GPBAR1 (NM_170699) Human Untagged Clone

CAT#: SC123312

GPBAR1 (untagged)-Human G protein-coupled bile acid receptor 1 (GPBAR1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPBAR1
• Synonyms: BG37; GPCR19; GPR131; M-BAR; TGR5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001077194 edited
  GCAGAGCCTGGGTAGGAGAGCCTGGCCCCGCTGTCCCCACTGGGTGGAGACACCATGCAC
  TTGGTCCTTGTGCTCTTCAGCCAGGACACCAGACATGGTCCAAACCGCTGCAGGGCTGGC
  TGCAGCAACTCCCTGACACTCAGGAAGGCCCAGGCTGGGCAGGCAATACCTGCTCCCAAC
  AGCCATGCATGCCGGCTGCCGCTCCAGGACTCCCCTGTCCCCAGGACCAAGATGACGCCC
  AACAGCACTGGCGAGGTGCCCAGCCCCATTCCCAAGGGGGCTTTGGGGCTCTCCCTGGCC
  CTGGCAAGCCTCATCATCACCGCGAACCTGCTCCTAGCCCTGGGCATCGCCTGGGACCGC
  CGCCTGCGCAGCCCACCTGCTGGCTGCTTCTTCCTGAGCCTACTGCTGGCTGGGCTGCTC
  ACGGGTCTGGCATTGCCCACATTGCCAGGGCTGTGGAACCAGAGTCGCCGGGGTTACTGG
  TCCTGCCTCCTCGTCTACTTGGCTCCCAACTTCTCCTTCCTCTCCCTGCTTGCCAACCTC
  TTGCTGGTGCACGGGGAGCGCTACATGGCAGTCCTGAGGCCACTCCAGCCCCCTGGGAGC
  ATTCGGCTGGCCCTGCTCCTCACCTGGGCTGGTCCCCTGCTCTTTGCCAGTCTGCCCGCT
  CTGGGGTGGAACCACTGGACCCCTGGTGCCAACTGCAGCTCCCAGGCTATCTTCCCAGCC
  CCCTACCTGTACCTCGAAGTCTATGGGCTCCTGCTGCCCGCCGTGGGTGCTGCTGCCTTC
  CTCTCTGTCCGCGTGCTGGCCACTGCCCACCGCCAGCTGCAGGACATCTGCCGGCTGGAG
  CGGGCAGTGTGCCGCGATGAGCCCTCCGCCCTGGCCCGGGCCCTTACCTGGAGGCAGGCA
  AGGGCACAGGCTGGAGCCATGCTGCTCTTCGGGCTGTGCTGGGGGCCCTACGTGGCCACA
  CTGCTCCTCTCAGTCCTGGCCTATGAGCAGCGCCCGCCACTGGGGCCTGGGACACTGTTG
  TCCCTCCTCTCCCTAGGAAGTGCCAGTGCAGCGGCAGTGCCCGTAGCCATGGGGCTGGGC
  GATCAGCGCTACACAGCCCCCTGGAGGGCAGCCGCCCAAAGGTGCCTGCAGGGGCTGTGG
  GGAAGAGCCTCCCGGGACAGTCCCGGCCCCAGCATTGCCTACCACCCAAGCAGCCAAAGC
  AGTGTCGACCTGGACTTGAACTAAAGGAAGGGCCTCTGCTGACTCCTACCAGAGCATCCG
  TCCAGCTCAGCCATCCAGCCTGTCTCTACTGGGCCCCACTTCTCTGGATCAGAGACCCTG
  CCTCTGTTTGACCCCGCACTGACTGAATAAAGCTCCTCTGGCCGTTAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_170699 unedited
  TATAGGGCGGCCGAGACTTCCCGGGNTGCAGAGCCTGGTTAGGAGAGCCTGGCCCCGCTG
  TCCCCCTGGGTGGAGACACCATGCACTTGGTCCTTGTGCTCTTCAGCCAGGACACCAGAC
  ATGGTCCAAACCGCTGCAGGGCTGGCTGCAGCAACTCCCTGACACTCAGGAAGGCCCAGG
  CTGGGCTGTTTATACCTGCTCCCAACAGCCATGCATGCCGGCTGCCGCTCCAGGACTCCC
  CTGTCCCCAGGACCAAGATGACGCCCAACAGCACTGGCGAGGTGCCCAGCCCCATTCCCA
  AGGGGGCTTTGGGGCTCTCCCTGGCCCTGGCAAGCCTCATCATCACCGCGAACCTGCTCC
  TAGCCCTGGGCATCGCCTGGGACCGCCGCCTGCGCAGCCCACCTGCTGGCTGCTTCTTCC
  TGAGCCTACTGCTGGCTGGGCTGCTCACGGGTCTGGCATTGCCCACATTGCCAGGGCTGT
  GGAACCAGAGTCGCCGGGGTTACTGGTCCTGCCTCCTCGTCTACTTGGCTCCCAACTTCT
  CCTTCCTCTCCCTGCTTGCCAACCTCTTGCTGGTGCACGGNGAGCGCTACATGGCAGTCC
  TGAGGCCACTCCAGCCCCCTGGGAGCATTCGGCTGGCCCTGCTCCTCACCTGGGCTGGTC
  CCCTGCTCTTTGCCAGTCTGCCCGCTCTGGGGTGGAACCACTGGACCCCTGGTGCAACTG
  CAGCTCCCAGCTATCTTCCCAGCCCCCTACCTGTACCTCGAAGTCTATGGGCTCCTGCTG
  CCCGCCGTGGGTGCTGCTGCCTTCCTCTCTGTCCGCGTGCTGGCCACTGCCCACCGCCAG
  CTGCAGGACATCTGCCGGCTGGAGCGGGCAGTGTGCCGCGN
  
• Restriction Sites: Please inquire
• ACCN: NM_170699
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170699.1, NP_733800.1
• RefSeq Size: 1400 bp
• RefSeq ORF: 993 bp
• Locus ID: 151306
• UniProt ID: Q8TDU6
• Cytogenetics: 2q35
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 3 encode the same protein.