ARHI (DIRAS3) (NM_004675) Human Untagged Clone

SKU SC125693

DIRAS3 (untagged)-Human DIRAS family, GTP-binding RAS-like 3 (DIRAS3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ARHI
• Synonyms: ARHI; NOEY2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004675 edited; GGGTGTCCAGTTGGTTGCCGCGGCAGTCTCTCCGAGCAGCGCATTTGTCTTCTAGGCTGC; TTGGTTCGTGCCTCCGAGAAAGGGGTCTCCTGCTGCCAGCTAAGTGTGGGAGAACTTGTG; TACGTATCTCCCCTCCGAATCCCAACGATGGGTAACGCCAGCTTTGGCTCCAAGGAACAG; AAGCTGCTGAAGCGGTTGCGGCTTCTGCCCGCCCTGCTTATCCTCCGCGCCTTCAAGCCC; CACAGGAAGATCAGAGATTACCGCGTCGTGGTAGTCGGCACCGCTGGTGTGGGGAAAAGT; ACGCTGCTGCACAAGTGGGCGAGCGGCAACTTCCGTCATGAGTACCTGCCGACCATTGAA; AATACCTACTGCCAGTTGCTGGGCTGCAGCCACGGTGTGCTTTCCCTGCACATCACCGAC; AGCAAGAGTGGCGACGGCAACCGCGCTCTGCAGCGCCACGTTATAGCCCGGGGCCACGCC; TTCGTCCTGGTCTACTCAGTCACCAAGAAGGAAACCCTGGAAGAGCTGAAGGCCTTCTAT; GAGCTGATCTGCAAGATCAAAGGTAACAACCTGCATAAGTTCCCCATCGTGCTGGTGGGC; AATAAAAGTGATGACACCCACCGGGAGGTGGCCCTGAATGATGGTGCCACCTGTGCGATG; GAGTGGAATTGCGCCTTCATGGAGATTTCAGCCAAGACCGATGTGAATGTGCAGGAGCTG; TTCCACATGCTGCTGAATTACAAGAAAAAGCCCACCACCGGCCTCCAGGAGCCCGAGAAG; AAATCCCAGATGCCCAACACCACTGAGAAGCTGCTTGACAAGTGCATAATCATGTGAGCC; CTGGGCCTTAAGAGCCAGCTCTTCCTATCCTGTAGCGTGTAGAAAACGTGGACTCATTTC; ACTATGTTACATGTACATGGTTGATTTTGTGCTGTTGTTTGGACTGTAACATCCATGTTG; TCAATACGTATACCTTGTAAGTGGATAACTTTTCTTTTTCCCAGGCCAGAGAATTCAAAT; TGTTAAAACATTGGCATTTGAAGAGGAGAACAAAATGTAGCATGATGTATTTAAAGTAAG; GCCTTTAGTAATGAATGTAATGAGAGAAAATGTTTTGAAAAGAACAAAACATCAAAATGA; ATAGAAAGAAAAATTGGAAGGCGTCCTTTTGGTAACCCGATTATTGTGTATTACCTTTAA; ATATTTCACATCCTGTAAGTGCTTAATCATATCTTTTAATTGTGTATTTAAGAAAAGTGT; TTTCACAACAAAAGCTTTTGATAAATTGCTGCGTGACATATACTAAATAAAAAAATGAAT; ATGTTGATCATTAGGGGTGTGGGAGCAGAGAAAATTGTGAAAGTGACTCTCACTAAAGAT; GTTAGTAGTTTCTCATGTCATTTAAAAATGTTTGAGTATTCTGCATAGCAGTTTGTAAAA; GTGTAACAGCTTATTGACTTAATAAAGCTTTTCCTGCATGCAGAAAAAAAAAAAAAAAAA; AAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004675 unedited; NAAGGTTCANGATTTGTNATACGACTCATATAGGCGGCCGCGATTCGGCCTTACGGCCGG; GGGGTGTCCAGTTGGTTGCCGCGGCAGTCTCTCCGAGCAGCGCATTTGTCTTCTAGGCTG; CTTGGTTCGTGCCTCCGAGAAAGGGTCTCCTGCTGCCAGCTAAGTGTGGGAGAACTTGTG; TACGTATCTCCCCTCCGAATCCCAACGATGGGTAACGCCAGCTTTGGCTCCAAGGAACAG; AAGCTGCTGAAGCGGTTGCGGCTTCTGCCCGCCCTGCTTATCCTCCGCGCCTTCAAGCCC; CACAGGAAGATCAGAGATTACCGCGTCGTGGTAGTCGGCACCGCTGGTGTGGGGAAAAGT; ACGCTGCTGCACAAGTGGGCGAGCGGCAACTTCCGTCATGAGTACCTGCCGACCATTGAA; AATACCTACTGCCAGTTGCTGGGCTGCAGCCACGGTGTGCTTTCCCTGCACATCACCGAC; AGCAAGAGTGGCGACGGCAACCGCGCTCTGCAGCGCCACGTTATAGCCCGGGGCCACGCC; TTCGTCCTGGTCTACTCAGTCACCAAGAAGGAAACCCTGGAAGAGCTGAAGGCCTTCTAT; GAGCTGATCTGCAAGATCAAAGGTAACAACCTGCATAAGTTCCCCATCGTGCTGGTGGGN; CATAAAAGTGATGACACCCACCGGGAGGTGGCCCTGAATGATGGTGCCACCTGTGCGATG; GAGTGGAATTGCGCCTTCATGGAGATTTCAGCCAAGACCGATGTGAATGTGCANGAGCTG; TTCCACATGCTGCTGAATTTACAGAAAAGCCCACCACCGGCCCTCCAGAGCCCCGAGAGA; ATCCCAGATGCCCAACACCACTGAGAAGCTGCTTGACAAGTGCN;
• Restriction Sites: NotI-NotI
• ACCN: NM_004675
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004675.2, NP_004666.1
• RefSeq Size: 1642 bp
• RefSeq ORF: 690 bp
• Locus ID: 9077
• UniProt ID: O95661
• Cytogenetics: 1p31.3
• Summary: This gene encodes a member of the ras superfamily. This gene is imprinted gene with monoallelic expression of the paternal allele which is associated with growth suppression. The encoded protein acts as a tumor suppressor whose function is abrogated in many ovarian and breast cancers. This protein may also play a role autophagy in certain cancer cells by regulating the autophagosome initiation complex. [provided by RefSeq, Nov 2015]