PDP1 (NM_018444) Human Untagged Clone

SKU SC128049

PDP1 (untagged)-Human pyruvate dehyrogenase phosphatase catalytic subunit 1 (PDP1), nuclear gene encoding mitochondrial protein, transcript variant 5

Product Data

• Type: Human Untagged Clone
• Target Symbol: PDP1
• Synonyms: PDH; PDP; PDPC; PPM2A; PPM2C
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128049 sequence for NM_018444 edited (data generated by NextGen Sequencing); ATGCCAGCACCAACTCAACTGTTTTTTCCTCTCATCCGTAACTGTGAACTGAGCAGGATC; TATGGCACTGCATGTTACTGCCACCACAAACATCTCTGTTGTTCCTCATCGTACATTCCT; CAGAGTCGACTGAGATACACACCTCATCCAGCATATGCTACCTTTTGCAGGCCAAAGGAG; AACTGGTGGCAGTACACCCAAGGAAGGAGATATGCTTCCACACCACAGAAATTTTACCTC; ACACCTCCACAAGTCAATAGCATCCTTAAAGCTAATGAATACAGTTTCAAAGTGCCAGAA; TTTGACGGCAAAAATGTCAGTTCTATCCTTGGATTTGACAGCAATCAGCTGCCTGCAAAT; GCACCCATTGAGGACCGGAGAAGTGCAGCAACCTGCTTGCAGACCAGAGGGATGCTTTTG; GGGGTTTTTGATGGCCATGCAGGTTGTGCTTGTTCCCAGGCAGTCAGTGAAAGACTCTTT; TATTATATTGCTGTCTCTTTGTTACCCCATGAGACTTTGCTAGAGATTGAAAATGCAGTG; GAGAGCGGCCGGGCACTGCTACCCATTCTCCAGTGGCACAAGCACCCCAATGATTACTTT; AGTAAGGAGGCATCCAAATTGTACTTTAACAGCTTGAGGACTTACTGGCAAGAGCTTATA; GACCTCAACACTGGTGAGTCGACTGATATTGATGTTAAGGAGGCTCTAATTAATGCCTTC; AAGAGGCTTGATAATGACATCTCCTTGGAGGCGCAAGTTGGTGATCCTAATTCTTTTCTC; AACTACCTGGTGCTTCGAGTGGCATTTTCTGGAGCCACTGCTTGTGTGGCCCATGTGGAT; GGTGTTGACCTTCATGTGGCCAATACTGGCGATAGCAGAGCCATGCTGGGTGTGCAGGAA; GAGGACGGCTCATGGTCAGCAGTCACGCTGTCTAATGACCACAATGCTCAAAATGAAAGA; GAACTAGAACGGCTGAAATTGGAACATCCAAAGAGTGAGGCCAAGAGTGTCGTGAAACAG; GATCGGCTGCTTGGCTTGCTGATGCCATTTAGGGCATTTGGAGATGTAAAGTTCAAATGG; AGCATTGACCTTCAAAAGAGAGTGATAGAATCTGGCCCAGACCAGTTGAATGACAATGAA; TATACCAAGTTTATTCCTCCTAATTATCACACACCTCCTTATCTCACTGCTGAGCCAGAG; GTAACTTACCACCGATTAAGGCCACAGGATAAGTTTCTGGTGTTGGCTACTGATGGGTTG; TGGGAGACTATGCATAGGCAGGATGTGGTTAGGATTGTGGGTGAGTACCTAACTGGCATG; CATCACCAACAGCCAATAGCTGTTGGTGGCTACAAGGTGACTCTGGGACAGATGCATGGC; CTTTTAACAGAAAGGAGAACCAAAATGTCCTCGGTATTTGAGGATCAGAACGCAGCAACC; CATCTCATTCGCCACGCTGTGGGCAACAACGAGTTTGGGACTGTTGATCATGAGCGCCTC; TCTAAAATGCTTAGTCTTCCTGAAGAGCTTGCTCGAATGTACAGAGATGACATTACAATC; ATTGTAGTTCAGTTCAATTCTCATGTTGTAGGGGCGTATCAAAACCAAGAATAG; ; Clone variation with respect to NM_018444.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018444 unedited; NTTGTTCGGATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCGGGA; AGCACTCCGGCCCGAACGTGCGGGCGGGGCAGGGTGGCGGCCCCGCACGGTAGGGGAGCA; GAGTGGGCAGGCCGGGGGTGAGGGCTCGCGCTCCGGGAGCTGCACGGGGCTGCGTGGAAA; GAGCGCCGAGCGGTGGCGTCGTTGTCGCCCCCTCCTCGTCGGGAAGAATCGTTTGGTCTC; CTGCCGTGCCCGGAATCCCAGTCAGAAGTTCCAGCCTGCCACTGTTCTCTGATGCCATGC; CAGCACCAACTCAACTGTTTTTTCCTCTCATCCGTAACTGTGAACTGAGCAGGATCTATG; GCACTGCATGTTACTGCCACCACAAACATCTCTGTTGTTCCTCATCGTACATTCCTCAGA; GTCGACTGAGATACACACCTCATCCAGCATATGCTACCTTTTGCAGGCCAAAGGAGAACT; GGTGGCAGTACACCCAAGGAAGGAGATATGCTTCCACACCACAGAAATTTTACCTCACAC; CTCCACAAGTCAATAGCATCCTTAAAGCTAATGAATACAGTTTCAAAGTGCCAGAATTTG; ACGGCAAAAATGTCAGTTCTATCCTTGGATTTGACAGCAATCAGCTGCCTGCAAATGCAC; CCATTGAGGACCGGAGAAGTGCAGCAACCTGCTTGCAGACCAGAGGGATGCTTTTGGGGG; TTTTTGATGGCCATGCANGTTGTGCTTGTTCCCAGCAGTCAGTGAAAGACTCTTTTATTA; TATTGCTGTCTCTTTGTTACCCCATGAGACTTTGCTAGAGATTGAAAATGCAGTGGAGAG; CGGCCGGGCACTGCTACCCATTCTNCAGTGGCACAAGCACCCCATGATTACTTTAGTAAG; GNAGCATTCAAATGTACTTTACAGCTTGAGGACTACTGGCAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_018444 unedited; TTTGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAAGTTTACATGT; ATTCTTTAATTCTAGACACCGTACAACAGTGACAACCAATTACAATAAAATCACAATTGC; TTTTAGATGACAGTACTTTCAGATTTCTAATACCCAATTACTTTCATTTCCACAATGTCA; ACTTCATGCTGCATTTTCATTTCTATAGAGCAGACAAGCTTCCAGACTGCAGACCAAGTT; TCTTGTGTAATAATACTACTATCTTGATCATGACCACAGGAAACCAATTTTATATTCCCT; GTACTATAGAGATGAGACATTATTTGGTGTATATGAAACTCTTCAGTGGTGGTGTTCAAG; AATATTCAAATAGTAGCTGAAAATAGGGTTTGCTAGAGCAGTCCACATATTTCATTAAAA; GAAAAATGCCCAGTCAAAACATTTAGAAATAAATATATAGTACAGCCTTTCCCTCTCCCA; AATACTACCCAGGAAAAAAGTTCCAAGCTGACTTAAAAAAAAAAAAAACACACAAATAAA; AACAAAAACACCTCAAAAAATAAACATTATATGATATTCTGAGCAGCAATCTTCCTGAGT; CTATTTTCAATTGAAACAAATTCTGCAGCCAATAGTACACTGATTCAGTATCCACAGTCC; AGAAGGAGTTCTTGAACATCTAGTTCATATCCCCAAAAAATGTGAAAAGACTCCTAAACA; CCCAAGTTATTACACAATGGNGAAGCTGATATTTGGCTTGTATTAAAATCCAGGTGAATA; TCANACATAATCACAGCCCTTACAGTGATGCTAGAATAGTGATGACAATATGTGCACAGC; TGTNTACAGATCACTATTAACACCAGGACAAATGCCCCTTGATGCTATCAAAAGCCCAAT; TGATATATTATTTCATTACATGATCAGNATAGAAGGACTGATATATATGGGGACGCTGCA; ACATTGATGCCCTAGTTTCTATAAGCTCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_018444
• Insert Size: 4260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_018444.2, NP_060914.2
• RefSeq Size: 4215 bp
• RefSeq ORF: 1614 bp
• Locus ID: 54704
• UniProt ID: Q9P0J1
• Cytogenetics: 8q22.1
• Domains: PP2C
• Protein Families: Druggable Genome, Phosphatase
• Summary: Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]; Transcript Variant: This variant (5) lacks an internal segment, compared to variant 2. The difference causes translation initiation at a downstream AUG and results in an isoform (3) with a shorter N-terminus, compared to isoform 2.