Macrophage Scavenger Receptor I (MSR1) (NM_138715) Human Untagged Clone

SKU SC125331

MSR1 (untagged)-Human macrophage scavenger receptor 1 (MSR1), transcript variant SR-AI

Product Data

• Type: Human Untagged Clone
• Target Symbol: Macrophage Scavenger Receptor I
• Synonyms: CD204; phSR1; phSR2; SCARA1; SR-A; SR-AI; SR-AII; SR-AIII; SRA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125331 sequence for NM_138715 edited (data generated by NextGen Sequencing); ATGGAGCAGTGGGATCACTTTCACAATCAACAGGAGGACACTGATAGCTGCTCCGAATCT; GTGAAATTTGATGCTCGCTCAATGACAGCTTTGCTTCCTCCGAATCCTAAAAACAGCCCT; TCCCTTCAAGAGAAACTGAAGTCCTTCAAAGCTGCACTGATTGCCCTTTACCTCCTCGTG; TTTGCAGTTCTCATCCCTCTCATTGGAATAGTGGCAGCTCAACTCCTGAAGTGGGAAACG; AAGAATTGCTCAGTTAGTTCAACTAATGCAAATGATATAACTCAAAGTCTCACGGGAAAA; GGAAATGACAGCGAAGAGGAAATGAGATTTCAAGAAGTCTTTATGGAACACATGAGCAAC; ATGGAGAAGAGAATCCAGCATATTTTAGACATGGAAGCCAACCTCATGGACACAGAGCAT; TTCCAAAATTTCAGCATGACAACTGATCAAAGATTTAATGACATTCTTCTGCAGCTAAGT; ACCTTGTTTTCCTCAGTCCAGGGACATGGGAATGCAATAGATGAAATCTCCAAGTCCTTA; ATAAGTTTGAATACCACATTGCTTGATTTGCAGCTCAACATAGAAAATCTGAATGGCAAA; ATCCAAGAGAATACCTTCAAACAACAAGAGGAAATCAGTAAATTAGAGGAGCGTGTTTAC; AATGTATCAGCAGAAATTATGGCTATGAAAGAAGAACAAGTGCATTTGGAACAGGAAATA; AAAGGAGAAGTGAAAGTACTGAATAACATCACTAATGATCTCAGACTGAAAGATTGGGAA; CATTCTCAGACCTTGAGAAATATCACTTTAATTCAAGGTCCTCCTGGACCCCCGGGTGAA; AAAGGAGATCGAGGTCCCACTGGAGAAAGTGGTCCACGAGGATTTCCAGGTCCAATAGGT; CCTCCGGGTCTTAAAGGTGATCGGGGAGCAATTGGCTTTCCTGGAAGTCGAGGACTCCCA; GGATATGCCGGAAGGCCAGGAAATTCTGGACCAAAAGGCCAGAAAGGGGAAAAGGGGAGT; GGAAACACATTAACTCCATTTACGAAAGTTCGACTGGTCGGTGGGAGCGGCCCTCACGAG; GGGAGAGTGGAGATACTCCACAGCGGCCAGTGGGGTACAATTTGTGACGATCGCTGGGAA; GTGCGCGTTGGACAGGTCGTCTGTAGGAGCTTGGGATACCCAGGTGTTCAAGCCGTGCAC; AAGGCAGCTCACTTTGGACAAGGTACTGGTCCAATATGGCTGAATGAAGTGTTTTGTTTT; GGGAGAGAATCATCTATTGAAGAATGTAAAATTCGGCAATGGGGGACAAGAGCCTGTTCA; CATTCTGAAGATGCTGGAGTCACTTGCACTTTATAA; ; Clone variation with respect to NM_138715.2; 1086 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138715 