Osteopontin (SPP1) (NM_000582) Human Untagged Clone

SKU SC125352

SPP1 (untagged)-Human secreted phosphoprotein 1 (SPP1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Osteopontin
• Synonyms: BNSP; BSPI; ETA-1; OPN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125352 sequence for NM_000582 edited (data generated by NextGen Sequencing); ATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGGCATCACCTGTGCCATACCAGTTAAA; CAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGATGCTGTG; GCCACATGGCTAAACCCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGACCCTT; CCAAGTAAGTCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATGATGAC; GACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATGACACT; GATGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACT; GATTTTCCCACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGTTGTCCCCACAGTAGAC; ACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTT; CGCAGACCTGACATCCAGTACCCTGATGCTACAGACGAGGACATCACCTCACACATGGAA; AGCGAGGAGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGGACCTGAACGCGCCT; TCTGATTGGGACAGCCGTGGGAAGGACAGTTATGAAACGAGTCAGCTGGATGACCAGAGT; GCTGAAACCCACAGCCACAAGCAGTCCAGATTATATAAGCGGAAAGCCAATGATGAGAGC; AATGAGCATTCCGATGTGATTGATAGTCAGGAACTTTCCAAAGTCAGCCGTGAATTCCAC; AGCCATGAATTTCACAGCCATGAAGATATGCTGGTTGTAGACCCCAAAAGTAAGGAAGAA; GATAAACACCTGAAATTTCGTATTTCTCATGAATTAGATAGTGCATCTTCTGAGGTCAAT; TAA; ; Clone variation with respect to NM_000582.2; 240 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000582 unedited; TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACAGCATCGTCGGG; ACCAGACTCGTCTCAGGCCAGTTGCAGCCTTCTCAGCCAAACGCCGACCAAGGAAAACTC; ACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGGCATCACCTGTGCCATACCA; GTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGAT; GCTGTGGCCACATGGCTAAACCCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAG; ACCCTTCCAAGTAAGTCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAAGAT; GATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGAT; GACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTG; GTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGTTGTCCCCACA; GTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAG; AAGTTTCGCAGACCTGACATCCAGTACCCTGATGCTACAGACGANGACATCACCTCACAC; ATGGAAAAGCGAGGAGTTGAATGGTGCATACAAGGCCATCCCCGTTGCCCAGACCTNGAC; GCGCCTTCTGATTGGGACAGCCGTGGGAAGGACAGTTATGAAACGAGTCACCTGAATGAC; CAGAGTGCTGAAACCCCCAGCCCCAACCAGTCCAGAATATATAACCGGGAAGCCCATGAT; GAGAGCCATTGACCTTCCCGATGTGATTGATAGTCAGGAAACTTTCCAAAGTCGCCCGGA; ATTCCCCACCCTGAATCTCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000582 unedited; GTTGCGGCTCAGGGGATGGTCACTTCCCAGTTCCGGGAAAGCACTGAGGCAGGGGCTCAC; AGGGCATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGT; CGAGTTTTTTTTTTTTTTTTTTGCATTCAAGATAAAAGATTTATTCACACCACAAAAAGA; TAATCACAACAAAATATACACTAACTTAAAAAACAAAAGATTATAGTGTCTTAAAATGTT; ATATTCTCTTTTTAAGTGGGTAAAAGTATTTTGTTTGCTTCTACATAAATTTCTATTCGT; GAGAGAATAACAAATATTAAAATACAGTGATAGTTTGCATTTCTTCTATAGAATGAACAT; AGACATAACCCTGAAGCTTTTAGTTTACAGGGAGTTTCCATGAAGCCACAAACTAAACTA; ATTATCAAACACATTAGTTATTTCCAGACTCAAATAGATACACATTCAACCAATAAACTG; AGAAAGAAGCATTTCATGTTCTCTTTCATTTTGCTATAAAGCATTTTTTCTTTTGACTAA; ATGCAAAGAGAGAAATTGTATTTTTTCTCCTTTTAATTGACCTCAGAAGATGCACTATCT; AATTCATGAGAAATACGAAATTTCAGGTGTTTATCTTCTTCCTTACTTTTGGGGTCTACA; ACCAGCATATCTTCATGGCTGTGAAATTCATGGCTGTGGAATTCACGGCTGACTTTGGAA; AGTTCCTGACTATCAATCACATCGGAATGCTCATTGCTCTCATCATTGGCTTTCCGCTTA; TATAATCTGGACTGCTTGTGGCTGTGGGTTTCAGCACTCTGGTCATCCAGCTGACTCGTT; TCATAACTGTCCCTCCCACGGGTGGCCCAATCATAAAGCGCGTTCAGGTTCCTGAGCAAC; GGGGATGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_000582
• Insert Size: 1460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000582.2, NP_000573.1
• RefSeq Size: 1616 bp
• RefSeq ORF: 903 bp
• Locus ID: 6696
• UniProt ID: P10451
• Cytogenetics: 4q22.1
• Domains: OSTEO
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway
• Summary: The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]; Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 5. The resulting isoform (OPN-b, as described in PubMed: 7837791) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform e.