTCCCAGTGGTCCCCCCGGAGTTCCTGGAAGTCCTGGATCATCAAGGCCTGGCCTCAGA; GGAGCCCCTGGATGGCCAGGCCTGAAAGGAAGTAAAGGGGAACGAGGCCGCCCAGGAAAGGATGCCATG; GGGACTCCTGGGTCCCCAGGTTGTGCTGGTTCACCAGGTCTTCCAGGATCACCGGGACCTCCAGGACCG; CCAGGTGACATCGTTTTTCGCAAGGGTCCACCTGGAGATCACGGACTGCCAGGCTATCTAGGGTCTCCA; GGAATCCCAGGAGTTGATGGGCCCAAAGGAGAACCAGGCCTCCTGTGTACACAGTGCCCTTATATCCCA; GGGCCTCCCGGTCTCCCAGGATTGCCAGGGTTACATGGTGTAAAAGGAATCCCAGGAAGACAAGGCGCA; GCTGGCTTGAAAGGAAGCCCAGGGTCCCCAGGAAATACAGGTCTTCCAGGATTTCCAGGTTTCCCAGGT; GCCCAGGGTGACCCAGGACTTAAAGGAGAAAAAGGTGAAACACTTCAGCCTGAGGGGCAAGTGGGTGTC; CCAGGTGACCCGGGGCTCAGAGGCCAACCTGGGAGAAAGGGCTTGGATGGAATTCCTGGAACTCCGGGA; GTGAAAGGATTACCAGGACCTAAAGGCGAACTGGCTCTGAGTGGTGAGAAAGGGGACCAAGGTCCTCCA; GGGGATCCTGGCTCCCCTGGGTCCCCAGGACCTGCAGGACCAGCTGGACCACCTGGCTACGGACCCCAA; GGAGAACCTGGTCTCCAGGGCACGCAAGGAGTTCCTGGAGCCCCCGGACCACCCGGAGAAGCCGGCCCT; AGGGGAGAGCTCAGTGTTTCAACACCAGTTCCAGGCCCACCAGGACCTCCAGGGCCCCCTGGCCATCCT; GGCCCCCAAGGTCCACCTGGTATCCCTGGATCCCTGGGGAAATGTGGAGATCCTGGTCTTCCAGGGCCT; GATGGTGAACCAGGAATTCCAGGAATTGGATTTCCTGGGCCTCCTGGACCTAAGGGAGACCAAGGTTTT; CCAGGTACAAAAGGATCACTGGGTTGTCCTGGAAAAATGGGAGAGCCTGGGTTACCTGGAAAGCCAGGC; CTCCCAGGAGCCAAGGGAGAACCAGCAGTAGCCATGCCTGGAGGACCAGGAACACCAGGTTTTCCAGGA; GAAAGAGGCAATTCTGGGGAACATGGAGAAATTGGACTCCCTGGACTTCCAGGTCTCCCTGGAACTCCA; GGAAATGAAGGGCTTGATGGACCACGAGGAGATCCAGGGCAGCCTGGACCACCTGGAGAACAAGGACCC; CCAGGAAGGTGCATAGAGGGTCCCAGGGGAGCCCAAGGACTTCCAGGCTTAAATGGATTGAAAGGGCAA; CAAGGCAGAAGAGGTAAAACGGGGCCAAAGGGAGACCCAGGAATTCCAGGCTTGGATAGATCAGGATTT; CCTGGAGAAACTGGATCACCAGGAATTCCAGGTCATCAAGGTGAAATGGGACCACTGGGTCAAAGAGGA; TATCCAGGAAATCCGGGAATTTTAGGGCCACCAGGTGAAGATGGAGTGATTGGGATGATGGGCTTTCCT; GGAGCCATTGGCCCTCCAGGGCCCCCTGGGAACCCAGGCACACCAGGGCAGAGGGGGAGCCCTGGAATT; CCAGGAGTAAAGGGCCAGAGAGGAACCCCAGGAGCCAAGGGGGAACAAGGAGATAAAGGAAATCCCGGG; CCTTCAGAGATATCCCACGTAATAGGGGACAAAGGAGAACCAGGTCTCAAAGGATTCGCAGGAAATCCA; GGTGAGAAAGGAAACAGAGGCGTTCCAGGGATGCCAGGTTTAAAGGGCCTCAAAGGACTACCCGGACCA; GCAGGACCACCAGGCCCCAGAGGAGATTTGGGCAGCACTGGGAATCCTGGAGAACCAGGACTGCGTGGT; ATACCAGGAAGCATGGGGAACATGGGCATGCCAGGTTCTAAAGGAAAAAGGGGAACTTTGGGATTCCCA; GGTCGAGCAGGAAGACCAGGCCTCCCAGGTATTCATGGTCTCCAGGGAGATAAGGGAGAGCCAGGTTAT; TCAGAAGGTACAAGGCCAGGACCACCGGGACCAACGGGGGATCCAGGACTGCCGGGTGATATGGGAAAG; AAAGGAGAAATGGGGCAACCTGGCCCACCTGGACATTTGGGGCCTGCTGGACCTGAGGGAGCCCCTGGA; AGTCCTGGAAGTCCTGGCCTCCCAGGAAAGCCAGGTCCTCATGGTGATTTGGGTTTTAAAGGAATCAAA; GGCCTCCTGGGCCCTCCAGGAATCAGAGGCCCTCCAGGTCTTCCAGGATTTCCAGGATCTCCTGGACCA; ATGGGTATAAGAGGTGACCAAGGACGTGATGGAATTCCTGGTCCAGCCGGAGAAAAGGGAGAAACGGGT; TTATTGAGGGCCCCTCCAGGCCCAAGAGGGAACCCTGGTGCTCAAGGAGCCAAAGGAGACAGGGGAGCC; CCAGGTTTTCCTGGCCTCCCGGGCAGAAAAGGGGCCATGGGAGATGCTGGACCTCGAGGACCCACAGGC; ATAGAAGGATTCCCAGGGCCACCAGGTCTGCCCGGTGCAATTATCCCTGGCCAGACAGGAAATCGTGGT; CCACCAGGCTCAAGAGGAAGCCCAGGTGCGCCTGGTCCCCCTGGACCTCCAGGGAGTCATGTAATAGGC; ATAAAAGGAGACAAAGGGTCTATGGGCCACCCTGGCCCAAAAGGTCCACCTGGAACTGCAGGAGACATG; GGACCACCAGGTCGTCTGGGAGCACCAGGTACTCCAGGTCTTCCAGGACCCAGAGGTGATCCTGGATTC; CAGGGGTTTCCAGGCGTGAAAGGAGAAAAGGGTAATCCTGGATTTCTAGGATCCATTGGACCTCCAGGA; CCAATTGGGCCAAAAGGACCACCTGGTGTACGTGGAGACCCTGGCACACTTAAGATTATCTCCCTTCCA; GGAAGCCCAGGGCCACCTGGCACACCTGGAGAACCAGGGATGCAGGGAGAACCTGGGCCACCAGGGCCA; CCTGGAAACCTAGGACCCTGTGGGCCAAGAGGTAAGCCAGGCAAGGATGGAAAACCAGGAACTCCTGGA; CCAGCTGGAGAAAAAGGCAACAAAGGTTCTAAAGGAGAGCCAGGACCAGCTGGATCAGATGGATTGCCA; GGTTTGAAAGGAAAACGTGGAGACAGTGGATCACCTGCAACCTGGACAACGAGAGGCTTTGTCTTCACC; CGACACAGTCAAACCACAGCAATTCCTTCATGTCCAGAGGGGACAGTGCCACTCTACAGTGGGTTTTCT; TTTCTTTTTGTACAAGGAAATCAACGAGCCCACGGACAAGACCTTGGAACTCTTGGCAGCTGCCTGCAG; CGATTTACCACAATGCCATTCTTATTCTGCAATGTCAATGATGTATGTAATTTTGCATCTCGAAATGAT; TATTCATACTGGCTGTCAACACCAGCTCTGATGCCAATGAACATGGCTCCCATTACTGGCAGAGCCCTT; GAGCCTTATATAAGCAGATGCACTGTTTGTGAAGGTCCTGCGATCGCCATAGCCGTTCACAGCCAAACC; ACTGACATTCCTCCATGTCCTCACGGCTGGATTTCTCTCTGGAAAGGATTTTCATTCATCATGTTCACA; AGTGCAGGTTCTGAGGGCACCGGGCAAGCACTGGCCTCCCCTGGCTCCTGCCTGGAAGAATTCCGAGCC; AGCCCATTTCTAGAATGTCATGGAAGAGGAACGTGCAACTACTATTCAAATTCCTACAGTTTCTGGCTG; GCTTCATTAAACCCAGAAAGAATGTTCAGAAAGCCTATTCCATCAACTGTGAAAGCTGGGGAATTAGAA; AAAATAATAAGTCGCTGTCAGGTGTGCATGAAGAAAAGACACTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: AscI-MluI
• ACCN: NM_000091
• Insert Size: 5013 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000091.4
• RefSeq Size: 8114 bp
• RefSeq ORF: 5013 bp
• Locus ID: 1285
• UniProt ID: Q01955
• Cytogenetics: 2q36.3
• Protein Families: Druggable Genome
• MW: 161.8 kDa
• Summary: Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010]; Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.