unedited; GGGGGTTCAAATTTTGTATACGACTCACTATAGGCGGCCGCGATTCGCACGAGGTCATTT; CCTTTCTTCATGTCCAGAGCTGAAATACTATGAGATAAAGATTTTAGGTTTCAATTGTAA; AGAGAGAGAAGTGGATAAATCAGTGCTGCTTTCTTTAGGACGAAAGAAGTATGGAGCAGT; GGGATCACTTTCACAATCAACAGGAGGACACTGATAGCTGCTCCGAATCTGTGAAATTTG; ATGCTCGCTCAATGACAGCTTTGCTTCCTCCGAATCCTAAAAACAGCCCTTCCCTTCAAG; AGAAACTGAAGTCCTTCAAAGCTGCACTGATTGCCCTTTACCTCCTCGTGTTTGCAGTTC; TCATCCCTCTCATTGGAATAGTGGCAGCTCAACTCCTGAAGTGGGAAACGAAGAATTGCT; CAGTTAGTTCAACTAATGCAAATGATATAACTCAAAGTCTCACGGGAAAAGGAAATGACA; GCGAAGAGGAAATGAGATTTCAAGAAGTCTTTATGGAACACATGAGCAACATGGAGAAGA; GAATCCAGCATATTTTAGACATGGAAGCCAACCTCATGGACACAGAGCATTTCCAAAATT; TCAGCATGACAACTGATCAAAGATTTAATGACATTCTTCTGCAGCTAAGTACCTTGTTTT; CCTCAGTCCAGGGACATGGGAATGCAATAGATGAAATCTCCAAGTCCTTAATAAGTTTGA; ATACCACATTGCTTGATTTGCAGCTCAACATAGAAAATCTGAATGGCAAATCCAAGAGAT; ACCTTCAAACAACAAGAGGAAATCAGTAAATTAGAGGAGCGTGTTTACCATGTATCAGCA; GAAATATGGCTATGAAAGAGGACAAGTGCATTTGGGACAGGAAATAAAGGAGAAGTGAAA; GTTCCTGATACATCACTTATGATC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_138715 unedited; GTTTTTTTTTTTAATGGGGAACCAAAGGCAATTNCAACATTNCCAAAGTAAATTAAGTCC; TCAATTTGTATTGGTGCTATTAGGTTCTTTTCTTGACTGGACTGAACTTAGTAGATTCTC; TCAAATACAAAAAGGATTTTTAGATTACTTATTAAAGATATATCTATGCATAAGTGGTAA; ATCAGCATGCATATACCATACAGCAATATAGTATTAACAACCCATAGGCACTGATCTCAG; ACATCCCAAGTGCCTATGGGTTGTCAATAGTACTATTAACAGTGTCATAGTACTAGTACT; AGTAATAGTACTAGTAATAGCAATAGTACCAGTACTAGCAATAGTACTATTATTATTTTA; AAGGTAAATTTTATTTTAAAATAAAAATAGTAATAGCAATAGTACTATTATTAGTGTTAA; TAATACTAATAGTTAATACTAACAGTTAACACTAATAGTTAATACTATTGACAACCCATA; GGCACTGATTTCAGACATCCCATGCATACCACTTACGCATAAGTAGTAAATCAGCATACA; TATACCATACAGCAATACAGTATTAACAACCCAGAGGCACTGATCTCAGACATCCCAAAC; AGGCTGACAATGCAAATGATTCTTTCAGAAACCATTGTATTTACCTTTTGAAGAAAATAC; CTAGATAGTGGAACTGAACACAAAGAATTATTAACTAGCTGAAATTACAGCCAGGGTTTG; AGACAGAAAGTGTACATTCAGCACTCATTCTCTTACCACAAAATATGCTGCCAGAGAAAA; TAGCATGTTAACAAGTACACGATGTTCATGATAAAAGCATTTCAGTAGCTGCTTACAGCA; TCCCAATTCACGAACGCTTGCCTTTTAGTTGGACGCTGGAGCCACCACTGTATATGCGGG; GAGATTAGATAGAAAAAGCTCAGCTTTGTTCATTGCCCTTTAAGAGGACGGCCCGATTCT; TTTAGGCCGTTAACATACTTCTGGGAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_138715
• Insert Size: 3780 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_138715.1, NP_619729.1
• RefSeq Size: 3682 bp
• RefSeq ORF: 1356 bp
• Locus ID: 4481
• UniProt ID: P21757
• Cytogenetics: 8p22
• Domains: Collagen, Macscav_rec, SR
• Protein Families: Druggable Genome, Transmembrane
• Summary: This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (SR-AI), also known as phSR1, encodes the longest isoform.