DARPP32 (PPP1R1B) (NM_032192) Human Untagged Clone

SKU SC126726

PPP1R1B (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 1B (PPP1R1B), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: DARPP32
• Synonyms: DARPP-32; DARPP32
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032192 edited; CCGCCGCCTGCGCGGGGGAGCCCAGCACAGACCGCCGCCGGGACCCCGAGTCGCGCACCC; CAGCCCCACCGCCCACCCCGCGCGCCATGGACCCCAAGGACCGCAAGAAGATCCAGTTCT; CGGTGCCCGCGCCCCCTAGCCAGCTCGACCCCCGCCAGGTGGAGATGATCCGGCGCAGGA; GACCAACGCCTGCCATGCTGTTCCGGCTCTCAGAGCACTCCTCACCAGAGGAGGAAGCCT; CCCCCCACCAGAGAGCCTCAGGAGAGGGGCACCATCTCAAGTCGAAGAGACCCAACCCCT; GTGCCTACACACCACCTTCGCTGAAAGCTGTGCAGCGCATTGCTGAGTCTCACCTGCAGT; CTATCAGCAATTTGAATGAGAACCAGGCCTCAGAGGAGGAGGATGAGCTGGGGGAGCTTC; GGGAGCTGGGTTATCCAAGAGAGGAAGATGAGGAGGAAGAGGAGGATGATGAAGAAGAGG; AAGAAGAAGAGGACAGCCAGGCTGAAGTCCTGAAGGTCATCAGGCAGTCTGCTGGGCAAA; AGACAACCTGTGGCCAGGGTCTGGAAGGGCCCTGGGAGCGCCCACCCCCTCTGGATGAGT; CCGAGAGAGATGGAGGCTCTGAGGACCAAGTGGAAGACCCAGCACTAAGTGAGCCTGGGG; AGGAACCTCAGCGCCCTTCCCCCTCTGAGCCTGGCACATAGGCACCCAGCCTGCATCTCC; CAGGAGGAAGTGGAGGGGACATCGCTGTTCCCCAGAAACCCACTCTATCCTCACCCTGTT; TTGTGCTCTTCCCCTCGCCTGCTAGGGCTGCGGCTTCTGACTTCTAGAAGACTAAGGCTG; GTCTGTGTTTGCTTGTTTGCCCACCTTTGGCTGATACCCAGAGAACCTGGGCACTTGCTG; CCTGATGCCCACCCCTGCCAGTCATTCCTCCATTCACCCAGCGGGAGGTGGGATGTGAGA; CAGCCCACATTGGAAAATCCAGAAAACCGGGAACAGGGATTTGCCCTTCACAATTCTACT; CCCCAGATCCTCTCCCCTGGACACAGGAGACCCACAGGGCAGGACCCTAAGATCTGGGGA; AAGGAGGTCCTGAGAACCTTGAGGTACCCTTAGATCCTTTTCTACCCACTTTCCTATGGA; GGATTCCAAGTCACCACTTCTCTCACCGGCTTCTACCAGGGTCCAGGACTAAGGCGTTTT; TCTCCATAGCCTCAACATTTTGGGAATCTTCCCTTAATCACCCTTGCTCCTCCTGGGTGC; CTGGAAGATGGACTGGCAGAGACCTCTTTGTTGCGTTTTGTGCTTTGATGCCAGGAATGC; CGCCTAGTTTATGTCCCCGGTGGGGCACACAGCGGGGGGCGCCAGGTTTTCCTTGTCCCC; CAGCTGCTCTGCCCCTTTCCCCTTCTTCCCTGACTCCAGGCCTGAACCCCTCCCGTGCTG; TAATAAATCTTTGTAAATAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032192 