MED19 (NM_153450) Human Untagged Clone

SKU SC123306

MED19 (untagged)-Human mediator complex subunit 19 (MED19)

Product Data

• Type: Human Untagged Clone
• Target Symbol: MED19
• Synonyms: DT2P1G7; LCMR1; MED19AS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_153450 edited; CAGCGCCGGGGCGGAGGGTACGATGGAGAATTTCACGGCACTGTTTGGGGCTCAGGCTGA; CCCACCACCGCCCCCAACCGCACTCGGCTTCGGACCAGGAAAGCCTCCACCCCCGCCACC; GCCTCCTGCGGGCGGGGGACCCGGCACGGCCCCGCCTCCCACCGCGGCCACGGCTCCTCC; TGGCGCGGACAAGTCAGGAGCTGGCTGTGGCCCTTTTTACCTCATGAGGGAACTGCCAGG; TAGCACAGAGCTGACAGGCAGCACGAATCTGATCACACACTACAACTTGGAACAAGCCTA; TAATAAATTCTGTGGGAAGAAGGTGAAGGAGAAGCTAAGTAACTTCCTGCCTGACCTGCC; AGGGATGATTGATCTGCCTGGTTCCCATGATAACAGCAGCCTCCGCTCTCTCATTGAGAA; GCCCCCTATTCTCAGTAGCTCTTTCAATCCTATCACAGGGACCATGCTGGCCGGCTTCCG; CCTCCACACTGGCCCGTTGCCGGAGCAGTGTCGTCTGATGCATATTCAGCCTCCCAAGAA; GAAGAATAAGCACAAGCACAAACAGAGCCGTACCCAGGATCCTGTCCCCCCAGGTAAACC; CAGTTAAGTTCCGTATGGAGCTCTGGACTCCTAAATTCCCCTGAAGAAGAAACCAATGTA; CTGTGGTCTCTTGGGACTTGGGACAGGGACTCTAGCAGCACCTCTGTATCCTTTCTGGCC; ATTTGGACAAAAGCAGTTGCCCCGACTTGGGATTTTCCTGTAGTCTTCAGTGGTTCATAA; CAGTAGAGGATTTTGAGCTTTACAGGTGATAGGTACCTAATGTCTCCTACAGAAACACCA; TCTGATTCAGATCACAAGAAGAAGAAAAAGAAAAAAGAAGAGGATCCTGAACGGAAAAGG; AAGAAGAAAGAGAAGAAGAAAAAGAAGGTAGAGTAGAGGCCTATTGCCACAACCCAGTGA; GTTTCCCCAGATCTCACCCCTGTCCTAAATACCCTAGAGTTCTTTGCCTCAAGTTGGACC; CAAGATTGAAAACCCAAGGCAGGTTGCAGGTTTCTCCTGTACAGCCTGATCAAAGCCAAG; GCTAGTTCCTAGTTGTTCTTACCCTGCTTCTTTCTCCCCCAGAATCGACATAGTCCAGAC; CACCCAGGTATGGGCAGCTCCCAGGCCAGCAGCAGCAGCAGCCTACGCTAATAGGACCAC; TGGACTCTTTGCCAGGATGGCTTTTCCTGCTGTACTGAACCTGCTGATAAAAGCTGCCTT; CCAGGCTCTTGGACACTGCCTTGGGAGCATCCTGCAGCTGGGACAGAGGCCAGCTCCTGT; TGGGCTCAGTTGAGACTAAGTAAATTAGGAGAGAGAGGGACATGCTTTTGTAGGCTCATC; CCAGTTGGTTTTCTCATGGACATCTCTTCCTCTCCCAGGAAGCTTACAATTTTCTTCTCT; CTCTTTTGTGCAATTTGTCTGATTTAGGACTTGTTCTGTGTTTTCTTTAAAAAAAAAAAA; ATTACATTTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153450 unedited; NGAGTCGGATTTGTATACGACTCATATAGGGCGGCCGCGATTCCCGGGATCAGCGCCGGG; GCGGAGGGTNACGATGGAGAATTTCACGGCACTGTTTGGGGCTCAGGCTGACCCACCACC; GCCCCCAACCGCACTCGGCTTCGGACCAGGAAAGCCTCCACCCCCGCCACCGCCTCCTGC; GGGCGGGGGACCCGGCACGGCCCCGCCTCCCACCGCGGCCACGGCTCCTCCTGGCGCGGA; CAAGTCAGGAGCTGGCTGTGGCCCTTTTTACCTCATGAGGGAACTGCCAGGTAGCACAGA; GCTGACAGGCAGCACGAATCTGATCACACACTACAACTTGGAACAAGCCTATAATAAATT; CTGTGGGAAGAAGGTGAAGGAGAAGCTAAGTAACTTCCTGCCTGACCTGCCAGGGATGAT; TGATCTGCCTGGTTCCCATGATAACAGCAGCCTCCGCTCTCTCATTGAGAAGCCCCCTAT; TCTCAGTAGCTCTTTCAATCCTATCACAGGGACCATGCTGGCCGGCTTCCGCCTCCACAC; TGGCCCGTTGCCGGAGCAGTGTCGTCTGATGCATATTCAGCCTCCCAAGAAGAAGAATAA; GCACAAGCACAAACAGAGCCGTACCCAGGATCCTGTCCCCCCAGGTAAACCCAGTTAAGT; TCCGTATGGAGCTCTGGACTCCTAAATTCCNCTGAAGAAGAACCAATGTACTGTGGTCTC; TTGGGACTTGGGACAGGGACTCTAGCAGCACCTCTGTATCCTTTCTGGCCATTTGGACAA; AGCAGTTGCCCCGACTTGGATTTTCCTGTAGTCTTCAGTGGTTCATAACAGTAGAGGATT; TTGAGCTTTACAGGTGATAGGTACCCTATGTCTCCTACAGAAACACCATCTGATTCAGAT;
• Restriction Sites: Please inquire
• ACCN: NM_153450
• Insert Size: 1605 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_153450.1, NP_703151.1
• RefSeq Size: 1605 bp
• RefSeq ORF: 585 bp
• Locus ID: 219541
• UniProt ID: A0JLT2
• Cytogenetics: 11q12.1
• Protein Families: Druggable Genome
• Summary: The protein encoded by this gene is a subunit of the Mediator complex, which binds to gene-specific regulatory factors and provides support for the basal RNA polymerase II transcription machinery. This gene has been implicated in the growth of several types of cancer, and inhibition of its expression inhibits the growth and spread of these cancers. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]; Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation.