STAT3 (NM_139276) Human Untagged Clone

CAT#: SC124165

STAT3 (untagged)-Human signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STAT3
• Synonyms: ADMIO; ADMIO1; APRF; HIES
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_139276 edited
  GAATTCGGCACCAGCAGGCACCCCGGCTTGGCGCTGTCTCTCCCCCTCGGCTCGGAGAGG
  CCCTTCGGCCTGAGGGAGCCTCGCCGCCCGTCCCCGGCACACGCGCAGCCCCGGCCTCTC
  GGCCTCTGCCGGAGAAACAGTTGGGACCCCTGATTTTAGCAGGATGGCCCAATGGAATCA
  GCTACAGCAGCTTGACACACGGTACCTGGAGCAGCTCCATCAGCTCTACAGTGACAGCTT
  CCCAATGGAGCTGCGGCAGTTTCTGGCCCCTTGGATTGAGAGTCAAGATTGGGCATATGC
  GGCCAGCAAAGAATCACATGCCACTTTGGTGTTTCATAATCTCCTGGGAGAGATTGACCA
  GCAGTATAGCCGCTTCCTGCAAGAGTCGAATGTTCTCTATCAGCACAATCTACGAAGAAT
  CAAGCAGTTTCTTCAGAGCAGGTATCTTGAGAAGCCAATGGAGATTGCCCGGATTGTGGC
  CCGGTGCCTGTGGGAAGAATCACGCCTTCTACAGACTGCAGCCACTGCGGCCCAGCAAGG
  GGGCCAGGCCAACCACCCCACAGCAGCCGTGGTGACGGAGAAGCAGCAGATGCTGGAGCA
  GCACCTTCAGGATGTCCGGAAGAGAGTGCAGGATCTAGAACAGAAAATGAAAGTGGTAGA
  GAATCTCCAGGATGACTTTGATTTCAACTATAAAACCCTCAAGAGTCAAGGAGACATGCA
  AGATCTGAATGGAAACAACCAGTCAGTGACCAGGCAGAAGATGCAGCAGCTGGAACAGAT
  GCTCACTGCGCTGGACCAGATGCGGAGAAGCATCGTGAGTGAGCTGGCGGGGCTTTTGTC
  AGCGATGGAGTACGTGCAGAAAACTCTCACGGACGAGGAGCTGGCTGACTGGAAGAGGCG
  GCAACAGATTGCCTGCATTGGAGGCCCGCCCAACATCTGCCTAGATCGGCTAGAAAACTG
  GATAACGTCATTAGCAGAATCTCAACTTCAGACCCGTCAACAAATTAAGAAACTGGAGGA
  GTTGCAGCAAAAAGTTTCCTACAAAGGGGACCCCATTGTACAGCACCGGCCGATGCTGGA
  GGAGAGAATCGTGGAGCTGTTTAGAAACTTAATGAAAAGTGCCTTTGTGGTGGAGCGGCA
  GCCCTGCATGCCCATGCATCCTGACCGGCCCCTCGTCATCAAGACCGGCGTCCAGTTCAC
  TACTAAAGTCAGGTTGCTGGTCAAATTCCCTGAGTTGAATTATCAGCTTAAAATTAAAGT
  GTGCATTGACAAAGACTCTGGGGACGTTGCAGCTCTCAGAGGATCCCGGAAATTTAACAT
  TCTGGGCACAAACACAAAAGTGATGAACATGGAAGAATCCAACAACGGCAGCCTCTCTGC
  AGAATTCAAACACTTGACCCTGAGGGAGCAGAGATGTGGGAATGGGGGCCGAGCCAATTG
  TGATGCTTCCCTGATTGTGACTGAGGAGCTGCACCTGATCACCTTTGAGACCGAGGTGTA
  TCACCAAGGCCTCAAGATTGACCTAGAGACCCACTCCTTGCCAGTTGTGGTGATCTCCAA
  CATCTGTCAGATGCCAAATGCCTGGGCGTCCATCCTGTGGTACAACATGCTGACCAACAA
  TCCCAAGAATGTAAACTTTTTTACCAAGCCCCCAATTGGAACCTGGGATCAAGTGGCCGA
  GGTCCTGAGCTGGCAGTTCTCCTCCACCACCAAGCGAGGACTGAGCATCGAGCAGCTGAC
  TACACTGGCAGAGAAACTCTTGGGACCTGGTGTGAATTATTCAGGGTGTCAGATCACATG
  GGCTAAATTTTGCAAAGAAAACATGGCTGGCAAGGGCTTCTCCTTCTGGGTCTGGCTGGA
  CAATATCATTGACCTTGTGAAAAAGTACATCCTGGCCCTTTGGAACGAAGGGTACATCAT
  GGGCTTTATCAGTAAGGAGCGGGAGCGGGCCATCTTGAGCACTAAGCCTCCAGGCACCTT
  CCTGCTAAGATTCAGTGAAAGCAGCAAAGAAGGAGGCGTCACTTTCACTTGGGTGGAGAA
  GGACATCAGCGGTAAGACCCAGATCCAGTCCGTGGAACCATACACAAAGCAGCAGCTGAA
  CAACATGTCATTTGCTGAAATCATCATGGGCTATAAGATCATGGATGCTACCAATATCCT
  GGTGTCTCCACTGGTCTATCTCTATCCTGACATTCCCAAGGAGGAGGCATTCGGAAAGTA
  TTGTCGGCCAGAGAGCCAGGAGCATCCTGAAGCTGACCCAGGTAGCGCTGCCCCATACCT
  GAAGACCAAGTTTATCTGTGTGACACCAACGACCTGCAGCAATACCATTGACCTGCCGAT
  GTCCCCCCGCACTTTAGATTCATTGATGCAGTTTGGAAATAATGGTGAAGGTGCTGAACC
  CTCAGCAGGAGGGCAGTTTGAGTCCCTCACCTTTGACATGGAGTTGACCTCGGAGTGCGC
  TACCTCCCCCATGTGAGGAGCTGAGAACGGAAGCTGCAGAAAGATACGACTGAGGCGCCT
  ACCTGCATTCTGCCACCCCTCACACAGCCAAACCCCAGATCATCTGAAACTACTAACTTT
  GTGGTTCCAGATTTTTTTTAATCTCCTACTTCTGCTATCTTTGAGCAATCTGGGCACTTT
  TAAAAATAGAGAAATGAGTGAATGTGGGTGATCTGCTTTTATCTAAATGCAAATAAGGAT
  GTGTTCTCTGAGACCCATGATCAGGGGATGTGGCGGGGGGTGGCTAGAGGGAGAAAAAGG
  AAATGTCTTGTGTTGTTTTGTTCCCCTGCCCTCCTTTCTCAGCAGCTTTTTGTTATTGTT
  GTTGTTGTTCTTAGACAAGTGCCTCCTGGTGCCTGCGGCATCCTTCTGCCTGTTTCTGTA
  AGCAAATGCCACAGGCCACCTATAGCTACATACTCCTGGCATTGCACTTTTTAACCTTGC
  TGACATCCAAATAGAAGATAGGACTATCTAAGCCCTAGGTTTCTTTTTAAATTAAGAAAT
  AATAACAATTAAAGGGCAAAAAACACTGTATCAGCATAGCCTTTCTGTATTTAAGAAACT
  TAAGCAGCCGGGCATGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCGG
  ATCATAAGGTCAGGAGATCAAGACCATCC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_139276 unedited
  AAAAAAAAAAAAAAAAAAAAAAAAAAANNGCTGTTCACAATTGTATACGACTCCTATAGG
  CGGCCGCGNAATCGGCACCAGCAGGCACCCCGGCTTGGCGCTGTCTCTCCCCCTCGGCTC
  GGAGAGGCCCTTCGGCCTGAGGGAGCCTCGCCGCCCGTCCCCGGCACACGCGCAGCCCCG
  GCCTCTCGGCCTCTGCCGGAGAAACAGTTGGGACCCCTGATTTTAGCAGGATGGCCCAAT
  GGAATCAGCTACAGCAGCTTGACACACGGTACCTGGAGCAGCTCCATCAGCTCTACAGTG
  ACAGCTTCCCAATGGAGCTGCGGCAGTTTCTGGCCCCTTGGATTGAGAGTCAAGATTGGG
  CATATGCGGCCAGCAAAGAATCACATGCCACTTTGGTGTTTCATAATCTCCTGGGAGAGA
  TTGACCAGCAGTATAGCCGCTTCCTGCAAGAGTCGAATGTTCTCTATCAGCACAATCTAC
  GAAGAATCAAGCAGTTTCTTCAGAGCAGGTATCTTGAGAAGCCAATGGAGATTGCCCGGA
  TTGTGGCCCGGTGCCTGTGGGAAGAATCACGCCTTCTACAGACTGCAGCCACTGCGGCCC
  AGCAAGGGGGCCAGGCCAACCACCCCACAGCAGCCGTGGTGACGGAGAAGCAGCAGATGC