unedited; GCGCGAATTCGGCACCAGCCGCCGCCTGCGCGGGGGAGCCCAGCACAGACCGCCGCCGGG; ACCCCGAGTCGCGCACCCCAGCCCCACCGCCCACCCCGCGCGCCATGGACCCCAAGGACC; GCAAGAAGATCCAGTTCTCGGTGCCCGCGCCCCCTAGCCAGCTCGACCCCCGCCAGGTGG; AGATGATCCGGCGCAGGTACCAACGCCGGGATGCTGTTCCGGCTCTCAGAGCACTCCTCA; CCAGAGGAGGAAGCCTCCCCCCACCAGAGAGCCTCAGGAGAGGGGCACCATCTCAAGTCG; AAGAGACCCAACCCCTGTGCCTACACACCACCTTCGCTGAAAGCTGTGCAGCGCATTGCT; GAGTCTCACCTGCAGTCTATCAGCAATTTGAATGAGAACCAGGCCTCANAGGAGGAGGAT; GAGCTGGGGGAGCTTCGGGAGCTGGGTTATCCAAGAGAGGAAGATGAGGAGGAAGAGGAG; GATGATGAAAAAGAGGAAGAAGAAGAGGACAGCCAGGCTGAAGTCCTGAAGGTCATCAGG; CAGTCTGCTGGGCAAAAGACAACCTGTGGCCAGGGTCTGGAAGGGCCCTGGGAGCGCCCA; CCCCCTCTGGATGAGTCCGAGAGAGATGGAGGCTCTGAGGACCAAGTGGAAGACCCCAGC; ACTAGTGAGCCTGGGGAGGAACCTCAGCGCCCTTCCCCCCTCTGAGCCTGGCACATANGC; ACCCAGCCTGCATCTNCCAGAGGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032192 unedited; GCTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTATTTACAAA; GATTTATTACAGCACGGGAGGGGTTCAGGCCTGGAGTCAGGGAAGAAGGGGAAAGGGGCA; GAGCAGCTGGGGGACAAGGAAAACCTGGCGCCCCCCGCTGTGTGCCCCACCGGGGACATA; AACTAGGCGGCATTCCTGGCATCAAAGCACAAAACGCAACAAAGAGGTCTCTGCCAGTCC; ATCTTCCAGGCACCCAGGAGGAGCAAGGGTGATTAAGGGAAGATTCCCAAAATGTTGAGG; CTATGGAGAAAAACGCCTTAGTCCTGGACCCTGGTAGAAGCCGGTGAGAGAAGTGGTGAC; TTGGAATCCTCCATAGGAAAGTGGGTAGAAAAGGATCTAAGGGTACCTCAAGGTTCTCAG; GACCTCCTTTCCCCAGATCTTAGGGTCCTGCCCTGTGGGTCTCCTGTGTCCAGGGAGAGG; ATCTGGGGAGTANAAAATGTGAAGGGCAAATCCCTGTTCCCCGTTTTCTGGGATTTTCAA; ATGTGGGCTGTCTCACATNCCACCTNCCGCCTGGGTGAATGGAGGAACAACTGGCANGGG; GTGGGCATCAAGCAGCAAGTGCCCAGGTTCTCTTGGGTATCAGCCAAAAGTGGGGAAACA; AGCAAACACAGACAACCTTAATCTTTTAAAAGTCAAAGCCGCAGCCCTACCGGCGAGGGG; AAGAGCCCAACCCGGTAGATAAAAAGGGGTTTTTTGGGAACCAACAAGTCCCCTCCCTTC; TCCTGGGAATGCACGCTGGTGCCTTTTGCACCCTAAGGGGGAGGGCCCCGGGGTCTCCCC; GCCCATTAAGGGGGGGTTTCCTTTGCCTAAAGCCCCTTTTTTTGCTTCCCAGGGGGGGGG; GGTTCCGGGCTTTCAACCTGCCCAGGTNTTTTTCCCAAAGCCGGGACCTAAAATANCCGG; GGGCCCTTTTTTCTTTTAAACCCCCTCTCCCCTGGTCTGGACA;
• Restriction Sites: NotI-NotI
• ACCN: NM_032192
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_032192.2.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_032192.2, NP_115568.2
• RefSeq Size: 1841 bp
• RefSeq ORF: 615 bp
• Locus ID: 84152
• UniProt ID: Q9UD71
• Cytogenetics: 17q12
• Protein Families: Druggable Genome
• Summary: This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).