Lipoamide Dehydrogenase (DLD) (NM_000108) Human Untagged Clone

SKU SC126940

DLD (untagged)-Human dihydrolipoamide dehydrogenase (DLD)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Lipoamide Dehydrogenase
• Synonyms: DLDD; DLDH; E3; GCSL; LAD; OGDC-E3; PHE3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126940 sequence for NM_000108 edited (data generated by NextGen Sequencing); ATGCAGAGCTGGAGTCGTGTGTACTGCTCCTTGGCCAAGAGAGGCCATTTCAATCGAATA; TCTCATGGCCTACAGGGACTTTCTGCAGTGCCTCTGAGAACTTACGCAGATCAGCCGATT; GATGCTGATGTAACAGTTATAGGTTCTGGTCCTGGAGGATATGTTGCTGCTATTAAAGCT; GCCCAGTTAGGCTTCAAGACAGTCTGCATTGAGAAAAATGAAACACTTGGTGGAACATGC; TTGAATGTTGGTTGTATTCCTTCTAAGGCTTTATTGAACAACTCTCATTATTACCATATG; GCCCATGGAAAAGATTTTGCATCTAGAGGAATTGAAATGTCCGAAGTTCGCTTGAATTTA; GACAAGATGATGGAGCAGAAGAGTACTGCAGTAAAAGCTTTAACAGGTGGAATTGCCCAC; TTATTCAAACAGAATAAGGTTGTTCATGTCAATGGATATGGAAAGATAACTGGCAAAAAT; CAAGTCACTGCTACGAAAGCTGATGGCGGCACTCAGGTTATTGATACAAAGAACATTCTT; ATAGCCACGGGTTCAGAAGTTACTCCTTTTCCTGGAATCACGATAGATGAAGATACAATA; GTGTCATCTACAGGTGCTTTATCTTTAAAAAAAGTTCCAGAAAAGATGGTTGTTATTGGT; GCAGGAGTAATAGGTGTAGAATTGGGTTCAGTTTGGCAAAGACTTGGTGCAGATGTGACA; GCAGTTGAATTTTTAGGTCATGTAGGTGGAGTTGGAATTGATATGGAGATATCTAAAAAC; TTTCAACGCATCCTTCAAAAACAGGGGTTTAAATTTAAATTGAATACAAAGGTTACTGGT; GCTACCAAGAAGTCAGATGGAAAAATTGATGTTTCTATTGAAGCTGCTTCTGGTGGTAAA; GCTGAAGTTATCACTTGTGATGTACTCTTGGTTTGCATTGGCCGACGACCCTTTACTAAG; AATTTGGGACTAGAAGAGCTGGGAATTGAACTAGATCCCAGAGGTAGAATTCCAGTCAAT; ACCAGATTTCAAACTAAAATTCCAAATATCTATGCCATTGGTGATGTAGTTGCTGGTCCA; ATGCTGGCTCACAAAGCAGAGGATGAAGGCATTATCTGTGTTGAAGGAATGGCTGGTGGT; GCTGTGCACATTGACTACAATTGTGTGCCATCAGTGATTTACACACACCCTGAAGTTGCT; TGGGTTGGCAAATCAGAAGAGCAGTTGAAAGAAGAGGGTATTGAGTACAAAGTTGGGAAA; TTCCCATTTGCTGCTAACAGCAGAGCTAAGACAAATGCTGACACAGATGGCATGGTGAAG; ATCCTTGGGCAGAAATCGACAGACAGAGTACTGGGAGCACATATTCTTGGACCAGGTGCT; GGAGAAATGGTAAATGAAGCTGCTCTTGCTTTGGAATATGGAGCATCCTGTGAAGATATA; GCTAGAGTCTGTCATGCACATCCGACCTTATCAGAAGCTTTTAGAGAAGCAAATCTTGCT; GCGTCATTTGGCAAATCAATCAACTTTTGA; ; Clone variation with respect to NM_000108.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000108 unedited; TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCCCAGCGGAGGT; GAAAGTTTGGCGGAAAGGAAAAACAGCGGAAAAAGCAGAGCTGGAGTCGTGTGTACTGCT; CCTTGGCCAAGAGAGGCCATTTCAATCGAATATCTCATGGCCTACAGGGACTTTCTGCAG; TGCCTCTGAGAACTTACGCAGATCAGCCGATTGATGCTGATGTAACAGTTATAGGTTCTG; GTCCTGGAGGATATGTTGCTGCTATTAAAGCTGCCCAGTTAGGCTTCAAGACAGTCTGCA; TTGAGAAAAATGAAACACTTGGTGGAACATGCTTGAATGTTGGTTGTATTCCTTCTAAGG; CTTTATTGAACAACTCTCATTATTACCATATGGCCCATGGAAAAGATTTTGCATCTAGAG; GAATTGAAATGTCCGAAGTTCGCTTGAATTTAGACAAGATGATGGAGCAGAAGAGTACTG; CAGTAAAAGCTTTAACAGGTGGAATTGCCCACTTATTCAAACAGAATAAGGTTGTTCATG; TCAATGGATATGGAAAGATAACTGGCAAAAATCAAGTCACTGCTACGAAAGCTGATGGCG; GCACTCAGGTTATTGATACAAAGAACATTCTTATAGCCACGGGTTCAGAAGTTACTCCTT; TTCCTGGAATCACGATAGATGAAGATACAATAGTGTCATCTACAGGTGCTTTATCTTTAA; AAAAAGTTCCAGAAAAGATGGTTGTTATTGGTGCAGGAGTAATAGGTGTAGAAATTGGGT; TCAGTTTGGCAAAGACTTGGTGCAGATGTGACAGCAGTTGAATTTTTAGGTCATGTAGGT; GGGAGTGGNAATGATATGGNAGATATCTAAAAACTTCACN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000108 unedited; TTTTTTTTTTTTTTTTTAACCAGTAAATGAATATTTAAGACGTTTATTTCAGTATCTTCA; TTCCCCACTGTAATACATTTGAATGCCCACGTATTTGAGTTCCGTAAATAGACTCTCTTA; ATTTGGGTAACTATATAATTACCCTTCAGAGTTGCAGACAACAATGGAAGCTTTAAAACC; TCTTCAACACAAATGCTACCCCTAAAATGAAAGAATTTAGAGGTTAAATAAAACAAGTGA; GAGACCATTTACTTACATCAGTTCGGTTTATAGACATTTGAATCATATCTGAATGACTGA; CTTGTTTCCAATGTGAAAACCAAATTAAAAATAACTTGATCACTGTGCTTCAAACACAAC; TGTTCACATATCAAATTCTAGCTCCAGCCATGTAAAATTCAACTTTTCTGTATCAGAGAA; AAAAGCTATTTAAATTCAGGGGCATTGAATCTTTCATGAACAGCATGAAAATATGAGTTG; ACGGAGATATTCCAGGATGCTACAATAAGTAGCTTTTTGTCTTACATATTAGTGCACTGA; AACAAAATACTAAATTTATGTAAAATATAGATATAAGAGTATTCCATTTTGTCCAAACCT; ATTAAATACCTTCCAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_000108
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000108.2, NP_000099.1
• RefSeq Size: 2320 bp
• RefSeq ORF: 1530 bp
• Locus ID: 1738
• UniProt ID: P09622
• Cytogenetics: 7q31.1
• Domains: pyr_redox, pyr_redox_dim
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Glycine, Glycolysis / Gluconeogenesis, leucine and isoleucine degradation, Metabolic pathways, Pyruvate metabolism, serine and threonine metabolism, Valine
• Summary: This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.