  TGGAGCAGCACCTTCAGGATGTCCGGAAGAGAGTGCAGGATCTAGAACAAAAATGAAAGT
  GGTAGAGAATCTCCAGGATGACTTTGATTTCAACTATAAAACCCTTAAGAGTCAAGGAGA
  CATGCCAGATCTGAATGCCAACAACCAGTCAGTGACCCCGCACCAAGATGCAGCAGCTGT
  AACAGATGCTCACTGCGCTCGACAGAATGCGAGAAACATCGTGAGTGAACTGCCGGGCCT
  TTGTCAGCGAGTGAGCCCCGCCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_139276 unedited
  CGATGATGCACCTTCCGGGCCGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGG
  GATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTGAAACAGAATCTCAAACTGTCGCCCAGGATGGAGTGCAGTGT
  GCAGTGGTGCAATTTTGGCTCACTGCAACCTCCGCCTCTCAGGTTCAAGCGATTCTCCTG
  CCTCAGCCTCCCGAGTAGCTGGGACTACAGGCCCCCACCACCCCACCCCGCTAATTTTTT
  GTACTTTTAGTAAAGACGGGGTTTCACCGTGTTAGCCCGGATGGTCTTGATCTCCTGACC
  TTATGATCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCATGCCCGG
  CTGCTTAAGTTTCTTAAATACCGAAAGGCTATGCTGATACCGTGTTTTTTGCCCTTTAAT
  TGTTATTATTTCTTAATTTAAAAAGAAACCTAGGGCTTAAATAGTCCTATCTTCTATTTT
  GATGTCAGCCAGGTTAAAAAGTGCAATGCCCGGAGTATGTAGCTATAGGTGGGCTGTGGC
  ATTTGCTTACAAAAAACAGGCAAAAGATGCCGCACGCACCAAGAAGCACTTGTCTAAAAA
  CAACAACAACAATAACAAAAAGCCTCTGAAAAAAGAGGGGCAGGGGAACAAAACACCAAC
  AAAAATTTTTCTTTTTTCTTCCTTCTTACCCCCCCCACGCCACATCCCCTGGATTTCGGG
  GTCTCAAAAGAAAACATTCCTTATTTTGCTTTTAGATAAAAGACAATCACACACATTTAC
  TCAATTCTCCATTTTAAAAATGCCTTAGTGCCTCCCGATATTCCAATTTGGATAATAAAC
  AAATTCTGCCACCCCAATTTCGTGCTCTCAAAATATCTGGGTTTCGCCCTTTTGAAGGGT
  C
  
• Restriction Sites: NotI-NotI
• ACCN: NM_139276
• Insert Size: 3384 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139276.2, NP_644805.1
• RefSeq Size: 4978 bp
• RefSeq ORF: 2313 bp
• Locus ID: 6774
• UniProt ID: P40763
• Cytogenetics: 17q21.2
• Domains: SH2, STAT
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Chemokine signaling pathway, Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer
• Gene Summary: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. This gene also plays a role in regulating host response to viral and bacterial infections. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. [provided by RefSeq, Aug 2020]
  Transcript Variant: This variant (1) represents the longest transcript, and encodes the longest isoform (1).