PRKACA (NM_002730) Human Untagged Clone

CAT#: SC125607

PRKACA (untagged)-Human protein kinase, cAMP-dependent, catalytic, alpha (PRKACA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKACA
• Synonyms: CAFD1; PKACA; PPNAD4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125607 sequence for NM_002730 edited (data generated by NextGen Sequencing)
  ATGGGCAACGCCGCCGCCGCCAAGAAGGGCAGCGAGCAGGAGAGCGTGAAAGAATTCTTA
  GCCAAAGCCAAAGAAGATTTTCTTAAAAAATGGGAAAGTCCCGCTCAGAACACAGCCCAC
  TTGGATCAGTTTGAACGAATCAAGACCCTCGGCACGGGCTCCTTCGGGCGGGTGATGCTG
  GTGAAACACAAGGAGACCGGGAACCACTATGCCATGAAGATCCTCGACAAACAGAAGGTG
  GTGAAACTGAAACAGATCGAACACACCCTGAATGAAAAGCGCATCCTGCAAGCTGTCAAC
  TTTCCGTTCCTCGTCAAACTCGAGTTCTCCTTCAAGGACAACTCAAACTTATACATGGTC
  ATGGAGTACGTGCCCGGCGGGGAGATGTTCTCACACCTACGGCGGATCGGAAGGTTCAGT
  GAGCCCCATGCCCGTTTCTACGCGGCCCAGATCGTCCTGACCTTTGAGTATCTGCACTCG
  CTGGATCTCATCTACAGGGACCTGAAGCCGGAGAATCTGCTCATTGACCAGCAGGGCTAC
  ATTCAGGTGACAGACTTCGGTTTCGCCAAGCGCGTGAAGGGCCGCACTTGGACCTTGTGC
  GGCACCCCTGAGTACCTGGCCCCTGAGATTATCCTGAGCAAAGGCTACAACAAGGCCGTG
  GACTGGTGGGCCCTGGGGGTTCTTATCTATGAAATGGCCGCTGGCTACCCGCCCTTCTTC
  GCAGACCAGCCCATCCAGATCTATGAGAAGATCGTCTCTGGGAAGGTGCGCTTCCCTTCC
  CACTTCAGCTCTGACTTGAAGGACCTGCTGCGGAACCTCCTGCAGGTAGATCTCACCAAG
  CGCTTTGGGAACCTCAAGAATGGGGTCAACGATATCAAGAACCACAAGTGGTTTGCCACA
  ACTGACTGGATTGCCATCTACCAGAGGAAGGTGGAAGCTCCCTTCATACCAAAGTTTAAA
  GGCCCTGGGGATACGAGTAACTTTGACGACTATGAGGAAGAAGAAATCCGGGTCTCCATC
  AATGAGAAGTGTGGCAAGGAGTTTTCTGAGTTTTAG
  
  Clone variation with respect to NM_002730.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002730 unedited
  GGGATTTTGTAATACGACTCACTATAGGGCGGCCGCAATCTAGAGCGGGCGCGGAGAGAC
  GCGGGAAGCAGGGGCTGGGCGGGGGTCGCGGCGCCGCAGCTAGCGCAGCCAGCCCGAGGG
  CCGCCGCCGCCGCCGCCCAGCGCGCTCCGGGGCCGCCGGCCGCAGCCAGCACCCGCCGCG
  CCGCAGCTCCGGGACCGGCCCCGGCCGCCGCCGCCGCGATGGGCAACGCCGCCGCCGCCA
  AGAAGGGCAGCGAGCAGGAGAGCGTGAAAGAATTCTTAGCCAAAGCCAAAGAAGATTTTC
  TTAAAAAATGGGAAAGTCCCGCTCAGAACACAGCCCACTTGGATCAGTTTGAACGAATCA
  AGACCCTCGGCACGGGCTCCTTCGGGCGGGTGATGCTGGTGAAACACAAGGAGACCGGGA
  ACCACTATGCCATGAAGATCCTCGACAAACAGAAGGTGGTGAAACTGAAACAGATCGAAC
  ACACCCTGAATGAAAAGCGCATCCTGCAAGCTGTCAACTTTCCGTTCCTCGTCAAACTCG
  AGTTCTCCTTCAAGGACAACTCANACTTATACATGGTCATGGAGTACGTGCCCGGCGGGG
  AGATGTTCTCACACCTACCGCGGATCCGAAAGTTCAGTGAGCCCCATGCCCGTTTCTACC
  CGGGCCAGATCGTCCTGGCCTTTGAGTATCTGCCCTCGCTGGATCCTATCTACAGGGACC
  TGGAGCCGGAGGATCTGCTCATTGACCAGCAGGGCTACATTCAGGGACAGACTCGGGTTT
  GCCAAACGCGGGAAGGGCCCACTGGACCCTGGGCGGGACCCCGGAGACCTGGCCCCTGGA
  TTATCCGAGCAAAGGTACACAGGCCCGGGACGGGGGCCCCGGGGGCCTTATTATAGAAAG
  GCGCTGGTCACCCCCTTTTCGGAACAGCCCTCCCAATTATGAAAAACGCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002730 unedited
  CGGCCGCGAATTCGGCACGAGGTGGAACTTAAATAAGATTTTAAATTGTTGTTTTTTTAA
  AAAAATTCTAGCAAGCAACCCACTGAACATGTCACTAAAAATCTCTCCTTCCCAGGCAGG
  ATTACTCCGAAAGGAAGGTTGGCGCTTCGTTCATTTGCCCTTAGCAAGTGGGGCCTGTGG
  TTGGGTGGGATGGGGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
  
• Restriction Sites: Please inquire
• ACCN: NM_002730
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002730.3, NP_002721.1
• RefSeq Size: 2689 bp
• RefSeq ORF: 1056 bp
• Locus ID: 5566
• UniProt ID: P17612
• Cytogenetics: 19p13.12
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Hedgehog signaling pathway, Insulin signaling pathway, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Olfactory transduction, Oocyte meiosis, Prion diseases, Progesterone-mediated oocyte maturation, Taste transduction, Vascular smooth muscle contraction, Vibrio cholerae infection, Wnt signaling pathway
• Gene Summary: This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]
  Transcript Variant: This variant (1) encodes Calpha1 (PMID:21812984).